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EC number: 232-019-7 | CAS number: 7783-66-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data published in a peer-reviewed journal, adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Lack of genotoxicity of potassium iodate in the alkaline comet assay and in the cytokinesis-block micronucleus test. Comparison to potassium bromate
- Author:
- Poul, J.M. et al.
- Year:
- 2 004
- Bibliographic source:
- Food and Chemical Toxicology 42 (2004) 203–209
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Comet assay (single cell gel electrophoresis assay)
- GLP compliance:
- no
- Type of assay:
- other: Comet assay
Test material
- Reference substance name:
- Potassium iodate
- EC Number:
- 231-831-9
- EC Name:
- Potassium iodate
- Cas Number:
- 7758-05-6
- IUPAC Name:
- potassium iodate
- Details on test material:
- - Supplier: Acros organics
- Purity: >99%
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Chinese hamster Ovary (CHO) K1
- Details on mammalian cell type (if applicable):
- Media: HAM’S F12 medium with L-glutamine supplemented with 10% fetal calf serum, penicillin (50 UI/ml) and streptomycine (50 mg/mL).
- Test concentrations with justification for top dose:
- Preliminary cytotoxicity assays: Concentrations ranging from 0.001 to 5 mg/mL for 1 hour.
Comet assay: Cells were exposed for 3 hours to 0.625, 1.25, 2.5, 5 and 10 mM.
Stability of iodate under exposure conditions was confirmed by analysis (titrimetric method after reduction of iodate to elemental iodine). - Vehicle / solvent:
- Culture medium
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: etoposide
- Details on test system and experimental conditions:
- Cells were collected by trypsination, suspended in prewarmed low melting point (LMP) agarose (0.5% in PBS) and deposited on a conventional microscope slide (initially dipped in 1% agarose and dried) precoated with normal agarose (0.8% in PBS). Slides were put in a lysis solution (2.5 M NaCl, 0.1 M EDTA, 10 mM Tris pH10, 10% DMSO and 1% Triton 100) for 1 hour at about 5°C. DNA was allowed to unwind in electrophoresis buffer (0.3 M NaOH, 1 mM EDTA, pH13.6) for 40 min at room temperature. Slides were then placed into a horizontal electrophoresis tank (Hoeffer HE99) and exposed to 0.7 V/cm (300 mA) for 24 minutes. After electrophoresis, slides were washed twice in neutralization buffer (0.4 M Tris, pH7.5) and dehydrated in ethanol for 5 minutes. After staining with ethidium bromide, 50 randomly selected cells per slide were submitted to image analysis (Module CometPro4 of Aphelion software, ADCIS society, Caen, France). Each dose was tested in duplicate and at least two independent assays were performed. Etoposide (0.5 mg/mL), a well known inhibitor of topoisomerase II inducing DNA double strand breaks was used as positive control. In parallel to the assessment of DNA damage, cell viability was measured using the Trypan blue exclusion method. Cell viability was expressed as proportion of total cells.
- Evaluation criteria:
- Olive tail moment (OTM), defined as the product of the distance between the barycentres of the head and tail by the proportion of DNA in the tail, was used to evaluate the extent of DNA damage in individual cells. Highly damaged cells (HDC) were characterized by an extensive DNA fragmentation which allowed 90% of the DNA to migrate during electrophoresis, forming the comet tail. Median values of OTM were calculated without taking HDC into account.
- Statistics:
- Data were expressed as the median values of OTM (±S.D.) for each slide.
Comparisons between control and treated cell cultures were made using ANOVA and Dunnett’s one sided test.
Results and discussion
Test results
- Species / strain:
- not specified
- Metabolic activation:
- not applicable
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- CYTOTOXICITY
- Preliminary experiment: Cell mortality was found <5% with the exception of cell cultures exposed to the highest concentration (5 mg/mL) of potassium iodate (13.0%).
- None of the concentrations used in the comet assay increased the mortality over 6%.
GENOTOXICITY
- No primary DNA damage was observed after cell exposure to potassium iodate. There was a slight increase in tail moment observed for the 10 mM potassium iodate concentration, however, this was not statistically significant from control. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
Potassium iodate was not genotoxic in an alkaline comet assay with CHO cells. - Executive summary:
The genotoxic potential of potassium iodate was investigated in vitro, using the alkaline comet assay with CHO cells. No DNA damage was detected in cells exposed to potassium iodate for 3 hours to concentrations up to 10 mM.
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