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EC number: 485-320-2 | CAS number: 221667-31-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Dec 2005 - 13 Feb 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: aeration tank of a waste water plant treating predominantly domestic sewage (Wupper area water authority, STP Odenthal); mixed population of aquatic microorganisms (activated sludge)
- Laboratory culture: No
- Pretreatment: none
- Concentration of sludge: 30 mg/L ss - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 22 ± 2°C
- Aeration of dilution water: 1 magnetic stirrer per test vessel
- pH: 7.6 (at the end of the test)
TEST SYSTEM
- Number of culture flasks/concentration: 3
- Measuring equipment: Oxi Top System (WTW)
- test volume: 250 mL
SAMPLING
- Sampling frequency: on day 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28
CONTROL AND BLANK SYSTEM
- Toxicity control: yes
- Inoculum blank: yes - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (O2 consumption)
- Value:
- 30
- Sampling time:
- 28 d
- Results with reference substance:
- The reference compound showed 83 % degradation after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 2 in "Any other information on results incl. tables".
- Interpretation of results:
- not readily biodegradable
Reference
Result for toxicity control:
Test concentration test item: 100 mg/L
Comments: The used concentrations of the test item did not show toxic effects to bacteria.
Table 1: % Biodegradation of the test item, reference compound and toxicity control.
|
|
% Biodegradation |
|||||||||
Time (days) |
2 |
6 |
8 |
12 |
14 |
16 |
20 |
22 |
26 |
28 |
|
Test item |
a1 |
0 |
0 |
0 |
22 |
24 |
26 |
29 |
29 |
32 |
30 |
a2 |
0 |
0 |
2 |
23 |
26 |
28 |
28 |
29 |
32 |
30 |
|
a3 |
0 |
0 |
0 |
23 |
24 |
26 |
28 |
29 |
30 |
30 |
|
Reference compound |
r1 |
41 |
74 |
78 |
83 |
85 |
87 |
89 |
90 |
91 |
91 |
r2 |
41 |
73 |
78 |
83 |
82 |
85 |
86 |
88 |
89 |
90 |
|
r3 |
41 |
71 |
77 |
81 |
81 |
83 |
84 |
86 |
86 |
88 |
|
Toxicity control |
t1 |
18 |
33 |
35 |
39 |
49 |
52 |
54 |
55 |
56 |
57 |
t2 |
18 |
35 |
37 |
45 |
52 |
53 |
55 |
55 |
57 |
57 |
Table 2: Validity criteria for OECD 301F.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
Difference of extremes of replicate values of the removal of the test chemical were less than 20%. |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
The reference compound attained a mean degradation of 83% after 14 days. |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
The toxicity control attained a mean degradation of 51% after 14 days. |
yes |
The oxygen uptake of the inoculum blank is normally 20-30 mg O2/L and should not be greater than 60 mg/L in 28 days. |
The oxygen uptake of the inoculum blank was 22 mg O2/L after 28 days. |
yes |
Description of key information
The substance is not readily biodegradable according to OECD criteria.
Key value for chemical safety assessment
Additional information
A GLP guideline study was performed to assess the ready biodegradability of the test substance.The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test". This test method is in all essential parts identical with OECD Guideline 301 F. The test substance shows a degradation of 30% after 28 days. Therefore the substance is considered to be "not readily biodegradable".
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