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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): not indicated
- Concentration of sludge: 750 mg/L dry substance, 30 mg/L in the test
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
112 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST CONDITIONS
- Suspended solids concentration: 30 mg/L
- The test substance was directly added to the test vessels

TEST SYSTEM
- Culturing apparatus: Sapromate
- Number of culture flasks/concentration: 7
- Test performed in closed vessels due to significant volatility of test substance: yes
- Test performed in open system: no

SAMPLING
- Sampling frequency: daily
- Sampling method: measurement of cumulative biochemical oxygen demand

CONTROL AND BLANK SYSTEM
- Inoculum blank: 2
- Abiotic sterile control: 1
- Toxicity control: 1
- Other: blank, degradation of reference substance
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
< 10
Sampling time:
28 d

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed