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EC number: 292-960-4 | CAS number: 91031-57-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Refer to read across justification provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other:
- Remarks:
- Source: CAS 85049-37-2, Oleon, 1995, activated sludge
- Conclusions:
- No acute toxic effects on microorganisms were found up to the limit of water solubility (OECD 209, activated sludge, RA CAS 85049-37-2).
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 Jul 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Each test solution was prepared by dissolving a certain amount of test substance in a mixture of 16 mL of the synthetic sewage and 200 mL of mixed liquor. The mixture was then diluted to 500 mL with deionised water. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no, activated sludge was obtained from the waste water treatment plant of Bocholt /Germany), which predominantly treats domestic sewage.
- Preparation of inoculum for exposure: The sludge was sieved through 0.5 mm sieve in order to remove large particles. A small amount of the mixed liquor is dried and weighed. From this result, a calculated amount of mixed liquor was suspended in water to obtain an activated sludge with mixed liquor suspended solids level of 4 g/L
- Initial biomass concentration: 1.6 g/L in test solution - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.0 - 20.3 °C
- pH:
- Test end: 7.71-7.83
- Nominal and measured concentrations:
- Nominal: 6.5, 13.7, 26.0, 50.1 and 100.2 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Material: borosilicate glass; Size: 1000 mL; Fill volume: 500 mL
- Aeration: constant supply with clean, oil-free air
- No. of vessels per concentration: 1
- No. of vessels per control: 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: aerated tap water
EFFECT PARAMETERS MEASURED : Respiration rate inhibition was recorded after 3 h test duration. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - No inhibition of the respiration rate was observed at the highest test concentration of 102 mg/L. Instead, a 16.2% stimulation was observed.
- Effect concentrations exceeding solubility of substance in test medium.
- The two control respiration rates are within 15% of each other. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50(3h) is in the range of 5 to 30 mg/L (21.7 mg/L) - Reported statistics and error estimates:
- Logit model of Chou and Chou (1985), determining the median effect
Referenceopen allclose all
Table 1: Respiration rates of activated sludge in control and % inhibition at different test concentrations:
Test substance [mg/L] |
Respiration rate [mg O2/L*h] |
Inhibition [%] |
Control 1 |
18.02 |
|
Control 2 |
17.09 |
|
6.5 |
19.91 |
- 13.4 |
13.7 |
21.54 |
- 22.7 |
26 |
19.49 |
- 11.0 |
50.1 |
21.89 |
- 24.7 |
100.2 |
20.39 |
- 16.2 |
Description of key information
No effects up to the limit of water solubility (OECD 209, activated sludge); read-across
Key value for chemical safety assessment
Additional information
There is no study available assessing the toxicity of the target substance Fatty acids, C16-18, isononyl esters (CAS 91031-57-1) to microorganisms. Therefore, read-across to the structurally related source substance Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters (CAS 85049-37-2) was conducted in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5. The source substance is considered a suitable representative for the assessment of the toxicity of the target substance to microorganisms. A detailed read-across justification is provided in IUCLID section 13.
In the available study, the acute toxicity of Fatty acids, C16-18 and C18-unsatd., 2-ethylhexyl esters on activated sludge was tested according to OECD 209 under GLP conditions. After an incubation time of 3 hours, no inhibition of the respiration rate was observed within the tested concentration range of 6.5 to 100.2 mg/L (nominal), resulting in an EC50(3h) value of >100 mg/L.
Based on the structural and chemical similarity of the target and source substance, the target substance is expected to exhibit a similar ecotoxicological profile. Therefore, it can be concluded that Fatty acids, C16-18, isononyl esters (CAS 91031-57-1) will not exhibit toxic effects to microorganisms up to the limit of water solubility.
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