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EC number: 237-331-7 | CAS number: 13749-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental Starting Date: 17 May 2012, Experimental Completion Date: 03 July 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study conducted to GLP and in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not effect the quality of the relevant results.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.2110 (Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Performance of the Test:
Preparation of the Test Solutions:
Stock sample solutions were prepared in glass flasks at a nominal concentration of 1000 mg/L in the three buffer solutions. These were further diluted by a factor of 20 with the relevant buffer to give a nominal concentration of 50 mg/L. The concentration of each test solutions did not exceed the lesser of 0.01 mol/L or half the water solubility.
The test solutions were split into individual vessels for each data point.
The solutions were shielded from light whilst maintained at the test temperature.
Preliminary Test/Tier 1:
pH 4 sample solution: 50.6 mg/L
pH 7 sample solution: 50.3 mg/L
pH 9 sample solution: 50.5 mg/L
Sample solutions at pH 4, 7 and 9 were maintained at 50.0 ± 0.5 °C for a period of 120 hours.
Analysis of Sample Solutions:
The sample solutions were taken from the waterbath at various times and the pH of each solution recorded.
The concentration of the sample solution was determined by high performance liquid chromatography (HPLC).
Samples:
Aliquots were taken for analysis without any further treatment.
Standards:
Duplicate standard solutions of test item were prepared in glass double-distilled water at a nominal concentration of 50 mg/L.
Matrix blanks:
Glass double-distilled water and buffers. - Buffers:
- Buffer solution
(pH) Components Concentration
(mmol dm-3)
4 Citric acid 12
Sodium chloride 8.8
Sodium hydroxide 13.6
7 Disodium hydrogen orthophosphate (anhydrous) 6
Potassium dihydrogen orthophosphate 4
Sodium chloride 4
9 Disodium tetraborate 2
Sodium chloride 4
These solutions were subjected to ultrasonication and degassing with nitrogen to minimize dissolved oxygen content. - Estimation method (if used):
- Not used.
- Details on test conditions:
- Refer to details on sampling and analytical methods.
- Duration:
- 120 h
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.045 - 0.045 g/L
- Duration:
- 120 h
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.044 - 0.045 g/L
- Duration:
- 120 h
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.045 - 0.045 g/L
- Number of replicates:
- Duplicate.
- Positive controls:
- no
- Negative controls:
- no
- Statistical methods:
- Not specified.
- Preliminary study:
- The mean peak areas relating to the standard and sample solutions are shown in table 3.23 (please see remarks on results including tables and figures section).
- Test performance:
- Validation:
The linearity of the detector response with respect to concentration was assessed over the concentration range of 0 to 101 mg/L. This was satisfactory with a correlation coefficient (r2) of 1.000 being obtained. - Transformation products:
- not specified
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- Other kinetic parameters:
- None.
- Details on results:
- The estimated half-life at 25 °C for the test item at pH 4, 7 and 9 has been determined to be greater than 1 year.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The estimated half-life at 25 °C for the test item at pH 4, 7 and 9 has been determined to be greater than 1 year.
- Executive summary:
Method
The determination was carried out using a procedure designed to be compatible with Method C7 Abiotic Degradation, Hydrolysis as a Function of pH of Commission Regulation (EC) No 440/2008 of 30 May 2008, Method 111 of the OECD Guidelines for Testing of Chemicals, 13 April 2004 and Method 835.2110 of the OPPTS Guidelines.
The test system uses sterile buffer solutions at pH’s 4.0, 7.0 and 9.0.
Discussion
The test item was determined to be hydrolytically stable at pH 4, 7 and 9.
Conclusion
The estimated half-life at 25 °C for the test item at pH 4, 7 and 9 has been determined to be greater than 1 year.
Reference
Preliminary test/Tier 1
The mean peak areas relating to the standard and sample solutions are shown in the following table:
Table 3.23
Solution |
Mean Peak Area |
Standard 50.6 mg/L |
9.0481 x 107 |
Standard 50.3 mg/L |
8.9717 x 107 |
Initial Sample A, pH 4 |
8.8378 x 107 |
Initial Sample B, pH 4 |
8.8603 x 107 |
Initial Sample A, pH 7 |
8.7455 x 107 |
Initial Sample B, pH 7 |
8.7930 x 107 |
Initial Sample A, pH 9 |
8.9039 x 107 |
Initial Sample B, pH 9 |
8.8929 x 107 |
Standard 50.6 mg/L |
8.9763 x 107 |
Standard 50.6 mg/L |
9.0647 x 107 |
24 Hour Sample A, pH 4 |
8.8658 x 107 |
24 Hour Sample B, pH 4 |
8.8642 x 107 |
24 Hour Sample A, pH 7 |
8.7560 x 107 |
24 Hour Sample B, pH 7 |
8.7959 x 107 |
24 Hour Sample A, pH 9 |
8.9073 x 107 |
24 Hour Sample B, pH 9 |
8.8937 x 107 |
Standard 50.9 mg/L |
9.0951 x 107 |
Standard 50.4 mg/L |
9.1214 x 107 |
120 Hour Sample A, pH 4 |
8.8555 x 107 |
120 Hour Sample B, pH 4 |
8.9047 x 107 |
120 Hour Sample A, pH 7 |
8.7389 x 107 |
120 Hour Sample B, pH 7 |
8.6011 x 107 |
120 Hour Sample A, pH 9 |
8.9166 x 107 |
120 Hour Sample B, pH 9 |
8.9196 x 107 |
The test item concentrations at the given time points are shown in the following tables:
Table 3.24 pH 4 at 50°C
Time (Hours) |
Concentration (g/L) |
% of Initial |
||
A |
B |
A |
B |
|
0 |
4.48 x 10-2 |
4.49 x 10-2 |
- |
- |
24 |
4.50 x 10-2 |
4.50 x 10-2 |
100 |
100 |
120 |
4.46 x 10-2 |
4.49 x 10-2 |
99.4 |
100 |
Result: Less than 10% hydrolysis after 5 days at 50 °C, equivalent to a half-life greater than 1 year at 25 °C.
Table 3.25 pH 7 at 50 ºC
Time (Hours) |
Concentration (g/L) |
% of Initial |
||
A |
B |
A |
B |
|
0 |
4.43 x 10-2 |
4.46 x 10-2 |
- |
- |
24 |
4.45 x 10-2 |
4.47 x 10-2 |
100 |
100 |
120 |
4.40 x 10-2 |
4.33 x 10-2 |
99.0 |
97.5 |
Result: Less than 10% hydrolysis after 5 days at 50 °C, equivalent to a half-life greater than 1 year at 25 °C.
Table 3.26 pH 9 at 50 ºC
Time (Hours) |
Concentration (g/L) |
% of Initial |
||
A |
B |
A |
B |
|
0 |
4.52 x 10-2 |
4.51 x 10-2 |
- |
- |
24 |
4.53 x 10-2 |
4.52 x 10-2 |
100 |
100 |
120 |
4.49 x 10-2 |
4.49 x 10-2 |
99.6 |
99.6 |
Result: Less than 10% hydrolysis after 5 days at 50 °C, equivalent to a half-life greater than 1 year at 25 °C.
Description of key information
The estimated half-life at 25 °C for the test item at pH 4, 7 and 9 has been determined to be greater than 1 year.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 1 yr
- at the temperature of:
- 25 °C
Additional information
The determination was carried out using a procedure designed to be compatible with Method C7 Abiotic Degradation, Hydrolysis as a Function of pH of Commission Regulation (EC) No 440/2008 of 30 May 2008 and Method 111 of the OECD Guidelines for Testing of Chemicals, 13 April 2004.
The test system uses sterile buffer solutions at pH’s 4.0, 7.0 and 9.0.
Results at pH 4, pH 7 and pH 9 showed less than 10% hydrolysis after 5 days at 50 °C, equivalent to a half-life greater than 1 year at 25 °C.
As the test item was determined to be hydrolytically stable at pH 4, 7 and 9 (t½ > 1 year at 25 °C) no additional testing was performed.
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