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EC number: 939-071-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
One freshwater green alga study using Pseudokirchneriella subcapitata. Three studies were conducted: in two of these studies the algal media was amended with either 10 or 20 mg humic acid/L. The values from the study using 20 mg/L humic acid were more sensitive than the values from the 10 mg/L study and are the ones presented.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 4.94 mg/L
- EC10 or NOEC for freshwater algae:
- 1.3 mg/L
Additional information
The purpose of this study was to assess the effects of Paraformaldehyde, oligomeric reaction products with 4-tert-butylphenol, m-phenylenebis(methylamine), refered to as Mannich Base PTBP-MXDA, to the freshwater green alga, Pseudokirchneriella subcapitata, over a 72-hour static exposure period. These data were used to determine EL50 values for 72-hour cell density, 0-72-hour cell yield, and average specific growth rate from 0 to 72 hours. Mannich Base PTBP-MXDA has low aqueous solubility and therefore test solutions were prepared from a water accomodated fraction (WAF). Mannich Base PTBP-MXDA possesses cationic properties and was, therefore, tested in line with the OECD (2000) recommendation for testing cationic properties for the mitigating effect of 0, 10, and 20 mg/L humic acid. The results of the test are as follows:
Without Humic Acid: 72 hour cell density EL50 = 0.111 mg Mannich Base PTBP-MXDA/L; NOELR = 0.010 mg/L; 0 -72 -hour cell yield EyL50 = 0.111 mg Mannich Base PTBP-MXDA/L; NOELR = 0.010 mg/L; 0 -72 hour growth inhibition ErL50 = 0.179 mg Mannich Base PTBPMXDA/ L; NOELR = 0.010 mg/L.
With 10 mg Humic Acid/L: 72 hour cell density EL50 = 13.6 mg Mannich Base PTBP-MXDA/L; NOELR = 8.0 mg/L 0-72-hour cell yield EyL50 = 13.5 mg Mannich Base PTBP-MXDA/L; NOELR = 8.0 mg/L; 0-72 hour growth inhibition ErL50 >20 mg Mannich Base PTBP-MXDA/LNOELR = 8.0 mg/L.
With 20 mg Humic Acid/L: 72 hour cell density EL50 = 3.04 mg Mannich Base PTBP-MXDA/L; NOELR = 1.3 mg/L; 0-72-hour cell yield EyL50 = 3.09 mg Mannich Base PTBP-MXDA/L; NOELR = 1.3 mg/L; 0-72 hour growth inhibition ErL50 = 4.94 mg Mannich Base PTBP-MXDA/L; NOELR = 1.3 mg/L.
Thus, the addition of humic acid reduced the toxicity of Mannich Base PTBP-MXDA for each of the endpoints examined. The endpoints reported as the overall endpoints, and those used to derive the PNEC values will be from the study using 20 mg/L humic acid because they are the most conservative, while being the most environmentally relevant.
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