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EC number: 446-620-9 | CAS number: 120983-72-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- Gas Chromatography (GC)
- Details on sampling:
- Sampling schedule of chemical analysis:
- Stock solution: at 0 hours
- Control: at 0 and 72 hours
- Test concentration/s: at 0 and 72 hours - Vehicle:
- no
- Details on test solutions:
- A stock solution was prepared to give the desired series of test concentrations. To achieve this 1.2 mg of the test substance were added to 2 litres of dilution water and treated for 60 seconds at 8000 rpm with an ultra turrax and afterwards sti1Ted for 24 h on a magnetic stirrer.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- - Source: Non-axenic strain of the test species obtained from 'The Collection of Algal Cultures' of the Institute of Plant Physiology at the University of Gottingen (Germany)
- Maintenance of stock cultures: Exponentially-growing stock cultures are maintained in the test facility under constant temperature conditions (23 +/- 2°C) at a light intensity in the range 60 - 120 μE. x m-2 x s-1 (measured in the range 400 to 700 nm using a spherical quantum flux meter). The nutrient medium (according to BRINGMANN & KUHN (1977) is renewed once a week. Cell density measurements are made using a microcell counter.
- Preparation of pre-cultures: Pre-cultures are set up three days before the start of a test. They are grown under identical exposure conditions as the stock cultures, except from the use of a different nutrient medium
- Test cultures: The algal inocula for a test are taken from an exponentially-growing pre-culture and are mixed with the nutrient medium to make up to a final
cell density of about 10E+4 cells per millilitre in the test medium. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- No data
- Test temperature:
- 21-25 °C
- pH:
- 9.4 (concerning the highest test concentration)
- Salinity:
- n.a.
- Conductivity:
- No data
- Nominal and measured concentrations:
- - Test concentration (nominally): 0.007, 0.0125, 0.025 0.05 and 0.1 mg/L
- Test concentration after 72 hours (measured): <0.0025 mg/L concerning 0.007 (n), 0.0125 (n) and 0.025 mg/L (n) and 0.0080 concerning 0.1 mg/L (n)
- Measured concentrations ranged from 64 - 94% of nominal values at O hours, and from 2.5 - 25% of nominal values at 72 hours, respectively - Details on test conditions:
- EXPOSURE CONDITIONS:
- Test vessels: 300 mL Erlenmeyer flasks with stoppers
- Culturing apparatus: Light chamber in which a temperature in the range 21 °C to 25°C can be maintained at +/- 2°C, and continuous uniform illumination is provided in the spectral range 400 to 700 nm
- Light intensity: At the average of the test solutions, a light intensity in the range 60 to 120 μE. x m-2 x s-1, or an equivalent range of 4000 to 8000 Ix, is recommended to use
- Cell density measurements: Cell densities are measured in a microcell counter or, alternatively, are determined by means of a microscopic counting chamber
- Experimental design: 5 test concentrations plus 1 control; 3 replicates per concentration, 6 replicates per control; initial cell density in the test cultures approximately 10E+4 cells per millilitre; additionally highest test concentration without algae
- Method of administration: stock solution
- Criteria of effects: substance-induced inhibition of growth and growth rate, respectively, of the algal population - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- < 0.021 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.006 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.004 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.011 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.004 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.006 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- %Concentration loss over test: 75 ... 97.5
- Validity criteria fulfilled:
- yes
- Conclusions:
- After 72 hours of exposure to the test substance an EC50 value concerning the growth rate of higher as 0.021 mg/L and lower as 0.044 mg/L was determined. The NOECr and the LOECr were 0.005 and 0.0086 mg/L, respectively. All results are expressed in terms mean measured concentrations. Measured concentrations ranged from 64 - 94% of nominal values at 0 hour, and from 2.5 - 25% of nominal values at 72 hours, respectively.
- Executive summary:
A study was performed to assess adverse effects on the growth and the growth rate of the planktonic freshwater algal species Desmodesmus subspicatus over several generations. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 3 'Algal inhibition test' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test'. Exponentially growing algal cells were exposed for a period of 72 hours to a range of concentrations, nominally 0.007, 0.0125, 0.025 0.05 and 0.1 mg/1 of test item dissolved in water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to control cultures grown under identical conditions. Growth and growth rates were used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to DUNNETT (1955, 1964). After 72 hours of exposure an EC50 value concerning the growth rate of higher as 0.021 mg/L and lower as 0.044 mg/L was determined. The NOECr and the LOECr were 0.005 and 0.0086 mg/L, respectively. All results are expressed in terms mean measured concentrations. Measured concentrations ranged from 64 - 94% of nominal values at 0 hour, and from 2.5 - 25% of nominal values at 72 hours, respectively. This toxicity study is classified as acceptable and satisfies the guideline requirements for the algae study.
Reference
Description of key information
A study was performed to assess adverse effects on the growth and the growth rate of the planktonic freshwater algal species Desmodesmus subspicatus over several generations. The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 3 'Algal inhibition test' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 201 'Alga, Growth Inhibition Test'. Exponentially growing algal cells were exposed for a period of 72 hours to a range of concentrations, nominally 0.007, 0.0125, 0.025 0.05 and 0.1 mg/1 of test item dissolved in water. Auxiliaries used to prepare the test media were an ultra turrax and a magnetic stirrer. The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to control cultures grown under identical conditions. Growth and growth rates were used to calculate a No Observed Effect Concentration and a Lowest Observed Effect Concentration according to DUNNETT (1955, 1964).After 72 hours of exposure an EC50 value concerning the growth rate of higher as 0.021 mg/L and lower as 0.044 mg/L was determined. The NOECr and the LOECr were 0.005 and 0.0086 mg/L, respectively. All results are expressed in terms mean measured concentrations. Measured concentrations ranged from 64 - 94% of nominal values at 0 hour, and from 2.5 - 25% of nominal values at 72 hours, respectively.This toxicity study is classified as acceptable and satisfies the guideline requirements for the algaestudy.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.021 mg/L
- EC10 or NOEC for freshwater algae:
- 0.006 mg/L
Additional information
"Should read: EC 50r (72 h): > 0.021 < 0.044 mg/L"
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