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EC number: 244-007-9 | CAS number: 20749-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: according to OECD TG and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
Test material
- Reference substance name:
- 8,9,10,11-tetrachloro-12H-phthaloperin-12-one
- EC Number:
- 244-007-9
- EC Name:
- 8,9,10,11-tetrachloro-12H-phthaloperin-12-one
- Cas Number:
- 20749-68-2
- Molecular formula:
- C18H6Cl4N2O
- IUPAC Name:
- 8,9,10,11-tetrachloro-12H-isoindolo[2,1-a]perimidin-12-one
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- content 99.1 %
red powder
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 2 month
- Weight at study initiation: 180-200 g
- Fasting period before study:
- Housing: before test : singly and during test in groups
- Diet ad libitum
- Water ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Humidity (%): 40-60
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- inhalation
- Type of inhalation exposure:
- nose only
- Vehicle:
- clean air
- Details on inhalation exposure:
- target concentration 5000 mg/m³
mean gravimetric concentration 2868 mg/m³
maximum technically attainable concentration
inlet air flow 28 l/min (concurrent control : air. 15 l/min)
exhaust air flow: 23.8 l/min (concurrent control: 12.8 l/min)
mean temperature 22.4.°C (concurrent control: 22.4°C
mean rel humidity 10.3 % (concurrent control: 5.1 %)
Mass Median Aerodynamic Diameter(MMAD) 4.78 µm
Aerosol Mass < 3 µm: 22 %
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- ca. 4 h
- Concentrations:
- target concentration 0 or 5000 µg/m³ (maximum technically attainable mean concentration: 2868 mg/m³)
- No. of animals per sex per dose:
- 5 rats /sex and concentration
- Control animals:
- yes
- Details on study design:
- according to the respective guideline , 2 weeks post exposure observation
To identify exposure related effects, comparisons with an appropriate vehicle control were performed. This control was exposed to an atmosphere using essentially similar exposure conditions as were used for the test substance.
body weight determination on days 1, 3, 7 and weekly thereafter
deaths were recorded
clinical signs and appearance and behavior of each rat were recorded
Necropsy was performed
and gross pathological changes were notet (if available) - Statistics:
- one-way ANOVA
The particle-size distribution was analyzed using an ANDERSEN critical orifice
cascade impactor.
Mass Median Aerodynamic Diameter (MMAD): Construct a 'Cumulative Percent
Found - Less Than Stated Particle Size' Table, calculate the total mass of test
substance collected in the cascade impactor.
Calculation of Geometric Standard Deviation (GSD): Refer to the log probability
graph used to calculate the Mass Median Aerodynamic Diameter
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 2 868 mg/m³ air (analytical)
- Exp. duration:
- 4 h
- Mortality:
- no rat died
- Clinical signs:
- other: no specific clinical signs were observed
- Body weight:
- Comparisons between the control and the exposure group did not reveal changes in body weights of toxicological significance
in body weights of no toxicological significance - Gross pathology:
- -Animals sacrificed at the end of the observation period:
No macroscopic findings - Other findings:
- no further data
Any other information on results incl. tables
Target concentration: 5000 mg/m³
Gravimetric concentration: 2868 mg/m³
MMAD: 4.78 µm
GSD 1.82
Aerosol Mass <3 µm: 22.0 %
Mass recovered: 2228 mg/m³
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information
- Executive summary:
A study on acute inhaltion toxicity of Macrolex Rot EG on rats has been conducted in accordance with OECD TG 403. A group of male and female rats was nose-only exposed to the dry powder aerosol of the test article at an actual concentration of 2868 mg/m³.. No animal died, no clinical findings and no pathotlogical changes were noted. Therefore, the results can be summarized as follows:
LC50 (rat) > 2868 mg/m³, the maximum technically attainable concentration.
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