Registration Dossier
Registration Dossier
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EC number: 206-074-2 | CAS number: 299-27-4
Additional toxicological data
Administrative data
- Endpoint:
- additional toxicological information
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Study period:
- 1956
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Peer review journal
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Hog kidney gluconokinase
- Author:
- Leder IG
- Year:
- 1 957
- Bibliographic source:
- J Biol Chem 225: 125-136
Materials and methods
- Type of study / information:
- Confirmation of the formation of 6-phosphogluconate from gluconate by glucokinase extracted from hog kidney.
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Prepare enzyme extract from hog kidney. Assay the amount of 6-phosphogluconate produced by the action of the enzyme extract on gluconic acid at 34°C and pH 6.2 in the presence of ATP and Mg2+.
- GLP compliance:
- no
- Remarks:
- Work pre-dates GLP
Test material
- Reference substance name:
- gluconate
- IUPAC Name:
- gluconate
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): gluconate
Constituent 1
Results and discussion
Any other information on results incl. tables
Proportionality between enzyme concentration and activity was obtained with both assays.
The identification of the product as 6-phosphogluconate through its activity as substrate for 6-phosphogluconic dehydrogenase was confirmed by paper chromatography. The barium salt of the presumed 6-phosphogluconate was isolated by repeated alcohol precipitation and chromatographed in parallel with synthetic 6-phosphogluconate, in the methanol-formic acid and methanol-ammonia solvents described by Bandurski and Axelrod (1951). Both substances gave identical RFvalues of 0.32 and 0.57 in the respective solvents. When mixed with synthetic 6-phosphogluconate (Horecker, 1957), a single spot of the same RFwas obtained.
Reference list:
Bandurski RS, Axelrod B (1951) J Biol Chem 193:405.
Horecker BL (1957), in Colowick SP and Kaplan NO. Methods in enzymology, New York, 3, 172.
Applicant's summary and conclusion
- Executive summary:
The phosphorylation of gluconate by adenosine triphosphate (ATP) in the presence of Mg2+and an enzyme prepared from hog kidney has been studied. The enzyme, gluconokinase, has been partially purified, and the product of this reaction has been identified as 6-phosphogluconate.
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