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EC number: 247-556-2 | CAS number: 26264-05-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dodecylbenzenesulphonic acid, compound with isopropylamine (1:1)
- EC Number:
- 247-556-2
- EC Name:
- Dodecylbenzenesulphonic acid, compound with isopropylamine (1:1)
- Cas Number:
- 26264-05-1
- Molecular formula:
- C18H30O3S.C3H9N
- IUPAC Name:
- sodium dodecylbenzenesulfonate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from all test concentrations and the control as follows:
Frequency: at 0 and 48 hours
Volume: 2.0 mL from the approximate centre of the test vessels
Storage: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Preparation of test solutions started with a loading rate of 100 mg/L in the combined limit/range-finding test and 46 mg/L in the final test, applying an overnight-period of magnetic stirring to ensure maximum dissolution of the test item in medium. The obtained mixture was allowed to settle for a period of three hours.
Thereafter, the aqueous Saturated Solution (SS) was collected by means of siphoning through glass wool and used as the highest test concentration. Lower test concentrations were prepared by subsequent dilutions of the SS in test medium. All test solutions were clear and colorless at the end of the preparation procedure, except for the highest test concentration of the combined limit/range-finding test which was slightly hazy and on which a layer of foam was visible. The hazy solution was checked for the presence of the Tyndall effect using a laser pen. No light scattering was observed which indicated that only the dissolved fraction of test item was present in test medium.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus, 1820
- Source: In-house laboratory culture with a known history.
- Age of parental stock (mean and range, SD): 2nd to 5th brood stock (maximum 4 weeks old)
- Feeding during test No
ACCLIMATION
- Acclimation period: Maximum 4 weeks
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: Fresh water algae
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Daphnids showing no signs of stress such as mortality >20% , presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: Daphnia, less than 24 hours old, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- Between 19 and 20°C as continuously monitored in a temperature control vessel.
- pH:
- Between 6 and 9 not varying by more than 1.5 pH units.
- Dissolved oxygen:
- >/= 3 mg/L at the end of the test.
- Salinity:
- Not specified
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Combined range-finder/limit test:
Nominal: 1, 10 and 100 mg/L
Measured, 0.53 and 7.9 mg/L (not measured at nominally 100 mg/L).
Main test:
Nominal: 1.0, 2.2, 4.6, 10.1, 22.1 and 46 mg/L.
Measured: 0.82, 1.8, 3.9, 7.7, 15 and 42 mg/L (measured concentrations at the start of the test). - Details on test conditions:
- TEST SYSTEM
- Test vessel: All glass beakers
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 60 mL beakers containing 50 mL test medium
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Standard Elendt M7 medium as per OECD guidelines fortified with salts and vitamins.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours light 8 hours darkness
- Light intensity: Not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility at 24 and 48 hours.
VEHICLE CONTROL PERFORMED: no vehicle used on study.
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: Yes; 100% immobility at nominally 10 and 100 mg/L with no immobility observed at 1 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.7 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Analytical:
Samples taken from all test concentrations and the control were analysed. The measured concentrations at the start of the test were respectively 0.82, 1.8, 3.9, 7.7, 15 and 42 mg/L in solutions containing 2.2, 4.8, 10, 22, 48 and 100% of the SS prepared at a loading rate of 46 mg/L (nominally 1.0, 2.2, 4.6, 10.1, 22.1 and 46 mg/L). During the exposure period, the concentrations remained stable, i.e. were at 87-101% of initial at the end of the test. It should be noted that measured concentrations reported were corrected for the relatively high recovery in Quality Control (QC) samples (i.e.118-131%). Additionally, a small response was measured in the control. It was assumed to result from carry-over and therefore, considered not relevant. Based on the obtained results, effect parameters were expressed in terms of initially measured concentrations.
Immobility:
No immobility was observed in the control and at the three lowest test concentrations throughout the exposure period. At the end of the test, 95% immobility was observed at the measured concentration of 7.7 mg/L, while all daphnids were immobilized at the two highest test concentrations.
The responses recorded in this test allowed for reliable determination of an EC50. - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Relevant effect levels: 48 hour EC50 0.59 mg/L (within historical laboratory range of 0.3 and 1.0 mg/L).
- Limit test: No
- Dose-response test: yes
Any other information on results incl. tables
Table 1: Incidences of mobility:
Time (h) |
Replicate |
Test substance |
||||||
Control |
0.82 |
1.8 |
3.9 |
7.7 |
15 |
42 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
D |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
|
Total introduced |
20 |
20 |
20 |
20 |
20 |
20 |
20 |
|
|
||||||||
24 |
A |
0 |
0 |
0 |
0 |
0 |
51,2 |
5 |
B |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
|
C |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
|
D |
0 |
0 |
0 |
0 |
0 |
5 |
5 |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
20 |
20 |
|
Effect % |
0 |
0 |
0 |
0 |
0 |
100 |
100 |
|
|
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
B |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
|
C |
0 |
0 |
0 |
0 |
4 |
5 |
5 |
|
D |
0 |
0 |
0 |
0 |
5 |
5 |
5 |
|
Total immobilised |
0 |
0 |
0 |
0 |
19 |
20 |
20 |
|
Effect % |
0 |
0 |
0 |
0 |
95 |
100 |
100 |
1Microscopic observation revealed no test item attached to the daphnids.
2Dissolved oxygen concentration was measured to be 8.8 mg/L directly measured after observation.
Table 2: Analytical results:
Time of sampling |
Date of sampling |
Date of |
Percentage of SS |
Analyzed concentration |
Corrected concentration |
Relative to |
0 |
09 Jul 2018 |
01 Aug 2018 |
0 |
0.0471 |
0.038 |
|
|
|
|
2.2 |
1.02 |
0.821 |
|
|
|
|
4.8 |
2.22 |
1.78 |
|
|
|
|
10 |
4.85 |
3.90 |
|
|
|
|
21.7 |
9.51 |
7.66 |
|
|
|
|
47.8 |
18.2 |
14.6 |
|
|
|
|
100 |
52.5 |
42.2 |
|
48 |
11 Jul 2018 |
01 Aug 2018 |
0 |
0.049 |
0.039 |
n.a. |
|
|
|
2.2 |
0.889 |
0.716 |
87 |
|
|
|
4.8 |
2.07 |
1.67 |
93 |
|
|
|
10 |
4.58 |
3.69 |
95 |
|
|
|
21.7 |
9.43 |
7.59 |
99 |
|
|
|
47.8 |
17.9 |
14.4 |
98 |
|
|
|
100 |
52.9 |
42.6 |
101 |
1Estimated value, calculated by extrapolation of the calibration curve. This is probably caused by carry over since a similar response was observed in the analytical blank.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 48h-EC50 for Daphnia magna exposed to the test substance was 6.7 mg/L based on initially measured concentrations (95% confidence interval between 6.1 and 7.4 mg/L).
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