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EC number: 620-056-5 | CAS number: 874195-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 Jun - 13 Jul 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Gesundheit und Soziales des Landes Nordrhein-Westfalen
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- To obtain the nominal test concentrations of 1000, 562, 316, 178 and 100 mg a.s./kg dry weight soil, nominal amounts of 638, 359, 202, 114 and 64 mg test item were weighed into a petri dish respectively and quartz sand (washed and calcined) was added up to a final weight of 5 grams for each of the four replicates. Test item and quartz sand were transferred to a mortar and thoroughly mixed with a pestle. Each application mixture (5 g) was transferred separately to artificial soil (595 g dry weight) and mixed thoroughly using a laboratory mixer. To the control replicates 5 g of quartz sand (without test item) was mixed into the soil. Finally 59.5 mL of deionised water were added and mixed into each replicate to achieve between 40 % and 60 % of the maximum water holding capacity (WHCmax).
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Strain: Eisenia fetida andrei
- Common name: Earth worm
- Source: Laboratory culture, original source Prof. Graff, Forschungsanstalt für Landwirtschaft, 38104 Braunschweig, Germany
- Age at test initiation: Adult worms (more than two months old)
- Weight at test initiation: 0.34g (average)
BREEDING CONDITIONS
- According to BBA Proposal for OECD (ECO 85, = UPEC 15, February 1981)
- Temperature: 22 ±2 °C
- Light cycle: 12:12 hours light-dark
- Substrate: 70 % natural soil, 25 % peat and 5 % straw (dry weight in each case), animals transferred into fresh substrate at half yearly intervals
- Feeding: Dried cattle manure at 14 day intervals
ACCLIMATION
- Acclimation period: 24 hours
- Acclimation conditions: yes - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.0 - 6.1
- Moisture:
- water content in the artificial soil: 25.7 - 26.2 %
water content as % of WHCmax: 56.7 - 58.2 % - Details on test conditions:
- TEST SYSTEM
- Test container: 1.5 liter preserving jars, covered with glass lids
- Amount of soil or substrate: 595 g dry weight test soil, resulting in approximately 803 g wet artificial soil
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
PROPERTIES OF SUBSTRATE
- Composition: 10% Sphagnum peat, 20% Kaolinite clay, 69.6% industrial quartz sand (Sort: F 36), 0.4% Calcium carbonate
- Stability and homogeneity of test material in the medium: The dry components intensely mxed in a cement mixer
- Maximum water holding capacity: wetted with deionised water to reach to a content of 40 % - 60 % of the maximum water holding capacity
OTHER TEST CONDITIONS
- Photoperiod: constant light
- Light intensity: 400 - 800 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Seven days after the start of the study, the number of surviving earthworms was determined by spreading the soil onto an inert surface and removing the earthworms by hand. The surviving animals were then returned to the test container with the test soil. After 14 days, the weight, abnormal behaviour, observed symptoms as well as the number of surviving earthworms were determined. Earthworms which showed no reaction when prodded with a blunt probe were considered dead. - Nominal and measured concentrations:
- Nominal test concentrations: 1000, 562, 316, 178 and 100 mg a.s./kg dry weight soil
- Reference substance (positive control):
- yes
- Remarks:
- Chloroacetamide
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- No morphological and behavioural effects were observed.
Based on weight the Lowest Observed Effect Concentration (LOEC) was determined as 100 mg/kg soil dw. However, to a test concentration of 100 mg a.s./kg weight soil, the weight difference between control and treatment group were within 5 %. These weight alterations are considered to be well in the range of biological variation. - Results with reference substance (positive control):
- The LC50 (14 days) was calculated to be 26.1 mg Chloroacetamide A.R. /kg dry weight soil (95 % confidence limits: 18.9 – 43.8 mg Chloroacetamide A.R. /kg dry weight soil). The LC50 was within the concentration range determined in international ring studies.
Reference
Table 1: Effects on the test organism
|
Mortality (%) |
Weight |
||
7 days |
14 days |
% mean |
significance according to Williams-Test |
|
control |
0 |
0 |
+4 |
|
100 mg/kg |
0 |
0 |
+1 |
+ |
178 mg/kg |
0 |
0 |
-3 |
+ |
316 mg/kg |
0 |
0 |
-5 |
+ |
562 mg/kg |
0 |
0 |
-5 |
+ |
1000 mg/kg |
0 |
0 |
-9 |
+ |
Description of key information
Eisenia fetida LC50 (14d) > 1000mg a.s./kg soil dw. (nominal)
Key value for chemical safety assessment
Additional information
One study is available on the short-term toxicity of triafamone (CAS No. 874195-61-6) to the earth worm Eisenia fetida (Leicher, 2010). The study was performed according to OECD guideline 207 and GLP. Based on weight the Lowest Observed Effect Concentration (LOEC) was determined as 100 mg a.s./kg soil dw. However, the weight difference between control and treatment group were within 5 %. These weight alterations are considered to be well in the range of biological variation. No mortality and no morphological and behavioural effects were observed during the 14 days test duration. Therefore the LC50 (14d) is above the highest test concentration of 1000 mg/kg soil dw.
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