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EC number: 219-606-3 | CAS number: 2478-10-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Dec 2004 to 16 Dec 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986 (E) (Method B)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Butanediolmonoacrylate
- Batch number: 010078 EDA 0
- Aggregate state: liquid
- Analytical purity: 99.3 corrected peak area-%
- Homogeneity: homogeneous
- Expiry date: May 2005
- Stability: guaranteed by sponsor - Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: Activated sludge from laboratory wastewater plant treating municipal sewage.
- Concentration of dry substance: 1 g/L.
- Preparation of the inoculum: inoculum was washed and brought to a concentration of 5 g/L dry substance and aerated over night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- pH:
- - pH value at test start (after correction): 7.1 - 7.2
- pH value after 30 min: 7.3 - 7.5 - Dissolved oxygen:
- - During aeration: >2.5 mg/L
- Immediately before measurement: > 6.5 mg/L - Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control), 100, 248, 496, and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: erlenmeyer-vessel
- Test volume: 250 mL
TEST MEDIUM / WATER PARAMETERS
- Synthetic medium: 8 mL/vessel 100-fold concentrated OECD medium
EFFECT PARAMETERS MEASURED: oxygen consumption
- Duration of the measurement of oxygen : 8 - 10 min - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 30 min
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Inhibition of the respiration rate was 10% in the 100 mg/L dose group and increased in a dose-dependent manner up to 48% inhibition at the dose group of 1000 mg/L. For an overview of the results, see 'Any other information on results incl. tables'.
- Results with reference substance (positive control):
- The 30-min EC50 of the reference substance was determined to be ca. 9 mg/L.
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'Any other information on materials and methods incl. tables'.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Butanediolmonoacrylate
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Source: Activated sludge from a laboratory waste water treatment plant treating municiple waste water.
- Initial biomass concentration: Concentration of activated sludge: 1 g/L dry matter - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control), 100 and 1000 mg/L
- Reference substance (positive control):
- not specified
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- At 100 mg/L, 14% of respiration inhibition was observed. No inhibition of respiration was observed at 1000 mg/L, however. See 'Any other information on results incl. tables'.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- without detailed documentation.
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Butanediolmonoacrylate
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- - Concentration of the stock solution: 12500 mg/L.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Strain: DSM 50026
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 7 h
- Test temperature:
- 297 ± 1 K
- Nominal and measured concentrations:
- - Nominal test concentrations: 0 (control), 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyers (300 mL)
- Used volume: 100 mL test media
- Shaking conditions: yes
TEST MEDIUM / WATER PARAMETERS
- Medium: AK-medium according to DIN 38412 part 8
- Stock solution: concentration 12500 mg/L
TEST SOLUTION
- 0.5 mL nutrient solution I (20 fold)
- 0.5 mL nutrient solution II (20 fold)
- 1 mL bacteria suspension
- 8 mL test substance solution - Reference substance (positive control):
- no
- Duration:
- 7 h
- Dose descriptor:
- EC10
- Effect conc.:
- 343.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 7 h
- Dose descriptor:
- EC50
- Effect conc.:
- 842.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 7 h
- Dose descriptor:
- other: EC90
- Effect conc.:
- 1 320.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
Referenceopen allclose all
Table: Respiration rate after 30 min of incubation.
Nominal Conc. [mg/l] |
O2 consumption rate [mg O2/L*h] |
Change in O2 consumption rate [%] |
0 (control) |
21* |
- |
100 |
19 |
10 |
248 |
18 |
14 |
496 |
15 |
29 |
1000 |
11 |
48 |
* mean value of blank control (3 replicates)
Table: Respiration rate after 30 min of incubation.
Nominal Conc. [mg/l] |
DOC conc. [mg/L] |
Respiration rate [mg/L x h] |
Respiration inhibition [%] |
0 |
- |
22 |
- |
100 |
58.1 |
25 |
14 |
1000 |
583.1 |
22 |
- |
Table: growth inhibition after 7 hours exposure.
Nominal Conc. [mg/l] |
Growth inhibition in % of the control |
78.125 |
108.64 |
156.25 |
100.3 |
312.5 |
92.1 |
625 |
71.01 |
1250 |
10.57 |
2500 |
0.44 |
5000 |
0.10 |
10000 |
0.10 |
Description of key information
The 30-min EC50 value is >1000 mg/L as observed in a respiration inhibition study with activated sludge treating domestic sewage.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
The toxicity of 4-hydroxyethyl acrylate to microorganisms was investigated in three different studies according to OECD guideline 209 guideline and DIN 38412 Part 8.
The study conducted most recent was in compliance with GLP regulations and performed according to OECD guidelines and therefore selected as key study. The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria (BASF, 2005). In this study, non-adapted activated sludge treating domestic sewage was exposed to nominal test substance concentrations of 0 (control), 100, 248, 496, and 1000 mg/L for 30 minutes. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition of respiration rates was first observed from the lowest test concentration of 100 mg/L (10%). The effects increased concentration-dependently reaching 48% at the highest concentration tested. Based on these findings, the 30-min EC50 and EC20 values were determined at >1000 mg/L and ca. 320 mg/L, respectively.
In a supporting study, performed according to OECD 209, domestic activated sludge was exposed to nominal test concentrations of 0 (control), 100 and 1000 mg/L for 30 minutes (BASF, 1989). At 100 mg/L, the inhibition was determined to be 14%. No inhibition of respiration was measured at 1000 mg/L, however. The 30-min EC20 value was determined to be >1000 mg/L.
In the other study, bacteria (Pseudomonas putida) were exposed to nominal test substance concentrations of 0 (control), 78.125, 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L for 7 hours (BASF, 1989). The inhibition of growth increased in a dose-dependent manner from 312.5 mg/L up to 10000 mg/L. The EC50 and EC10 values were determined to be 842.3 mg/L and 343.6 mg/L, respectively.
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