Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 457-320-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 November to 16 November 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all control and test groups
- Sampling method: no data
- Sample storage conditions before analysis: no data - Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): solution of test substance in THF coated onto aliquot of dry soil by rotary evaporation and spiked soil mixed into bulk soil
- Controls: solvent and negative
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): tetrahydrofuran (THF)
- Concentration of vehicle in test medium (stock solution and final test solution): no data
- Evaporation of vehicle before use: yes - Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: in-house from worms obtained from the University of Maryland, Queenstown, Md USA
- Age at test initiation (mean and range, SD): adult (with clitellum)
- Weight at test initiation (mean and range, SD): range 0.52 to 0.58 g
ACCLIMATION
- Acclimation period: 24 h
- Acclimation conditions (same as test or not): yes
- Health during acclimation (any mortality observed): none - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- none
- Test temperature:
- At initiation: 19.7 to 20.3oC
At termination: 19.0 to 19.2oC - pH:
- At test initiation: 6.4 to 6.6
At termination: 7.9 - Moisture:
- At initiation: 32.9 to 33.3%
At termination: 31.1 to 32.1% - Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 1 L glass beaker
- Amount of soil or substrate: 750 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): quartz sand (approx. 70%); kaolin clay (approx. 20%); sphagnum peat (approx. 10%) plus CaCO3 (1%)
- Organic carbon (%): 3.9
- Maximum water holding capacity (in % dry weight): no data
- Pretreatment of soil: none
- Storage (condition, duration): none
- Stability and homogeneity of test material in the medium:
- stability: mean recovery at day 14 - 103%, 96% and 96% at low, middle and high concentrations
- homogeneity: mean ± SD (CV) at lowest conc - 59.8 ± 0.839 (1.4%); at highest conc - 944 ± 27.6 (2.93%)
OTHER TEST CONDITIONS
- Photoperiod: continuous illumination
- Light intensity: 400-800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : day 0 - body weight, burrowing behaviour, mortality; day 7 and 14 - mortality, behaviour and structural abnormalities; day 14 - body weight
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: x2
- Justification for using less concentrations than requested by guideline: not applicable - Nominal and measured concentrations:
- nominal: 62.5, 125, 250, 500 and 1000 mg/kg dry weight soil
measured: 59.8, 118, 253, 484 and 944 mg/kg dry weight soil - Reference substance (positive control):
- yes
- Remarks:
- but in a separate study
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Details on results:
- - Mortality at end of exposure period: No mortalities in the controls, or treatment groups other than one earthworm not found (presumed dead) in the 250 mg/kg (nominal) treatment group, which was not considered treatment related.
- Changes in body weight of live adults at end of exposure period: weight loss varied from 0.12 g (negative control) to 0.06 g (1000 mg/kg group). This slight loss of body weight in the control and treatment groups was not unexpected since the earthworms were not fed during the test.
- No. of offspring produced: no data
- No. of unhatched cocoons: no data
- Morphological abnormalities: none
- Behavioural abnormalities: none
- Other biological observations: none - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: LC50 - 24.5 mg a.i/kg dry soil for chloroacetamide - Reported statistics and error estimates:
- Body weight data - Dunnett's 2-tailed test of means (alpha=0.05) after data tested for homogeneity of variance and normal distribution.
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP study conducted according to OECD guideline 207, no treatment-related mortality, or adverse effects on body weight change, appearance and behaviour were seen in earthworms (Eisenia fetida) exposed to EC#457-320-2 in artificial soil for 14 days at up to nominal concentrations of 1000 mg/kg dry weight soil (considered the NOEC in this study). The 14-day LC50 is therefore greater than 1000 mg EC# 457-320-2/kg dry wt soil.
- Executive summary:
In a GLP study conducted according to OECD guideline 207, the acute toxicity to earthworms (Eisenia fetida) of EC# 457-320-2 (with a molybdenum content of about 10%) has been determined following exposure for 14 days in artificial soil. Animals were exposed to nominal concentrations of 62.5, 125, 250, 500 and 1000 mg EC# 457-320-2/kg dry wt of soil and examined for mortality, body weight change, appearance and behaviour after 7 and 14 days. The potential uptake of the test material over the course of the study was also assessed.
In the treatment groups, only one death was seen on day 7 in an earthworm exposed to 250 mg EC# 457-320-2/kg dry wt soil, which was not considered to be treatment-related. No mortality was seen in the control groups during the study. No adverse effects were seen on behaviour or appearance in any control or treatment groups during the study. A reduction in mean body weight was seen at day 14 in all groups, but this was not unexpected as animals were not fed during the experimental period. Analysis of the earthworms on day 14 of the study showed that tissue levels of molybdenum were below the limit of quantitation of the analytical method.
No treatment-related mortality, or adverse effects on body weight change, appearance and behaviour were seen in earthworms (Eisenia fetida) exposed to EC# 457-320-2 in artificial soil for 14 days at up to nominal concentrations of 1000 mg/kg dry wt soil; considered the no-observed-effect concentration (NOEC) in this study. The 14-day LC50 is therefore greater than 1000 mg/kg dry wt soil. Earthworms did not accumulate EC# 457-320-2, measured as molybdenum content, over the study period.
Reference
Description of key information
The 14-day NOEC in earthworms was 1000 mg/kg dry wt soil; the 14-day LC50 was >1000 mg/kg dry wt soil.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 1 000 mg/kg soil dw
Additional information
One toxicity to soil macroorganisms except arthropods study is available for EC# 457-320-2: a 14-day acute study in earthworms, conducted to GLP and in accordance with OECD Test Guideline 207. The earthworms were exposed to artificial soil spiked with EC# 457-320-2 test material (with a molybdenum content of approximately 10%) at nominal concentrations of 0, 62.5, 125, 250, 500 and 1000 mg EC# 457-320-2/kg dry weight of soil.
There were no treatment-related deaths or effects on appearance, behaviour or body weight. Analysis of the earthworms on day 14 of the study showed that tissue levels of molybdenum were below the limit of quantitation of the analytical method. The NOEC was concluded to be 1000 mg/kg dry wt soil; the LC50 was >1000 mg/kg dry wt soil.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.