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EC number: 263-064-0 | CAS number: 61789-51-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Reasonably well conducted study. Positive controls are not given. Test procedure with modification of the standard LLNA, but according to the author, the used method have comparable sensitivity to the radioactive original method.
Data source
Reference
- Reference Type:
- publication
- Title:
- Allerginicity and cross-reactivity of naphthenic acid and ist metallic salts in experimental animals.
- Author:
- Yamano, T.; et al.
- Year:
- 2 006
- Bibliographic source:
- Contact Derm. 54, 25-28
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The method to test the reactivity of CoCl2 was performed by the modified method of the standard LLNA with some modification.
- GLP compliance:
- no
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- Naphthenic acids, cobalt salts
- EC Number:
- 263-064-0
- EC Name:
- Naphthenic acids, cobalt salts
- Cas Number:
- 61789-51-3
- IUPAC Name:
- λ²-cobalt(2+) bis(3-(3-ethylcyclopentyl)propanoate)
- Details on test material:
- - Name of test material (as cited in study report): cobalt naphthenate
- Molecular weight (if other than submission substance): 397.42 g/mol
- Physical state: solid
No further details are given.
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: CLEA Japan (Tokyo, Japan)
No details are given.
Study design: in vivo (LLNA)
- Vehicle:
- other: petrolatum
- Concentration:
- 25 µL of 0.3%, 1%, 3% or 10%
- No. of animals per dose:
- 4 mice
- Details on study design:
- RANGE FINDING TESTS:
No data available, if a range finding test was performed.
MAIN STUDY
TREATMENT PREPARATION AND ADMINISTRATION: Mice were exposed to 25 µL of various concentrations through application to the dorsum of both ears for 3 consecutive days (days 0-2). On day 4, 5-bromo-2'-deoxyuridine (BrdU) was administered i.p. to each mouse. The next day, a pair of auricular lymph nodes from each mouse was excised. After counting the total cell numbers from each mouse, BrdU concentrations were measured by enzyme-linked immunosorbent assay.
EVALUATION
Total lymph node cell count in a dosed animal divided by mean lymph node cell count in a control group was designated as a cellularity index, whereas BrdU incorporation per unit number of cells from a dosed animal divided by mean BrdU incorporation from a control group was used as a BrdU.incorporation index. A LLNA-stimulation index was calculated by multiplying the cellularity indey by the BrdU-incorporation index. At the time of lymph node excision, the thickness of the ears was measured with a digital micrometer. Ear thickness of a dosed animal divided by mean ear thickness of a control group was designated as ear-thickness index. - Positive control substance(s):
- not specified
- Statistics:
- Index values for each dosed group and vehicle-control group were compared via Dunnett's or Steel's multiple comparison method using a Statlight software package.
Results and discussion
- Positive control results:
- no data
In vivo (LLNA)
Resultsopen allclose all
- Key result
- Parameter:
- SI
- Value:
- ca. 1.75
- Test group / Remarks:
- 1 % concentration
- Remarks on result:
- other: Test substance exhibited significant increase in the LLNA-stimulation index values at the respective highest doses tested. Minimum induction dose for Cobalt naphthenate was 1% (see attached figure).
- Key result
- Parameter:
- SI
- Value:
- ca. 12
- Test group / Remarks:
- 3 % concentration
- Key result
- Parameter:
- SI
- Value:
- ca. 12.5
- Test group / Remarks:
- 10 % concentration
Applicant's summary and conclusion
- Interpretation of results:
- other: potential skin sensitiser
- Conclusions:
- Cobalt naphthenate applied in concentration >1% can be evaluated as a potential skin sensitiser.
- Executive summary:
Female BALB/c mice (4 per group) were treated with 25 µl of various concentrations (0.3, 1, 3 and 10 %) of Cobalt naphthenate in pet. or with petrolatum alone (vehicle control). The test substance was applied to the dorsum of both ears for 3 days. On day 4, 5-bromo-2'-deoxyuridine (BrdU) was administered i.p. The next day, a pair of auricular lymph nodes were excised. Total cell numer was counted and total BrdU concentrations were measured by enzyme-linked immunosorbent assay.A stimulation index was determined: BrdU incorporation per unit number of cells from treated animals compared to controls.
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