Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 930-389-0 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 15, 2007 - August 14, 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study without deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- , 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- , 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 2007-03-21
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): Symrose
- Substance type: technical product
- Physical state: colourless, liquid
- Storage condition of test material: ambient temperature, dark and dry
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, adapted
- Details on inoculum:
- Fresh samples of activated sludge are withdrawn on July 5th, 2007 from the sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany, which is mainly fed with municipal wastewater. The samples were washed once with mineral medium after the arrival at the laboratory and kept aerobic until use. Preparation of inoculum for exposure: aeration; Concentration of sludge: The concentration used in the test was 29.6 mg dry mass/litre (7.40 mg dry mass/250 mL).
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- ThOD/L
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Mineral medium
For the preparation of the stock solutions for mineral medium the following reagents (analytical grade) were used :
( a ) KH2PO4 8.50 g/L; K2HPO4 21.75 g/L; Na2HPO4 x 2 H2O 33.40 g/L; NH4Cl 0.50 g/L
( b ) CaCl2 x 2 H2O 36.40 g/L
( c ) MgSO4 x 7 H2O 22.50 g/L
( d ) FeCl3 x 6 H2O 0.25 g/L.
The mineral medium applied in the test contained 10 mL/L and 1 mL/L of the mineral stock solution a and b–d, respectively.
- Test temperature: 22°C
- pH: Before test start the pH values of all solutions were determined and found to be 7.4 ± 0.2. At the end of the test the pH values of all solutions were determined again.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Symrose and sodium benzoate, respectively, in concentrations of about 100 mg/L mineral medium were incubated with 29.6 mg dry mass inoculum/L mineral medium in 500 mL glass vessels at a medium volume of 250 mL.
- Number of culture flasks/concentration: Test vessels:
Test suspension: 2 vessels containing test item and inoculum
Inoculum blank: 2 vessels containing only inoculum
Abiotic control: 2 vessels containing test item and a sterilising agent
Procedural control: 2 vessels containing reference item and inoculum
Toxicity control: 2 vessels containing test item, reference item and inoculum
- Method used to create aerobic conditions: The suspension was aerated during the whole test.
- Measuring equipment: The measurement and recording of the oxygen demand was carried out continuously using a SAPROMAT respirometer (VOITH Inc.).
- Treatment of results: The Theoretical Oxygen Demand was calculated on the basis of the sum formula of the test and reference item by ThOD [g/g] = 16 * (2 C + 1/2 H + 1/2 Na – O) / Molecular weight. The ThOD values for Na-benzoate, the test item and the toxicity control were determined as follows:
ThOD(Symrose): 3.01 mg O2/mg test item
ThOD(Na-Benzoate): 1.67 mg O2/mg reference item
ThOD(toxicity control): 2.34 mg O2/mg substance mixture
The Biochemical Oxygen Demand (BOD) was calculated on the basis of the test raw data by BOD [mg/mg] = mg O2 uptake corrected by blank per mg test item. The percent degradation was calculated according to the following formula:
Dt = [(Ct –Cb) / ThOD] x 100
Dt: degradation (%) at time t;
Ct: mean oxygen consumption (mg/L) in the test suspension at time t;
Cb: mean oxygen consumption (mg/L) in the blanks at time t;
ThOD: Theoretical oxygen demand of the test suspension (mg/L).
No further details are given.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- The concentration in the test vessels with reference item (procedural control, toxicity control) was 100 mg/L mineral test medium (25 mg/250 mL).
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- The biodegradation of Symrose in the static test was found to be 0 % after 28 days. Thus, no biodegradation within a 10-day-window could be obtained and no lag phase / adaptation phase was noticeable.
No abiotic degradation of Symrose was noticeable after 28 days of incubation.
Due to a biodegradation below 60 % within the test duration and thus within a 10-day window, the test item Symrose must be identified as not readily biodegradable under the chosen test conditions.
BOD5 / COD results
- Results with reference substance:
- The biodegradation of the item mixture in the toxicity control was found to be 31 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item Symrose can be identified as non toxic in a ready biodegradability test.
The reference item sodium benzoate was degraded to 81 % within the first 14 days.
Any other information on results incl. tables
Table 1: Oxygen consumption. Cumulated consumption (mg/O2/L) after 14 days of incubation. Single and mean values of the parallel test vessels and standard deviation. Test suspension and procedural control: 100 mg/L; Toxicity control 200 mg/L
Vessel |
Test suspension |
Inoculum blank |
Abiotic control |
Precedural control |
Toxicity control |
1 |
16 |
18 |
0 |
153 |
169 |
2 |
13 |
20 |
0 |
156 |
160 |
Mean |
15 |
19 |
0 |
155 |
165 |
SD |
2 |
1 |
0 |
2 |
6 |
Table 2: Percent degradation. Degradation (%) after 14 days of incubation. Single and mean values of the parallel test vessels and standard deviation.
Vessel |
Test suspension |
Abiotic control |
Procedural control |
Toxicity control |
1 |
-1.0 |
0 |
80.5 |
32.1 |
2 |
-2.0 |
0 |
82.3 |
30.2 |
Mean |
-1.5 |
0 |
81.4 |
31.1 |
SD |
0.7 |
0 |
1.3 |
1.4 |
Table 3: Oxygen consumption. Cumulated consumption (mg/O2/L) after 28 days of incubation. Single and mean values of the parallel test vessels and standard deviation.
Vessel |
Test suspension |
Inoculum blank |
Abiotic control |
Precedural control |
Toxicity control |
1 |
23 |
23 |
1 |
159 |
189 |
2 |
17 |
24 |
1 |
174 |
166 |
Mean |
20 |
24 |
1 |
167 |
178 |
SD |
4 |
1 |
0 |
11 |
16 |
Table 4: Percent degradation. Degradation (%) after 28 days of incubation. Single and mean values of the parallel test vessels and standard deviation.
Vessel |
Test suspension |
Abiotic control |
Procedural control |
Toxicity control |
1 |
-0.2 |
0.3 |
81.4 |
35.4 |
2 |
-2.2 |
0.3 |
90.4 |
30.5 |
Mean |
-1.2 |
0.3 |
85.9 |
33.0 |
SD |
1.4 |
0.0 |
6.4 |
3.5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The biodegradation of Symrose in the static test was found to be 0 % after 28 days. Thus, no biodegradation within a 10-day-window could be obtained and no lag phase / adaptation phase was noticeable.
No abiotic degradation of Symrose was noticeable after 28 days of incubation.
The biodegradation of the item mixture in the toxicity control was found to be 31 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item Symrose can be identified as non toxic in a ready biodegradability test.
The reference item sodium benzoate was degraded to 81 % within the first 14 days.
Due to a biodegradation below 60 % within the test duration and thus within a 10-day-window, the test item Symrose must be identified as not readily biodegradable under the chosen test conditions. - Executive summary:
The test is considered valid, as
- the difference of extremes of replicate values of the removal of the test item at the end of the test was less than 20 %,
- the percentage degradation of the reference item has exceeded the pass level of 60 % by day 14,
- the oxygen uptake of the inoculum blank is < 60 mg/L in 28 days and the pH value was inside the range of 6.0 - 8.5.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.