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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tricyclo[8.2.2.24,7]hexadeca-4,6,10,12,13,15-hexaene, 5,11,13,15-tetramethyl-
- Cas Number:
- 205825-52-1
- Molecular formula:
- C20H24
- IUPAC Name:
- Tricyclo[8.2.2.24,7]hexadeca-4,6,10,12,13,15-hexaene, 5,11,13,15-tetramethyl-
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Batch no.: 350321
Expiry date: February 26, 2024
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Remarks:
- Due to low water solubility of the test item, a solvent (acetonitrile) was used as carrier to add the test item in test medium
- Details on test solutions:
- The test concentration was chosen based on the water solubility value determined in a GLP study (35 µg/L).
Starting from water solubility data we have performed solubility preliminary tests in ecotoxicological matrix (reconstituted water) and following method of solutions preparation described below: 50 µg test item/L was assessed as maximum soluble tested concentration.
It corresponded to 49.8 µg PARYFREE® DIMER/L (calculated considering active ingredient content reported in the CoA, 99.6%).
Due to low water solubility of the test item, a solvent (acetonitrile) was used as carrier to add the test item in test medium. An additional control containing the solvent at the same concentration as used in the test solution was included in the test design.
Prior to the test start, a 500.0 mg test item/L stock solution was prepared by direct weighting 0.0053 g of test item into 10 mL acetonitrile solvent. The stock solution was clear and transparent with the test item totally solubilized. It was stored in a refrigerator and used again to prepare the fresh test item solution after 24 hours.
Starting from this stock solution, the test solution at 50 µg test item/L was prepared transferring 50 µL of stock solution into 500 mL of reconstituted water (according to the OECD No. 23 that recommends a maximum solvent concentration equal to 0.1 ml/L).
Test solution was clear and transparent, it was mixed for 24 hours in order to obtain the maximum test item solubilization. After stirring, it was always clear and transparent with the test item totally solubilized.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- For the test concentration, for the negative control and positive control, group of 20 daphnids were used divided into four replicates of five daphnids each
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 19.94 – 20.03°C
- pH:
- negative control: 7.40 - 7.81
positive control: 7.68 – 7.81
test item solution: 7.55 – 7.83 - Dissolved oxygen:
- negative control: 6.71 – 6.92 mg O2/L
positive control: 6.98 – 7.48 mg O2/L
test item solutions: 6.96 – 7.94 mg O2/L - Nominal and measured concentrations:
- 50.0 µg test item/L, corresponding to 49.8 µg PARYFREE® DIMER/L (calculated considering active ingredient content reported in the CoA, 99.6%).
- Reference substance (positive control):
- yes
- Remarks:
- reconstituted water without test item but with acetonitrile solvent
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 50 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 27.15 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 27.15 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Details on results:
- Since all analytical recoveries were not in the range 80 % - 120 % of the nominal concentrations, the biological results were referred to the time-weighted arithmetic mean of PARYFREE® DIMER measured concentrations, as recommended by OECD Guidance Document on testing of difficult substances No. 23, 2019 (Table 4).
No immobilization was observed and no daphnid was trapped on the test water surface or showed other signs of disease or stress.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- No immobilization was observed and no daphnid was trapped on the test water surface or showed other signs of disease or stress.
The results, calculated in terms of nominal test item concentration, were as follows:
EC50 > 50 ug/l
NOEC = 50 ug/l
The results, calculated in terms of time-weighted arithmetic mean of active ingredient measured concentrations, were as follows:
EC50 > 27.15 ug/l
EC50 = 27.15 ug/l
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