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EC number: 217-285-4 | CAS number: 1798-60-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles. The study was not conducted under GLP conditions.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The Ames II Assay is the liquid version of the classical Ames test using microwell plates. The Ames II assay is done in contrast to the classical Ames in microwell plates using a modified fluctuation test protocol. Besides the traditional tester strain TA 98 six his mutant strains TA 7001 - TA 7006 (Ames II tester strains) are used to detect base-pair substitutions.
In both tests mutations are detected by the reversion of mutations present in the amino acid requiring bacterial strains. These reversions result in the recovery of the functional capability to synthesize the essential amino acid. This gain of function leads to grow even in the absence of the amino acid required by the parent strains.
In the Ames II assay this growth leads to an accumulation of catabolites from the metabolic activity of revertants. By the catabolites the pH is dropped down which turns the reversion indicator medium from purple to yellow.
The test was carried out based on the description of Gee et al. (Mut Res 412: 115-130, 1998) and on the "Users Manual" prepared by XENOMETRIX, Inc., Colorado, USA. - GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- (R)-1-hydroxy-1-phenylacetone
- EC Number:
- 217-285-4
- EC Name:
- (R)-1-hydroxy-1-phenylacetone
- Cas Number:
- 1798-60-3
- Molecular formula:
- C9H10O2
- IUPAC Name:
- (1R)-1-hydroxy-1-phenylpropan-2-one
- Details on test material:
- - Name of test material (as cited in study report): (-)-Phenylacetylcarbinol
- Physical state: liquid
- Analytical purity: 65.47 %
- Impurities (identity and concentrations):
(according to analytical report, 2001-03-27)
- test substance: 65.47%
- Benzylalcohol: 27.953 %,
- Phenylmethylglycol 5.946 %
- Benzaldehyd, 1-Phenylpropandion-1,2 (Diketon), 1,2-Dihydroxy-2-methyl-1-phenylbutanon-3 (0.181 %)
- others: 0.453 %
- Lot/batch No.: Pa. 222/26
- Storage condition of test material: room temperature
Constituent 1
Method
- Target gene:
- his operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- other: rfa-, uvrB-, R-factor
- Species / strain / cell type:
- S. typhimurium, other: TA Mix (Mixed strains TA 7001 - TA 7006)
- Additional strain / cell type characteristics:
- other: rfa-, uvrB-, R-factor
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9-mix) prepared from the livers of male Sprague-Dawley rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- 4, 20, 100, 500, 2500, 5000 µg/mL
- Vehicle / solvent:
- DMSO was used as solvent because of the limited solubility of test substance in water.
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- metabolic activation
- Positive control substance:
- other: 2-aminoanthracene (2-AA)
- Remarks:
- for TA 98 and TA Mix; 5 µg/mL (final exposure concentration)
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- no metabolic activation
- Positive control substance:
- other: mix of 2-nitrofluorene and 4-nitroquinoline-N-oxide
- Remarks:
- for TA 98 and TA Mix; 2-nitrofluorene: 0.25 µg/mL (final exposure concentration), 4-nitroquinoline-N-oxide: 0.0625 µg/mL (final exposure concentration)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 90 min
- Exposure duration: 48 hrs
NUMBER OF REPLICATIONS: Triplicates
DETERMINATION OF CYTOTOXICITY
- Method:
1.) decreased background lawn (reduced his- background growth) leading from turbid to non-turbid purple wells
2.) decrease in number of positive wells - Evaluation criteria:
- A test is to be considered as positive when the following criteria are met:
A dose-related and reproducible increase in the number of positive wells by a factor of 2 (calculated on the basis of baseline data) in at least one tester strain either without or with S9-mix.
A test is to be considered as negative when the number of revertant wells for all tester strains were within the historical negative control range under all experimental conditions.
Each 48-well section of 384-well plates is scored for the number of revertant wells
Three scores were used:
1. An increase in the mean number of positive wells in dose groups compared to the mean values of the actual negative control. In cases of mean spontaneous mutation frequencies < 1 the mean number is corrected to a level reflecting a mean = 1 in order for further calculation of fold increase (based on mean) (1F).
2. An increase in the mean of revertant wells in dose groups calculated on the basis of the baseline data of the actual experiment. The baseline is derived from the mean spontaneous revertant number plus 1 standard deviation from the distribution of spontaneous data (baseline data/(based on mean) + standard deviation) (2F).
3. A separate baseline is derived from within each run against which data generated in that run are compared. A run consists of a number of experiments generally testing different test articles together each using a vehicle control. This leads to an accumulation of replicates for zero-dose controls which are used to calculate the mean spontaneous reversion number for each run (baseline data/ (based on mean) + standard deviation of a run) (3F).
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 98, TA Mix (Mixed strains TA 7001 - TA 7006)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- no substance precipitation was found
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Results Liquid fluctuation test
1F: mean number of positive wells in dose groups compared to the mean values of the actual negative control (description given under evaluation criteria)
2F: description given under evaluation criteria
3F: description given under evaluation criteria
TA98 strain, without microsomal activation |
|
|
||
Dose |
Mean (out of 3) |
1F |
2F |
3F |
0 |
3 ± 0 |
1 |
1 |
0.8 |
4 |
1 ± 0.0 |
0.3 |
0.3 |
0.3 |
20 |
1.3 ± 0.47 |
0.4 |
0.4 |
0.4 |
100 |
1 ± 0.0 |
0.3 |
0.3 |
0.3 |
500 |
1.3 ± 1.25 |
0.4 |
0.4 |
0.4 |
2500 |
2 ± 1.41 |
0.7 |
0.7 |
0.6 |
5000 |
2.7 ± 0.94 |
0.9 |
0.9 |
0.7 |
4-NCQ + 2-NF |
34.3 ± 2.49 |
11.4 |
11.4 |
9.6 |
|
|
|
|
|
TA98 strain, with microsomal activation |
|
|
||
Dose |
Mean (out of 3) |
1F |
2F |
3F |
0 |
2.7 ± 0.94 |
1 |
0.7 |
0.9 |
4 |
3 ± 0.82 |
1.1 |
0.8 |
1 |
20 |
0.3 ± 0.47 |
0.1 |
0.1 |
0.1 |
100 |
2.3 ± 1.7 |
0.9 |
0.6 |
0.8 |
500 |
2.0 ± 1.63 |
0.8 |
0.6 |
0.7 |
2500 |
3 ± 0.82 |
1.1 |
0.8 |
1 |
5000 |
1.0 ± 0.82 |
0.4 |
0.3 |
0.3 |
2-AA |
42.3 ± 2.05 |
15.9 |
11.7 |
14.4 |
|
|
|
|
|
|
|
|
|
|
TA Mix strain, without microsomal activation |
|
|||
Dose |
Mean (out of 3) |
1F |
2F |
3F |
0 |
1.3 ± 0.47 |
1 |
0.6 |
0.8 |
4 |
1.3 ± 1.89 |
1 |
0.6 |
0.8 |
20 |
0.7 ± 0.47 |
0.5 |
0.3 |
0.4 |
100 |
0.7 ± 0.47 |
0.5 |
0.3 |
0.4 |
500 |
0.7 ± 0.47 |
0.5 |
0.3 |
0.4 |
2500 |
0.7 ± 0.47 |
0.5 |
0.3 |
0.4 |
5000 |
0.3 ± 0.47 |
0.3 |
0.1 |
0.2 |
4-NCQ + 2-NF |
30.7 ± 0.94 |
23 |
13.5 |
19.2 |
|
|
|
|
|
TA Mix strain, with microsomal activation |
|
|
||
Dose |
Mean (out of 3) |
1F |
2F |
3F |
0 |
1.3 ± 0.47 |
1 |
0.7 |
0.7 |
4 |
1 ± 0.82 |
0.8 |
0.6 |
0.5 |
20 |
0.3 ± 0.47 |
0.3 |
0.2 |
0.2 |
100 |
0.3 ± 0.47 |
0.3 |
0.2 |
0.2 |
500 |
0.7 ± 0.47 |
0.5 |
0.4 |
0.3 |
2500 |
0.7 ± 0.47 |
0.5 |
0.4 |
0.3 |
5000 |
1.0 ± 0.82 |
0.8 |
0.6 |
0.5 |
2-AA |
30.0 ± 4.97 |
22.5 |
16.6 |
15.6 |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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