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EC number: 252-029-5 | CAS number: 34443-12-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8 August 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study was performed according to OECD Guideline 209 with GLP statement. All validity criteria were fulfilled. A significant inhibition of the respiration rate (21% relative to mean of the controls) was noted only at the highest test concentration (1000 mg/L), far greater than the limit of solubility.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None - Analytical monitoring:
- no
- Details on sampling:
- Not applicable
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Each test solution was prepared by direct addition of the test item in the test flask with an appropriate micropipette (the volume added was based on the density = 920 Kg/m3 at 20°C) and vigorous agitation of the solution for a few minutes before the beginning of the test (i.e. just before addition of the inoculum).
Groups of test and reference solutions and controls were set up as follows:
- two controls containing incoulum at 1.6 g/L;
- five test concentrations containing the test item at 10, 31.6, 100, 316 and 1000 mg/L and inoculum at 1.6 g/L;
- three reference concentrations containing the reference item 3,5-dichlorophenol at 4, 12 and 36 mg/L and inoculum at 1.6 g/L.
These solutions were prepared in test flasks and then immediately aerated for 3 hours before being transfered to an oxygen measuring apparatus. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The inoculum was left to settle and the supernatant rejected. It was then filtered (to remove the biggest particles) and washed with dechlorinated water. Four samples of 50 mL were taken to determine the content in suspended matter (dry weight). The concentratio of sludge (6.22 g/L) was then adjusted to 4 g/L by addition of the appropriate volume of dechlorinated water. The inoculum was maintained under agitation for two days before the test. For every day the sludge was not used, 50 mL of sewage feed were added, per liter. Synthetic sewage feed was prepared using dechlorinated water and analytical grade reagents following OECD recommendations.
Each concentration was prepared separately by adding 16 mL of sewage feed to each of the flasks which were made up to 300 mL with water only in the case of the controls or a nominal mixture of water and test item in the case of the test solutions. Each mixture was then made up to 500 mL by adding 200 mL of inoculum. As 200 mL of inoculum were added in a final volume of 500 mL, the organic material concentration from inoculum was 1.6 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- No
- Hardness:
- 280 +/- 20 mg/L as CaCO3
- Test temperature:
- 20°C +/- 2°C
- pH:
- The pH of the reference stock solution was 7.92 after agitation. No other informations on pH values.
- Dissolved oxygen:
- No data
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 10, 31.6, 100, 316 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: test flasks
- Type (delete if not applicable): no data
- Material, size, headspace, fill volume: no data
- Aeration: air was bubbled through each solution at the rate of 0.5 to 1 L/min until the start of oxygen concentration measurements.
- No. of organisms per vessel: inoculum at 1.6 g/L
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1 (but 2 controls. In order to verify the quality of the inoculum, the controls were prepared at the beginning (first control) and the end (second control) of the test).
- No. of vessels per vehicle control (replicates): not applicable
Solutions were prepared approximately every 15 minutes for test times of 3 hours.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
OTHER TEST CONDITIONS
- Adjustment of pH: The inoculum was buffered, if necessary, to pH 6.0 - 8.0 using sodium bicarbonate solution.
- Photoperiod: the stock vessel was kept away from light by wrapping it in opaque material.
- Light intensity: no special precautions were taken.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : After 3 hours of aeration/contact time, the content of the test flask was poured into the measuring apparatus and the oxygen concentration was determined for a period of approximately 10 minutes.Measurement of oxygen concentration of the test solutions was determined using an oxygen probe placed into an opaque BOD (Biological Oxygen Demand) flask filled with the test or reference solution which was agitated with a magnetic stirrer. The oxygen probe was connected to a meter and thence to a chart recorder.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.16
- Test concentrations: 10, 31.6, 100, 316 and 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 31.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Inhibitions of the respiration rate at 10, 31.6, 100, 316 and 1000 mg/L were 0%, 0%, 9%, 11% and 21%, respectively, relative to mean of the controls. Hence, a significant inhibition of the respiration rate (> 15% relative to mean of the controls) was noted only at 1000 mg/L.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: 3h EC50 = 11.8 mg/L (95% CL 9.73-14.2 mg/L)
- Other: none - Reported statistics and error estimates:
- No data
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under experimental conditions, the 3h-NOEC and the 3h-EC50 of the test item is 31.6 mg/L and >1000 mg/L for activated sludge, respectively.
- Executive summary:
The effect of the test item on the respiration of activated sewage sludge was evaluated using a 3h static test according to OECD guideline 209 with GLP statement.
The test solutions were prepared as follows:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/L,
- five test item solutions: activated sludge in dechlorinated water (at 1.6 g/L) and test item (to obtain final concentrations of 10, 31.6, 100, 316 and 1000 mg/L),
- three reference item (3,5 -dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/L) and reference item (to obtain final concentrations of 4, 12 and 36 mg/L).
The oxygen consumption of these solutions was measured for approximately 10 minutes, with a chart recorder, after an aeration of 3 hours. The EC50 (concentration expected to cause 50% inhibition of the respiration rate) of the test item and the reference item was determined considering the oxygen consumption of the controls as 100%.
As the validity criteria were met (the difference between the two controls was below 15% and the EC50 of the reference item was between 5 and 30 mg/L), the test was considered valid.
Inhibitions of the respiration rate at 10, 31.6, 100, 316 and 1000 mg/L were 0%, 0%, 9%, 11% and 21%, respectively, relative to mean of the controls. Hence, a significant inhibition of the respiration rate (> 15% relative to mean of the controls) was noted only at 1000 mg/L.
Under experimental conditions, the 3h-NOEC and the 3h-EC50 of the test item is 31.6 mg/L and >1000 mg/L for activated sludge, respectively.
Reference
Table 6.1.7/1: Respiration rate in mg O2/L/h
|
Concentration (mg/L) |
Respiratory rate (mg O2/L/h) |
Inhibition (%) |
Controls |
Control 1 |
42.0 |
- |
Control 2 |
44.0 |
||
Reference item |
4 |
36.0 |
16 |
12 |
19.2 |
55 |
|
36 |
8.0 |
81 |
|
Test item |
10 |
44.0 |
0 |
31.6 |
43.2 |
0 |
|
100 |
39.0 |
9 |
|
316 |
38.4 |
11 |
|
1000 |
34.0 |
21 |
Description of key information
GLP study performed according to OECD 209 guideline:
3h-NOEC = 31.6 mg/L.
3h-EC50 >1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 31.6 mg/L
Additional information
One key study is available to assess the effect of the test item on the respiration of activated sewage sludge, using a 3h static test according to OECD guideline 209 with GLP statement.
The test solutions were prepared as follows:
- two control solutions: activated sludge in dechlorinated water to obtain a final sludge concentration of 1.6 g/L,
- five test item solutions: activated sludge in dechlorinated water (at 1.6 g/L) and test item (to obtain final concentrations of 10, 31.6, 100, 316 and 1000 mg/L),
- three reference item (3,5 -dichlorophenol) solutions: activated sludge in dechlorinated water (at 1.6 g/L) and reference item (to obtain final concentrations of 4, 12 and 36 mg/L).
The oxygen consumption of these solutions was measured for approximately 10 minutes, with a chart recorder, after an aeration of 3 hours. The EC50 (concentration expected to cause 50% inhibition of the respiration rate) of the test item and the reference item was determined considering the oxygen consumption of the controls as 100%.
As the validity criteria were met (the difference between the two controls was below 15% and the EC50 of the reference item was between 5 and 30 mg/L), the test was considered valid.
Inhibitions of the respiration rate at 10, 31.6, 100, 316 and 1000 mg/L were 0%, 0%, 9%, 11% and 21%, respectively, relative to mean of the controls. Hence, a significant inhibition of the respiration rate (> 15% relative to mean of the controls) was noted only at 1000 mg/L.
Under experimental conditions, the 3h-NOEC and the 3h-EC50 of the test item is 31.6 mg/L and >1000 mg/L for activated sludge, respectively.
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