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EC number: 620-448-6 | CAS number: 566200-79-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well conducted study, performed under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- E. coli WP2 uvr A
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix (purchased from Oriental Yeast Co, Ltd.)
- Test concentrations with justification for top dose:
- Dose range-finding study: 0 (negative control). 4.88, 19.5, 78.1, 313, 1250 and 5000 micrograms/plate.
Main study: 0 (negative control), 156, 313, 625, 1250, 2500 and 5000 micrograms/plate. - Vehicle / solvent:
- - Vehicle/solvent used: DMSO
- Justification for choice of solvent/vehicle: Test substance was not soluble in distilled water (at 50 mg/l) but was soluble in DMSO (at 50mg/ml). A 50 mg/ml test substance solution with DMSO was prepared and determined to be stable (no change in colour or exothermic reaction).
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- other: DMSO (solvent) treatment assumed for negative controls
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene (2AA); 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2); Sodium azide (NaN3); 2-Methoxy-6-chloro-9-[3-(2-chloroethyl)aminopropylamino]acridine.2HCl (ICR-191)
- Details on test system and experimental conditions:
- Plates were pre-incubated at 37 +/- 0.5 degrees C for 20 minutes and then incubated at 37 +/- 0.5 degrees C for 48 hours. Colonies were counted manually and mechanically (for plates where test material precipitation was observed, colonies were counted manually, otherwise plates were counted with a colony analyzer - CA-11D, System Science Ltd.).
Results and discussion
Test resultsopen allclose all
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- See table 1 in 'Any other information on results' field
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1. Main test
With or without S9 mix | Test substance dose (ug/plate) | Number of revertant colonies per plate | |||||||||
Base-pair substitution type | Frameshift type | ||||||||||
TA100 | Mean | TA1535 | Mean | WP2uvrA | Mean | TA98 | Mean | TA1537 | Mean | ||
Without S9 | Negative control | 103 | 102 | 12 | 10 | 19 | 21 | 16 | 21 | 16 | 13 |
98 | 16 | 23 | 21 | 13 | |||||||
104 | 3 | 20 | 26 | 10 | |||||||
156 | 128 | 116 | 5 | 9 | 34 | 30 | 34 | 29 | 10 | 9 | |
104 | 12 | 25 | 23 | 7 | |||||||
313* | 78 | 99 | 11 | 11 | 18 | 19 | 20 | 17 | 9 | 8 | |
119 | 11 | 20 | 14 | 6 | |||||||
625* | 98 | 96 | 13 | 11 | 23 | 23 | 23 | 20 | 8 | 6 | |
93 | 9 | 23 | 17 | 3 | |||||||
1250* | 97 | 98 | 16 | 10 | 31 | 30 | 13 | 17 | 7 | 7 | |
99 | 4 | 28 | 20 | 7 | |||||||
2500* | 87 | 98 | 22 | 14 | 15 | 15 | 18 | 17 | 9 | 8 | |
109 | 5 | 15 | 15 | 7 | |||||||
5000* | 104 | 103 | 7 | 6 | 19 | 19 | 14 | 20 | 7 | 7 | |
101 | 5 | 18 | 26 | 6 | |||||||
With S9 | Negative control | 99 | 103 | 4 | 8 | 34 | 32 | 31 | 32 | 16 | 18 |
115 | 14 | 36 | 28 | 23 | |||||||
95 | 5 | 25 | 36 | 15 | |||||||
156 | 110 | 107 | 12 | 12 | 26 | 30 | 29 | 30 | 19 | 17 | |
103 | 12 | 33 | 30 | 15 | |||||||
313 | 117 | 119 | 14 | 13 | 38 | 31 | 30 | 34 | 19 | 23 | |
120 | 12 | 23 | 38 | 27 | |||||||
625 | 121 | 116 | 10 | 8 | 42 | 38 | 26 | 30 | 27 | 27 | |
110 | 5 | 33 | 34 | 27 | |||||||
1250* | 117 | 127 | 8 | 9 | 30 | 30 | 20 | 22 | 27 | 25 | |
137 | 9 | 30 | 24 | 22 | |||||||
2500* | 126 | 113 | 6 | 8 | 21 | 27 | 27 | 24 | 28 | 24 | |
100 | 10 | 32 | 20 | 19 | |||||||
5000* | 102 | 104 | 5 | 7 | 24 | 27 | 23 | 27 | 15 | 16 | |
106 | 9 | 29 | 30 | 16 | |||||||
Positive control without S9 | Chemical | AF-2 | NaN3 | AF-2 | AF-2 | ICR-191 | |||||
Dose (ug/plate) | 0.01 | 0.5 | 0.01 | 0.1 | 0.5 | ||||||
No. of revertants colonies/plate | 629 | 625 | 350 | 330 | 280 | 259 | 485 | 482 | 1284 | 1350 | |
621 | 310 | 237 | 478 | 1416 | |||||||
Positive control with S9 | Chemical | 2AA | 2AA | 2AA | 2AA | 2AA | |||||
Dose (ug/plate) | 1 | 2 | 10 | 0.5 | 2 | ||||||
No. of revertants colonies/plate | 1063 | 1035 | 249 | 248 | 657 | 691 | 265 | 253 | 226 | 209 | |
1007 | 247 | 724 | 240 | 191 |
* Precipitate of test substance was observed in all plates for each test strain
2-Aminoanthracene (2AA); 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide (AF-2); Sodium azide (NaN3); 2-Methoxy-6-chloro-9-[3-(2-chloroethyl)aminopropylamino]acridine.2HCl (ICR-191)
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Under the conditions of this study, S-DHMN gave a negative response in S.typhimurium strains TA1535, TA1537, TA98 and TA100 and E.coli strain WP2uvrA in both the presence and absence of S9-mix.
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