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EC number: 294-785-9 | CAS number: 91770-03-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 1993-11-15 to 1994-07-08
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- acceptable restriction was that analytical measurements on the test material were not conducted.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Principles of method if other than guideline:
- not applicable.
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 1, 10, 100, 1000, and 10000 ppm
- Sampling method: The sludge/microbial inoculum was exposed to the test material. To measure the rate of oxygen consumption for the activated sludge after exposure to the test materials a Model 51 Yellow Springs Instruments dissolved oxygen meter was used. The oxygen meter was fitted with a BOD bottle probe, with a standard sensitivity membrane and an external output to a Fisher Recordal strip chart recorder. To measure the consumption of oxygen, a sub-sample of the exposed sludge was placed in a clean BOD bottle, the change in oxygen concentration over time was measured with the YSI meter and probe, and the results were recorded on the strip chart recorder.
- Sample storage conditions before analysis: none - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: As the test material was considered insoluble, no stock solution was prepared and the material concentrations were 1, 10, 100, 1000, 10000. Thus for 1 mg/L, 0.0005 g was added to the test vessel; for 10 mg/L, 0.0050 g; for 100 mg/L 0.05 g; for 1000 mg/L 0.5 g; and for 10000 mg/L 5.0 g. Each vessel was set up at 15 min intervals.
- Eluate: The dilution water for this test was from the City of Franklin, Tennessee, water supply. The water was softened and dechlorinated prior to use.
- Differential loading: see above.
- Controls: After the last test material vessel was prepared, a vessel with no test material was prepared as a test control replicate.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Method of cultivation: Immediately upon arrival, the sludge was aerated with low-pressure, oil-free air. The activated sludge organisms were fed a synthtic sewage feed at a rate of 50 mL per liter.
- Preparation of inoculum for exposure: Triplicate 4-mL samples of the mixed sludge were dried at 100°C in a Fisherbrand drying oven, until a constant weight was achieved. Based on these results the sludge was diluted to produce a dry weight per unit volume concentration of 4 g/L. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- none
- Hardness:
- no data
- Test temperature:
- 20 +/- 1°C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- not applicable
- Nominal and measured concentrations:
- nominal concentrations: 1, 10, 100, 1000, and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: flint glass bottles
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 1 L
- Aeration: yes
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: in the dark
- Light intensity: not applicable
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol (97% pure)
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- none
- Results with reference substance (positive control):
- - Results with reference substance valid: yes
- Relevant effect levels: EC50(3h) = 16 mg/L, i.e. in the acceptable range of 5-30 mg/L.
- Other: none - Reported statistics and error estimates:
- none
- Validity criteria fulfilled:
- yes
- Conclusions:
- The respiration rates of the sludge-associated microbes exposed to the 5 nominal concentrations of test item were 36.9, 31.7, 31.8, 29.5, and 20.2 mg O2/L*hr respectively. The EC50 was calculated to be greater than 10,000 mg/L.
- Executive summary:
Guideline: The potential impact of the substance on microbial metabolism, as represented by the consumption of oxygen, was investigated using the "Activated Sludge, Respiration Inhibition Test" as prescribed by OECD (1984) and detailed in WCC Protocol OECD 209 (Expanded Range Procedures).
Methods: The test was performed on February 15, 1994. The test duration was a 3-h exposure period to the test material followed by up to 10 minutes for the measurement of oxygen consumption. The study design was comprised of 5 nominal exposure concentrations: 1, 10, 100, 1000, and 10000 ppm; a duplicated control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5-dichlorophenol).
Results: The activated sludge respiration test with test item passed the quality control criteria for an acceptable test. The EC50 calculated for the reference toxicant was 16.2 mg/L, within the acceptable range of 5 to 30 mg/L. The 2 control replicates produced oxygen consumption rates within the required 15% of each other, 33.4 and 33.0 mg O2/L x hour. The respiration rates of the sludge-associated microbes exposed to the 5 nominal concentrations of the substance were 36.9, 31.7, 31.8, 29.5, and 20.2 mg O2/L*hr respectively. The EC50 was calculated to be greater than 10,000 mg/L.
Reference
No other information.
Description of key information
The respiration rates of the sludge-associated microbes exposed to the 5 nominal concentrations of the substance were 36.9, 31.7, 31.8, 29.5, and 20.2 mg O2/L*hr respectively. The EC50 was calculated to be greater than 10,000 mg/L (OECD 209).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
Additional information
Guideline: The potential impact of the substance on microbial metabolism, as represented by the consumption of oxygen, was investigated using the "Activated Sludge, Respiration Inhibition Test" as prescribed by OECD (1984) and detailed in WCC Protocol OECD 209 (Expanded Range Procedures).
Methods: The test was performed on February 15, 1994. The test duration was a 3-h exposure period to the test material followed by up to 10 minutes for the measurement of oxygen consumption. The study design was comprised of 5 nominal exposure concentrations: 1, 10, 100, 1000, and 10000 ppm; a duplicated control group; and an assessment of the sensitivity of the inoculum used in the test to a reference toxicant (3,5-dichlorophenol).
Results: The activated sludge respiration test with test item passed the quality control criteria for an acceptable test. The EC50 calculated for the reference toxicant was 16.2 mg/L, within the acceptable range of 5 to 30 mg/L. The 2 control replicates produced oxygen consumption rates within the required 15% of each other, 33.4 and 33.0 mg O2/L x hour. The respiration rates of the sludge-associated microbes exposed to the 5 nominal concentrations of the substance were 36.9, 31.7, 31.8, 29.5, and 20.2 mg O2/L*hr respectively. The EC50 was calculated to be greater than 10,000 mg/L.
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