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EC number: 236-921-1 | CAS number: 13548-38-4
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
Description of key information
The 14-day median lethal concentrations (LC50) of Cr(III) for earthworm range from 1656 to 1902 mg Cr/L in soil. In the organic substrate, the LC50 values of Cr(III) was 1635 mg/kg and adjusted to chromium trinitrate is 7483 mg/kg soil dw.. The NOEC values for effects of chromium on the growth and reproduction of the earthworm E. andrei in artificial soil was 32 mg Cr/kg dry soil, adjusted to 146.5 mg/kg soil dw based on chromium trinitrate.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 7 483 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil macroorganisms:
- 146.5 mg/kg soil dw
Additional information
A short-term study was conducted to evaluate the effects of Cr(III) on the survival, behavior, and morphology of the earthworm, Eisenia fetida, in water at pH 6, 7, and 8 and their toxicity in 10 different soils and an organic substrate. Six concentrations of 500, 1000, 1500, 2000, 2500, and 3000 mg Cr/kg were applied to the test animals in the definitive test. The 14-day LC50 values of Cr(III) in all soils ranged from 1656 mg/kg to 1902 mg Cr/kg at pH 6, 7 and 8. The LC50 values of Cr(III) was 1635 mg/kg in the organic substrate (LC50 of 7483 mg/kg soil dw based on chromium trinitrate). Changes were not observed in the morphology of earthworms and in their burrowing behavior in both soil and organic media at all test concentrations. The soil variables that showed significant correlations with LC50 values were considered in the development of regression equations. The equations showed that clay alone accounted for 92% of the variation in the toxicity (LC50 values) of Cr(III). The result can be read-across to chromium trinitrate as having the similar structure.
The long-term effects of the test substance on the growth and reproduction of the earthworm Eisenia andrei was determined in an artificial soil. Five concentrations of 10, 32, 100, 320 and 1000 mg Cr/kg dw and a control group were applied to the test animals. During 1 week pre-incubation period, 3 weeks period and 3 weeks recovery period, earthworm reproduction significantly reduced at soil concentrations of 100 mg Cr/kg and higher. Chromium was completely eliminated and reproduction no longer affected at the end of a 3-week recovery period. Thus, the NOEC of the test substance to test earth worms was 32 mg/kg dry soil (equivalent to 146.5 mg/kg soil dw as chromium trinitrate) and LOEC of the test substance to test earth worm was 100 mg Cr/kg dw soil.
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