Methanaminium, N,N,N-trimethyl-, 2,2-dimethylpropanoate (1:1)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June - December 2017

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

not specified

Specific details on test material used for the study:

Lot: 13AP016
Expiration: 1 May 2018
Purity: >99%
Appearance: White Solid

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge was obtained from a sewage plant treating predominantly domestic sewage (Nanjing Chengdong Sewage Treatment Plant) (Batch No.: AS20I 707I2I).
The sludge was washed with basal mineral salt medium (BSM) by settling the sludge in a graduated cylinder, decanting the supernatant liquid, replacing with fresh BSM, aerating, and settling. The above washing process was repeated 3 times and was aerated until use.

Duration of test (contact time):

28 d

Initial conc.:

31.6 mg/L

Based on:

COD

Initial conc.:

30 mg/L

Based on:

COD

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Duration: 28 days
Vessels: Glass BOD bottles of 5IO mL
Water: Deionised water
Measuring apparatus: Automatic respirometer (BOD meter)
Test medium: Basal Mineral Salt Medium
Controls: Inoculated sample without test substance
pH: 6.28 - 7.38
Temperature: (22±I) °C

Reference substance:

aniline

Parameter:

% degradation (O2 consumption)

Value:

55.9

Sampling time:

10 d

Parameter:

% degradation (O2 consumption)

Value:

61.8

Sampling time:

28 d

Details on results:

The initial concentration of the test substance in the reaction vessels was 31 .6 mg/L and 30.0 mg/L. The microbial inoculum concentration (mixed liquor suspended solids) was 30 mg/L.
During the test, the temperature was kept at (22±1) °C. The pH value of the content in the test container was maintained between 6.28 and 7.38. The total oxygen uptake in the inoculum blank was 26.1 mg 02/L at the end of the study, ranged 20 ~30 mg O2/L.
Biodegradation of the reference substance, aniline, reached 80.3 % at 14 days (> 60%). The difference between replicate values was less than 20% during the study. Biodegradation of inhibition control was 54.4 % at 14 days(> 25%), and the oxygen consumption by the test substance is greater than 60% of inoculum blank during the test period, indicating that there was no inhibition effect to inoculum. Thus, the test was considered valid.

Results with reference substance:

Biodegradation of the reference substance, aniline, reached 80.3 % at 14 days (> 60%).

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Under the test conditions, biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1 :1)) was 55.9 % and 61.8 % at the 10 d window and the end of the 28-day study.

Executive summary:

The ready biodegradability of Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1) was determined in a 28-day oxygen consumption test (OECD 301F) using activated sludge from a domestic waste water treatment plant as the source ofthe microbial inoculum.

The initial concentration of the test substance in the reaction vessels was 31 .6 mg/L and 30. mg/L. The microbial inoculum concentration (mixed liquor suspended solids) was 30 mg/L.

During the test, the temperature was kept at (22±1) °C. The pH value of the content in the test container was maintained between 6.28 and 7.38. The total oxygen uptake in the inoculum blank was 26.1 mg 02/L at the end of the study, ranged 20 ~30 mg O2/L.

Biodegradation of the reference substance, aniline, reached 80.3 % at 14 days (> 60%). The difference between replicate values was less than 20% during the study. Biodegradation of inhibition control was 54.4 % at 14 days(> 25%), and the oxygen consumption by the test substance is greater than 60%

of inoculum blank during the test period, indicating that there was no inhibition effect to inoculum. Thus, the test was considered valid.

Under the valid conditions, biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1 :1)) was 55.9 % and 61.8 % at the 10 d window and the end of the 28-day study.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

Obtained from aeration stage of the Seven Trent Water PLC STP at Loughborough,Leicestershire,UK, plant is treating predominantly domestic wastewater.

Duration of test (contact time):

28 d

Initial conc.:

18.8 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance:

benzoic acid, sodium salt

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

2 d

Parameter:

% degradation (CO2 evolution)

Value:

0

Sampling time:

6 d

Parameter:

% degradation (CO2 evolution)

Value:

47

Sampling time:

8 d

Parameter:

% degradation (CO2 evolution)

Value:

61

Sampling time:

10 d

Parameter:

% degradation (CO2 evolution)

Value:

93

Sampling time:

21 d

Results with reference substance:

See attached table of results

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Methanaminium N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid was found to be readily biodegradable under the consitions of OECS 301B Test.

Executive summary:

Methanaminium N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid attained 94% degradation after 28 days and satisfied the 10 -day window validation criterion where 60% degradation must be attained within 10 days of degradation exceeding 10%.

Reference 3

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Februaru - May 2018

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))

GLP compliance:

yes

Specific details on test material used for the study:

Lot: 13AP016
Purity >99%
Expiration: 2018-05-01
Appearance: White Solid

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Activated sludge, surface soil and surface water were sampled from ten sites distributed in four districts throughout Nanjing city, such as Chengdong, Chengbei, Jiangxinzhou and Jiangning. 1L of the sludge, soil and water were collected and mixed thoroughly together.
After removing floating matter, the mixture was allowed to stand and then the supernatant was filtrated through filter paper. After that the filtrate was adjusted to pH 7.0 with sodium hydroxide or phosphoric acid. Finally an appropriate volume of the filtrate was transferred to a fill-and-draw activated sludge vessel and aerated for about 23.5 h (Batch No.: IN201801041).

Duration of test (contact time):

28 d

Reference substance:

aniline

Parameter:

% degradation (O2 consumption)

Value:

92.2

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The BOD results showed that inherent biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was 92.2% after 28 days.
Based on the residue analysis, at the end of the experiment, the inherent biodegradation rate was 94.8%.

Executive summary:

Inherent biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was performed according to OECD 302C OECD: "Inherent Biodegradability: Modified MITI Test (II)".

Test solutions were prepared in an inorganic salts medium, inoculated with a number of microorganisms collected from 10 places in Nanjing city. During the test, the temperature was kept at (25±2) °C. The test was valid because the level of biodegradation of the reference substance

aniline was 56.6 % after 7 days(> 40%), and 77.3 % after 14 days(> 65%), and the recovery rate of residual amount of the test compound in the "abiotic control" is found to be more than 10% after 28 days.

The BOD results showed that inherent biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was 92.2% after 28 days.

Based on the residue analysis, at the end of the experiment, the inherent biodegradation rate was 94.8%.

Description of key information

Methanaminium N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid was found to be readily biodegradable an all tests performed.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

In an OECD 302 CO2 Evolution test, th etest item attained 94% degradation after 28 days and satisfied the 10 -day window validation criterion where 60% degradation must be attained within 10 days of degradation exceeding 10%.

In a 28-day oxygen consumption test (OECD 301F, biodegradation of the test substance was 55.9 % and 61.8 % at the 10 d window and the end of the 28-day study.

In a OECD 302C OECD: "Inherent Biodegradability: Modified MITI Test (II)", the BOD results showed that inherent biodegradation of the test substance (Methanaminium, N,N,N-trimethyl-, salt with 2,2-dimethylpropanoic acid (1: 1)) was 92.2% after 28 days. Based on the residue analysis, at the end of the experiment, the inherent biodegradation rate was 94.8%.