Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-155-7 | CAS number: 116-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: well performed study according to OECD guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
Test material
- Reference substance name:
- 1,4-bis(mesitylamino)anthraquinone
- EC Number:
- 204-155-7
- EC Name:
- 1,4-bis(mesitylamino)anthraquinone
- Cas Number:
- 116-75-6
- Molecular formula:
- C32H30N2O2
- IUPAC Name:
- 1,4-bis(mesitylamino)anthraquinone
Constituent 1
- Radiolabelling:
- no
Study design
HPLC method
- Details on study design: HPLC method:
- Procedure:
The distribution coefficient was estimated by the HPLC method using isocratic elution. The HPLC system was calibrated for log k' versus log Koc using reference substances of known log Koc.
Test substance solution: 14,8 mg in 10 ml methanol, filtered through 0,45 μm PTFE membrane filter
Calibration solutions: approx. 5-10 μg/ml in acetonitrile (see 4.3)
Dead time marker: sodium nitrate.
Instrumental conditions:
HPLC instrument: Agilent 1100
Injection volume: 2 μl
Detector: UV, 254 nm (test substance) and 220 nm (calibration)
Column: μBondapak CN, 150 mm x 3,1 mm, 10 μm
Mobile phase: water/methanol = 45/55 (v/v)
Flow rate: 1 ml/min
Column temperature: 40 °C
Results and discussion
Adsorption coefficient
- Type:
- log Koc
- Value:
- 7.7
- Temp.:
- 40 °C
Results: HPLC method
- Details on results (HPLC method):
- Reference substances for calibration
Compound Calibr. Purity [%] log Koc Conc.
solution No. value [μg/ml]
Sodium nitrate 1 > 99 % --- 10
Acetanilide 2 99,9 1,25 11
Phenol 1 99,8 1,32 10
Methylbenzoate 2 98,7 1,80 9
Naphthalene 2 99,4 2,75 2
1,2,3-Trichlorobenzene 1 99,8 3,16 10
Phenanthrene 2 >97 4,09 5
4,4'-DDT solution 1 100 5,63 5
(100 ng/μl)
Analytical data
Substance log Koc Inj. 1 Inj. 2 k' log k' mass on
value tR [min] tR [min] column [ng]
Sodium nitrate --- 0,770 0,771 --- --- 20
Acetanilide 1,25 1,734 1,733 1,25 0,10 22
Phenol 1,32 1,742 1,742 1,26 0,10 21
Methylbenzoate 1,80 1,895 1,895 1,46 0,16 17
Naphthalene 2,75 2,402 2,401 2,12 0,33 4
1,2,3-Trichlorobenzene 3,16 2,479 2,480 2,22 0,35 19
Phenanthrene 4,09 3,237 3,236 3,20 0,51 11
4,4'-DDT 5,63 4,952 4,954 5,43 0,73 10
Test substance --- 8,996 8,886 10,61 1,03 *
Estimated log Koc of test substance:
log Koc = (log k'-b)/a = 1.03 -(-0.0926)/0.1461 = 7.7
a and b were taken from the calibration curve of the substances with known Log Koc
The calculated log Koc value of the test substance is outside the calibrated range. 4,4'-DDT is
the reference substance with highest log Koc value available. Therefore only an extrapolated
value for the test substance can be obtained.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The calculated extrapolated value for the Log Koc of the test item is 7.7
- Executive summary:
The estimation of the adsorption coefficient (Koc) on soil and sewage sludge was performed using high performance liquid chromatography (HPLC) according to OECD guideline 121. Sodium nitrate, Acetanilide, Phenol, Methylbenzoate, Naphthalene, 1,2,3-Trichlorobenzene, Phenanthrene, 4,4'-DDT with known LogKoc were used as standard to establish a calibration curve with the retentions times. The retention time was compared with the standards. The calculated log Koc value of the test substance is outside the calibrated range. 4,4'-DDT is the reference substance with highest log Koc value available. Therefore only an extrapolated value for the test substance can be obtained.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.