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EC number: 442-680-5 | CAS number: 443688-20-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July 22nd to August 22nd, 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- Version / remarks:
- 92/69/EC
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Red LF 6339
- IUPAC Name:
- Red LF 6339
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, industrial (adaptation not specified)
- Details on inoculum:
- The sludge was washed twice with tap water with centrifugation and the supernatant liquid phase was decanted. A homogenised aliquot of the final sludged suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
- Concentration of sludge: 4 g dry matter /lt; final concentration 30 mg dry material/lt
- Aeration: yes, during holding - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 94.4 mg/L
- Based on:
- test mat.
- Remarks:
- corresponds to 30.2 mg/l DOC
- Initial conc.:
- 94.3 mg/L
- Based on:
- test mat.
- Remarks:
- corresponds to 30.4 mg/l DOC
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to testing guideline
- Test temperature: 20-22 °C
- pH: measured in each test flask before the addition of the activated sludge inocolum and at the end of incubation.
- pH adjusted: yes. with a diluted sodium hydroxide solution.
- Aeration of dilution water: each flask was loosely covered with aluminium foil to allow exchange of air between the flask and the surrounding atmosphere.
- Continuous darkness: yes.
- Stirring: continuously by magnetic stirrers
TEST SYSTEM
- Culturing apparatus: 2000-ml Erlenmeyer flask, cleaned with alcoholic hydrochloric acid, rinsed with deionised water and dried. Filled with 1000 ml of test medium or test medium containing test item and/or reference item.
- Number of culture flasks/concentration: two for test item, two for procedure control, one for inoculum blank, abiotic control and toxicity control.
- Measuring equipment: DOC analysis-Shimadzu TOC-500 analyser.
SAMPLING
- Sampling frequency: day 0 (treatment day), 5, 7, 11, 14, 21 and 28 of the incubation period
- Sampling method: one sample of about 10 ml was taken from each flask per sampling date.
- Sample preparation: filtered through a 0.45 μm and analysed on the day of sampling.
CONTROL AND BLANK SYSTEM
- Inoculum blank: one replicate containing only activate sludge
- Abiotic sterile control: one replicate containing mercury dichloride. 94.0 mg/l corresponding to measured 31.7 mg/l DOC.
- Toxicity control: one replicate contating sodium benzoate (50 mg/l), test item (94.0 mg/l) and activated sludge. corresponds to 61.3 mg/l DOC.
- Procedure control: 10 ml aliquot of the stock solution and activated sludge. 50 mg/l reference item corresponding to measured 30.1 mg/l DOC and 31.2 mg/l DOC (2 replicates)
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 6
- Sampling time:
- 28 d
- Remarks on result:
- other: mean value
- Details on results:
- Points of degradation plot (test substance):
1 % degradation after 5 d
3.5 % degradation after 7 d
5.5 % degradation after 11 d
3.5 % degradation after 14 d
1 % degradation after 21 d
5.5 % degradation after 28 d
Points of degradation plot (Toxicity control)
52 % after 14 days
BOD5 / COD results
- Results with reference substance:
- Points of degradation plot (reference substance):
100 % degradation after 5 d
102 % degradation after 7 d
103 % degradation after 11 d
99 % degradation after 21 d
100 % degradation after 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- biodegradation 6 % after 28 days
- Executive summary:
The test item was investigated for its ready biodegradability in a "28-Day DOC Die-Away Test" according to EU Commission Directive 92/69 EEC, C.4-A (1992) and OECD Guideline for Testing of Chemicals, No. 301 A (1992). In the test flasks containing the test item and activated sludge (inoculum) the mean concentrations of dissolved organic the substance was found to be not biodegradable under the test conditions.
In the abiotic control, containing the test item and poisoned mineral medium, no degradation was noted after 28 days of exposure (based on DOC-measurements). The reference item sodium benzoate was completely biodegraded within 7 days of exposure, confirming the suitability of the activated sludge.In the toxicity control, containing the test item, the reference item sodium benzoate and activated sludge (inoculum), the initial DOC decreased by 52 % within 14 days of exposure. Thus, according to the test guidelines the test item can be assumed to be not inhibitory to activated sludge because degradation was >35 % within 14 days.
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