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EC number: 262-061-1 | CAS number: 60111-54-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2012-03-22 to 2012-03-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were collected from one test chamber of each of the 37 and 73 ng/L treatment groups and the control group one day prior to the start of the test after conditioning the diluter for approximately 2 days. Samples were also collected from alternating replicate test chambers of the 37 and 73 ng/L treatment groups and the control group at the start of the test and after 48 and 96 hours to measure concentrations of the test substance.
The samples were collected, placed in glass volumetric flasks, and processed immediately for analysis. Samples of all stock solutions were also collected prior to the start of the test and at the end of the test to confirm delivery of the test substance to the test system. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Individual stock solutions (3.65, 1.85, 0.90, 0.46, 0.23 μg/mL) were prepared in DMF for each of the test concentrations by serial dilution of a master stock solution prepared at a concentration of 3.65 μg/mL. The solutions were mixed by inversion and were clear and colourless. Aliquots of each stock, sufficient for the duration of the test, were placed in the syringe pumps at the initial of the test solution flows. Any remaining stock solution was stored in amber glass bottles.
The five test substance stock solutions were injected into the diluter mixing chambers at a rate of 4.0 μL/min where they were mixed with dilution water that was being delivered at a rate of 200 mL/min, to achieve the desired test concentrations. The negative control received dilution water only. The solvent control was prepared by delivering HPLC-grade DMF to the mixing chamber at a rate sufficient to achieve a final concentration of 0.02 mL/L.
- Controls: Dilution water and Dilution water + 0.02 mL/L DMF. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: rainbow trout
- Source: Thomas Fish Company, Andersen, California 96007
- Age at study initiation: juveniles
- Length at study initiation (mean, range): 5.0 cm, 4.6-5.3 cm
- Weight at study initiation (mean and range): 1.23 g, 0.98-1.60 g
- Loading at start of test: 0.086 g fish/L/day (0.82 g fish/L instantaneous loading)
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Commercially prepared diet (Ziegler Brothers Inc., PA).
- Feeding frequency: Daily but no feed in the two days preceding the test
- Health during acclimation (any mortality observed): no adverse symptoms - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 136 mg/L as CaCO3
- Test temperature:
- 12.1 - 12.6°C
- pH:
- range 8.0 to 8.2
- Dissolved oxygen:
- ≥7.6 mg/L (≥70% ASV)
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: negative control, solvent control, 4.6 ng/l, 9.1 ng/l, 18 ng/l, 37 ng/l, 73 ng/l
Mean measured concentrations:* The analytical methodology could not measure the test substance at this concentration.
The results are expressed relative to the mean measured concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 25 L Teflon-lined stainless steel aquaria filled with 15 L of test water. Water depth was 18 cm. The aquaria were placed in a temperature-controlled water bath.
- Type: open
- Aeration: Dilution water aerated prior to use
- Type of flow-through (e.g. peristaltic or proportional diluter): Continuous flow diluter. Syringe pumps were used to deliver the test substance stock solutions or solvent to the mixing chambers. The flow of dilution water into each mixing chamber was controlled by rotameters.
- Renewal rate of test solution (frequency/flow rate): 10 volume replacements each day
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: 0.086 g fish/L/day (0.82 g fish/L instantaneous loading)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Moderately hard well water. Filtered (0.45 μm)and UV-sterilised prior to use
- Alkalinity: 174 mg/L as CaCO3
- Conductivity: 370 μS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature was monitored continuously. Dissolved oxygen and pH were measured at the start of the test, at 24-hour intervals through the test and at the end of the test. Hardness, alkalinity and conductance were measured at the start of the test.
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 16 h light, 8 h darkness
- Light intensity: 350 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : observations of mortality and other signs of toxicity were made at 2.5, 24, 48, 72 and 96 h
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 14.2 ng/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 14.2 ng/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: none reported, no overt signs of toxicity were observed
- Other biological observations: none
- Mortality of control: none
- Other adverse effects control: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: the LC50 and NOEC are around the solubility limit of the substance - Reported statistics and error estimates:
- None required - no effects were observed in any treatment
- Sublethal observations / clinical signs:
Table 1. Test results
Nominal concentration (ng/L)
Mean measured concentration (ng/L)
Percentage mortality after 96 hours
0 (Control)
Not determined*
0
0 (Solvent control)
Not determined*
0
4.6
Not determined*
0
9.1
Not determined*
0
18
Not determined*
0
37
5.1
0
73
14.2
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour LC50 value of >14.2 ng/L has been determined for the effects of the registered substance on mortality of Oncorhynchus mykiss based on mean measured concentrations of the substance. The result was obtained under flow-through test conditions.
Reference
Description of key information
LC50 (96-h): >14.2 ng/l, mortality Oncorhynchus mykiss, reliability 1, Wildlife International Ltd., 2012
Key value for chemical safety assessment
Additional information
A 96 hour LC50 value of >14.2 ng/l has been determined for the effects of the registered substance on mortality of Oncorhynchus mykiss based on mean measured concentrations of the substance in accordance with OECD TG 203 and in compliance with GLP (Wildlife International 2012). The result was obtained under flow-through test conditions.
Due to the slow hydrolysis of the test substance and the test media preparation and exposure method, it is likely that the test organisms were predominantly exposed to the parent substance.
The test substance has a water solubility of <10 ng/l and therefore, the results indicate that it is not toxic at its solubility limit.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.