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EC number: 269-915-2 | CAS number: 68390-97-6 This substance is identified by SDA Substance Name: C16-C18 alkyl dimethyl amine and SDA Reporting Number: 19-040-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details on endpoint-specific justification, please see read-across justification document (analogue approach) in section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- assessment report
- Analytical monitoring:
- no
- Details on sampling:
- No analytical monitoring
- Vehicle:
- no
- Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 19 - 20 °C
- Nominal and measured concentrations:
- 1000 - 400 - 160 - 64 - 25.6 mg/kg soil dry weight
- Reference substance (positive control):
- yes
- Remarks:
- Cyanoguanidin
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 941 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: RA from HYEQS; value converted based on MW
- Basis for effect:
- nitrate formation rate
- Remarks on result:
- other: Orig. value source: 1000 mg/kg dw
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: No
- Results with reference substance (positive control):
- Mean Nitrate-N Content of the Reference Item [mg N/kg Soil Dry Weight]
Test concentrations
[mg/kg soil dry weight] Mean Nitrate-N Content [mg N/kg soil dry weight]
0 d 7d 14 d 28 d
Control 8.70 14.7 17.7 27.6
50 8.21 10.6 10.9 13.7
100 8.00 9.26 10.2 10.1
Changes of the Nitrate-N Formation Rates of Reference Item Soils Compared to the
Control Soils [%]
Formation Rates
Day 7 14 28
Cyanoguanidine 50 mg/kg soil dry weight 60 70 72
Cyanoguanidine 100 mg/kg soil dry weight 79 75 90 - Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA) with following DUNNETT’s test were carried out for the determination of statistically significant differences compared to control replicates. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value for ANOVA and DUNNETT’s test (acceptable probability of incorrectly concluding that there is a difference) is a = 0.05.
EC25 and EC50 values were calculated by sigmoidal dose-response regression. Calculations of the confidence intervals for EC25 and EC50 were carried out using standard procedures. - Validity criteria fulfilled:
- yes
- Executive summary:
The study used as source investigated the toxicity of HYEQS in a Soil Microorganisms: Nitrogen Transformation Test (OECD 216). The study results of the source substance were considered applicable to the target substance. Justification and applicability of the read-across approach (structural analogue) is outlined in the read-across report in section 13.
Reference
Nitrate-N Contents in Comparison to the Untreated Soil [%]
Test concentration |
Nitrate-N Contents [%] |
|||
[mg/kg soil dry weight] |
0 d |
7 d |
14 d |
28 d |
25.6 |
-2 |
-2 |
-5 |
4 |
64 |
6 |
4 |
-2 |
2 |
160 |
1 |
6 |
-5 |
2 |
400 |
4 |
1 |
10 |
6 |
1000 |
5 |
16 |
24 |
8 |
Nitrate-N Formation Rates of Test Item Soils Compared to the Untreated Soil [%]
Test concentration [mg/kg soil dry weight] |
Nitrate-N Formation Rates [%] |
||
7 d |
14 d |
28 d |
|
25.6 |
-4 |
-10 |
9 |
64 |
2 |
-15 |
-2 |
160 |
16 |
-14 |
5 |
400 |
-5 |
-18 |
9 |
1000 |
36* |
51* |
11 |
Nitrate-N Formation Rate: NOEC, LOEC and EC- Values with Confidence Intervals
Day 7 |
Day 14 |
Day 28 |
|
[mg/kg soil dry weight] |
|||
EC50 |
> 1000 |
908 |
> 1000 |
95 % confidence interval |
– |
738 - > 1000 |
– |
EC25 |
782 |
562 |
> 1000 |
95 % confidence interval |
657 - 955 |
465 - 688 |
– |
LOEC |
1000 |
1000 |
> 1000 |
NOEC |
400 |
400 |
1000 |
Nitrate-N Contents - Day 0
Test concentration [mg/kg soil dry weight] |
Nitrate-N |
||
Repl. |
MV ± Std. Dev. |
CV |
|
[mg N/kg soil dry weight] |
|||
Control |
14.2 |
14.6 ± 0.67 |
5 |
15.4 |
|||
14.3 |
|||
25.6 |
13.2 |
14.9 ± 1.51 |
10 |
16.1 |
|||
15.4 |
|||
64 |
13.7 |
13.7 ± 0.06 |
0 |
13.7 |
|||
13.6 |
|||
160 |
13.6 |
14.5 ± 0.85 |
6 |
14.6 |
|||
15.3 |
|||
400 |
14.1 |
14.0 ± 0.40 |
3 |
13.6 |
|||
14.4 |
|||
1000 |
13.6 |
13.9 ± 0.55 |
4 |
13.5 |
|||
14.5 |
Nitrate-N Contents and Nitrate-N Formation Rates - Day 7
Test concentration [mg/kg soil dry weight] |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV ± Std. Dev. |
CV |
Repl. |
MV ± Std. Dev. |
CV |
|
[mg N/kg soil dry weight] |
[mg NO3-N × (kg soil dry weight × d)-1] |
|||||
Control |
22.7 |
22.6 ± 1.11 |
5 |
1.16 |
1.14 ± 0.16 |
14 |
21.4 |
0.97 |
|||||
23.6 |
1.29 |
|||||
25.6 |
22.5 |
23.1 ± 0.85 |
4 |
1.09 |
1.18 ± 0.12 |
10 |
24.1 |
1.31 |
|||||
22.8 |
1.13 |
|||||
64 |
22.0 |
21.6 ± 0.40 |
2 |
1.19 |
1.12 ± 0.06 |
5 |
21.2 |
1.07 |
|||||
21.5 |
1.11 |
|||||
160 |
21.3 |
21.2 ± 1.30 |
6 |
0.97 |
0.96 ± 0.19 |
20 |
22.5 |
1.14 |
|||||
19.9 |
0.77 |
|||||
400 |
22.8 |
22.4 ± 0.32 |
1 |
1.26 |
1.20 ± 0.05 |
4 |
22.3 |
1.19 |
|||||
22.2 |
1.17 |
|||||
1000 |
19.1 |
19.0 ± 0.56 |
3 |
0.74 |
0.73 ± 0.08 |
11 |
18.4 |
0.64 |
|||||
19.5 |
0.80 |
Nitrate-N Contents and Nitrate-N Formation Rates - Day 14
Test concentration [mg/kg soil dry weight] |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV ± Std. Dev. |
CV |
Repl. |
MV ± Std. Dev. |
CV |
|
[mg N/kg soil dry weight] |
[mg NO3-N × (kg soil dry weight × d)-1] |
|||||
Control |
23.7 |
24.7 ± 1.00 |
4 |
0.65 |
0.72 ± 0.07 |
10 |
24.6 |
0.71 |
|||||
25.7 |
0.79 |
|||||
25.6 |
25.5 |
25.9 ± 0.67 |
3 |
0.76 |
0.79 ± 0.05 |
6 |
26.7 |
0.84 |
|||||
25.6 |
0.76 |
|||||
64 |
26.9 |
25.3 ± 1.42 |
6 |
0.94 |
0.83 ± 0.10 |
12 |
24.4 |
0.76 |
|||||
24.5 |
0.77 |
|||||
160 |
25.7 |
26.0 ± 0.74 |
3 |
0.80 |
0.82 ± 0.05 |
6 |
25.4 |
0.78 |
|||||
26.8 |
0.88 |
|||||
400 |
22.7 |
22.3 ± 0.93 |
4 |
0.62 |
0.59 ± 0.07 |
12 |
22.9 |
0.64 |
|||||
21.2 |
0.51 |
|||||
1000 |
18.6 |
18.8 ± 0.32 |
2 |
0.34 |
0.35 ± 0.02 |
6 |
18.7 |
0.34 |
|||||
19.2 |
0.38 |
Nitrate-N Contents and Nitrate-N Formation Rates - Day 28
Test concentration [mg/kg soil dry weight] |
Nitrate-N |
Nitrate-N Formation Rate |
||||
Repl. |
MV ± Std. Dev. |
CV |
Repl. |
MV ± Std. Dev. |
CV |
|
[mg N/kg soil dry weight] |
[mg NO3-N × (kg soil dry weight × d)-1] |
|||||
Control |
34.2 |
33.0 ± 1.07 |
3 |
0.70 |
0.66 ± 0.04 |
6 |
32.4 |
0.64 |
|||||
32.3 |
0.63 |
|||||
25.6 |
30.6 |
31.8 ± 1.53 |
5 |
0.56 |
0.60 ± 0.05 |
8 |
31.2 |
0.58 |
|||||
33.5 |
0.66 |
|||||
64 |
32.8 |
32.5 ± 1.04 |
3 |
0.68 |
0.67 ± 0.04 |
6 |
33.3 |
0.70 |
|||||
31.3 |
0.63 |
|||||
160 |
34.7 |
32.2 ± 2.37 |
7 |
0.72 |
0.63 ± 0.08 |
13 |
31.8 |
0.62 |
|||||
30.0 |
0.55 |
|||||
400 |
32.6 |
30.9 ± 1.60 |
5 |
0.66 |
0.60 ± 0.06 |
10 |
29.4 |
0.55 |
|||||
30.8 |
0.60 |
|||||
1000 |
33.5 |
30.4 ± 2.84 |
9 |
0.70 |
0.59 ± 0.10 |
17 |
27.9 |
0.50 |
|||||
29.9 |
0.57 |
Description of key information
In a reliable study (reliability category 1) assessing long-term effects of the supporting substance HYEQS on nitrogen transformation rate of terrestrial microorganisms, after 28 days no inhibitory effects were determined (NOEC ≥ 3.27 mmol/kg soil dry weight).
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 605.6 mg/kg soil dw
Additional information
Long-term effects on nitrogen transformation rate of terrestrial microorganisms after single exposure to the supporting substance HYEQS was assessed in a reliable (category 1) study performed according to OECD 216 and compliant to GLP over a period of 28 days. The validity criterion of the guideline was met.
HYEQS is of high similarity to C12-14 DMA in that it has essentially the same alkyl chain distribution and – like DMA category members - two methyl substituents at the amino group. The only difference is an additional hydroxyethyl substituent at the amino group rendering it a quaternary amine, being permanently positively charged. This is however a minor difference as due to protonation at environmentally relevant pH values also DMA category members are predominantly positively charged at the amino group.
HYEQS was tested at five different application rates between 25.6 and 1000 mg/kg soil dry weight. The NOEC, EC50 and EC25 for nitrogen transformation rate in soil measured as nitrate on day 7 was 400, > 1000 and 782 mg/kg soil dry weight, respectively.
The NOEC, EC50 and EC25 for nitrogen transformation rate in soil measured as nitrate on day 14 was 400, 908 and 562 mg HYEQS/kg soil dry weight, respectively.
The NOEC for nitrogen transformation rate in soil measured as nitrate on day 28 was ≥1000 mg HYEQS/kg soil dry weight: NOEC (28 d; nitrate formation rate) >= 3.27 mmol/kg soil dry weight).
In conclusion, no long-term inhibitory effects of DMA category members on the nitrogen transformation rate of terrestrial microorganisms are expected, even at high exposure concentrations. Because molar concentrations cannot be used for environmental hazard and risk assessment, in a conservative way the molecular weight of C10-DMA is used to transform the molar into mass-based concentration:
Key value for terrestrial microorganism toxicity of DMA category members: NOEC (28 d; nitrate formation rate) >= 605.6 mg/kg soil dry weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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