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EC number: 600-704-3 | CAS number: 10597-60-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Study was conducted to OECD guidlines 471 Bacterial Reverse Mutation test and was in published literature rather than a offical study report
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- not specified
- Remarks:
- report was a detailed publications conducted to OECD guidelines and well conducted but not indicated if according to GLP
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
- EC Number:
- 600-704-3
- Cas Number:
- 10597-60-1
- Molecular formula:
- C8H10O3
- IUPAC Name:
- 4-(2-hydroxyethyl)benzene-1,2-diol
1
Method
- Target gene:
- Salmonella typhimurium TA 100, TA98, TA1535, and TA1537 strains and Escherichia coli WP2(pKM101))
- Metabolic activation:
- with and without
- Metabolic activation system:
- The S9 mix preparation was performed according to Ames et al.[8]. In brief, an appropriate quantity of S9 supernatant was thawed and mixed with S9 cofactor solution to result in a final protein
concentration of 0.75 mg/mL in the cultures - Test concentrations with justification for top dose:
- 5 concentrations (C5, 5 "L/plate; C4, 1.67 "L/plate; C3, 0.56 "L/plate; C2, 0.19 "L/plate; and C1, 0.06 "L/plate) with and without S9 under the directincorporation (main study) and the pre-incubation (confirmatory study) procedures
- Details on test system and experimental conditions:
- Plates were incubated for 48 h at 37 ◦C and colonies were counted. The assay was performed by triplicate along with vehicle and reference item controls. Each bacterial strain culture was mixed with the test item either with metabolic activation system mix (S9) or without metabolic activation system mix (PBS was used instead). In the direct incorporation procedure the mixture was immediately poured over a minimal agar medium plate and incubated at 37 ◦C for 48 h, whereas in the pre-incubation procedure, the mixture was incubated for 20 min at 37 ◦C prior to be poured over the minimal agar medium plate. Cytotoxicity evaluation of HT was based on the decrease in the number of revertant colonies, or a clearing or diminution of the background lawn
- Statistics:
- Statistical analysis was performed to determine the mean values of revertants/plate using a non-parametric method (Kolmogorov–Smirnov test) and the SALANAL statistical package software. Comparisons between groups were made using
Mann–Whitney U test
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- E. coli WP2 uvr A pKM 101
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Remarks:
- Main Study
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Remarks:
- Main study
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- not determinable
Applicant's summary and conclusion
- Executive summary:
Cytotoxicity evaluation of HT was performed in the S. typhimurium TA 100 strain by the direct incorporation procedure with 5 concentrations prepared by 1:3 serial dilutions starting at 50.0 µL/mL up to 0.6 µL/mL. No cytotoxic activity was observed in the bacterial system at a HT concentration of 50.0 µL/mL. None of the concentrations assayed for HT showed an increase in the R value either with or without S9 metabolic activation regardless of the procedure. No dose response for HT was observed in any of the tested bacterial strains
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