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EC number: 229-761-9 | CAS number: 6711-48-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
N'-[3-(dimethylamino)propyl]-N,N-dimethylpropane-1,3-diamine was negative in an Ames test, test guideline OECD 471, GLP (BASF AG 1998).
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (only 2-aminoanthracene as positive control with metabolic activation)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- his operon and trp operon
- Species / strain / cell type:
- other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100 and E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- Experiment 1: standard plate test 1: 0; 20; 100; 500; 2500 and 5000 µg/plate (with and without S9-mix); all strains
Experiment 2: standard plate test 2: 2000, 3000, 4000, 5000, 6000 µg/plate (with and without S9-mix); (TA100, E.coli WP2 uvrA)
Experiment 3: preincubation test : 0; 20; 100; 500; 2500 and 5000 µg/plate (with and without S9-mix); all strains - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
- Untreated negative controls:
- yes
- Remarks:
- sterility control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water control
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: (+ S9): 2-aminoanthracene, 2-AA (all strains); (- S9): N-methyl-N'-nitro-N-nitroso-guanidine, MNNG (TA 100, TA 1535); 4-nitro-o-phenylendiamine, NOPD (TA 98); 9-aminoacridine, AAC (TA 1537); N-ethyl-N'-nitro-N-nitrosoguanidin, ENNG (E.coli WP2 uvrA)
- Remarks:
- 2-AA: 2 µg (TA100, TA 98, TA 1537, TA 1535), 60 µg (E. coli WP2 uvrA); MNNG: 5 µg; NOPD: 10 µg; AAC: 100 µg; ENNG: 10 µg; solvent for all positive control substances: DMSO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: standard plate test (first experiment), preincubation test (second experiment)
DURATION
- Preincubation period: 20 min (only preincubation test)
- Exposure duration: 48 - 72 h (in the dark, 37 °C)
NUMBER OF REPLICATIONS: 3 plates/concentration/experiment; 3 3experiments
DETERMINATION OF CYTOTOXICITY
- Method: reduction in the number of revertants or a clearing of the bacterial background lawn, reduction of the titer.
The titer is generally determined only in the experimental parts with S9 mix both for the negative controls (vehicle only) and for the two highest doses in all experiments. - Evaluation criteria:
- The test chemical is considered positive in this assay if the following criteria are met: A dose-related and reproducible increase in the number of revertant colonies, i.e. about doubling of the spontaneous mutation rate in at least one tester strain either without S-9 mix or after adding a metabolizing system.
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A weak bacteriotoxic effect in the standard plate test at 6000 µg/plate (TA 100). In the preincubation assay bacteriotoxicity was found depending on the strain and test conditions at doses >= 500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A weak bacteriotoxic effect in the standard plate test at 6000 µg/plate. In the preincubation assay bacteriotoxicity was found at doses >= 2500 µg/plate.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: no test substance precipitation was found - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative
Reference
Table 1: Test results of experiment 1 (plate incorporation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA 100 |
TA1535 |
WP2 uvrA |
TA98 |
TA1537 |
||
– |
0 |
116 ± 13 |
14 ± 2 |
30 ± 1 |
27 ± 5 |
9 ± 2 |
– |
20 |
130 ± 32 |
13 ± 3 |
39 ± 3 |
25 ± 7 |
8 ± 2 |
– |
100 |
153 ± 14 |
16 ± 5 |
41 ± 7 |
32 ± 5 |
8 ± 1 |
– |
500 |
161 ± 23 |
20 ± 4 |
37 ± 8 |
22 ± 7 |
10 ± 2 |
– |
2500 |
153 ± 13 |
18 ± 2 |
48 ± 4 |
25 ± 3 |
9 ± 3 |
– |
5000 |
179 ± 3 |
21 ± 9 |
44 ± 7 |
27 ± 8 |
9 ± 2 |
Positive controls, –S9 |
Name |
MNNG |
MNNG |
ENNG |
NOPD |
AAC |
Concentrations (μg/plate) |
5 |
5 |
10 |
10 |
100 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1223 ± 268 |
1070 ± 278 |
698 ± 36 |
982 ± 16 |
575 ± 6 |
|
+ |
0 |
119 ± 13 |
15 ± 3 |
46 ± 5 |
42 ± 4 |
9 ± 2 |
+ |
20 |
171 ± 24 |
16 ± 5 |
56 ± 5 |
40 ± 8 |
11 ± 0 |
+ |
100 |
143 ± 23 |
15 ± 5 |
39 ± 4 |
40 ± 13 |
10 ± 3 |
+ |
500 |
138 ± 22 |
19 ± 5 |
44 ± 6 |
43 ± 6 |
12 ± 3 |
+ |
2500 |
154 ± 10 |
22 ± 3 |
54 ± 13 |
40 ± 3 |
7 ± 1 |
+ |
5000 |
147 ± 17 |
21 ± 3 |
57 ± 7 |
33 ± 4 |
9 ± 3 |
Positive controls, +S9 |
Name |
2AA |
2AA |
2AA |
2AA |
2AA |
Concentrations (μg/plate) |
2.5 |
2.5 |
60 |
2.5 |
2.5 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1297 ± 57 |
112 ± 3 |
327 ± 37 |
577 ± 33 |
214 ± 21 |
ENNG = N-ethyl-N-nitro-N-nitrosoguanidine
MNNG = N-methyl-N-nitro-N-nitrosoguanidine
NOPD = 4-nitro-o-phenylendiamine
AAC = 9-aminoacridine
2AA = 2-Aminoanthracene
B = reduced background growth
Table 2: Test results of experiment 2 (plate incorporation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
|
|
|
|
||
TA 100 |
WP2 uvrA |
||
– |
0 |
117 ± 5 |
35 ± 2 |
– |
2000 |
105 ± 5 |
38 ± 3 |
– |
3000 |
120 ± 13 |
41 ± 2 |
– |
4000 |
108 ± 11 |
38 ± 7 |
– |
5000 |
90 ± 10 |
45 ± 4 |
– |
6000 |
148 ± 20 |
31 ± 9 |
Positive controls, –S9 |
Name |
MNNG |
ENNG |
Concentrations (μg/plate) |
5 |
10 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1307 ± 138 |
505 ± 6 |
|
+ |
0 |
126 ± 28 |
36 ± 6 |
+ |
2000 |
136 ± 11 |
32 ± 3 |
+ |
3000 |
142 ± 5 |
32 ± 4 |
+ |
4000 |
119 ± 19 |
36 ± 4 |
+ |
5000 |
97 ± 11 |
24 ± 3 |
+ |
6000 |
0B |
0B |
Positive controls, +S9 |
Name |
2AA |
2AA |
Concentrations (μg/plate) |
2.5 |
60 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1182 ± 66 |
220 ± 34 |
ENNG = N-ethyl-N-nitro-N-nitrosoguanidine
MNNG = N-methyl-N-nitro-N-nitrosoguanidine
NOPD = 4-nitro-o-phenylendiamine
AAC = 9-aminoacridine
2AA = 2-Aminoanthracene
B = reduced background growth
Table 3: Test results of experiment 3 (preincubation)
With or without S9-Mix |
Test substance concentration (μg/plate) |
Mean number of revertant colonies per plate (average of 3 plates ± Standard deviation) |
||||
Base-pair substitution type |
Frameshift type |
|||||
TA 100 |
TA1535 |
WP2 uvrA |
TA98 |
TA1537 |
||
– |
0 |
141 ± 10 |
22 ± 4 |
33 ± 4 |
31 ± 3 |
8 ± 2 |
– |
20 |
117 ± 4 |
18 ± 1 |
36 ± 2 |
28 ± 7 |
9 ± 2 |
– |
100 |
113 ± 13 |
19 ± 1 |
34 ± 4 |
30 ± 2 |
8 ± 1 |
– |
500 |
94 ± 8 |
7 ± 2 |
34 ± 5 |
16 ± 2 |
8 ± 1 |
– |
2500 |
0B |
0B |
14 ± 3 |
0B |
0B |
– |
5000 |
0B |
0B |
0B |
0B |
0B |
Positive controls, –S9 |
Name |
MNNG |
MNNG |
ENNG |
NOPD |
AAC |
Concentrations (μg/plate) |
5 |
5 |
10 |
10 |
100 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
1104 ± 47 |
789 ± 44 |
521 ± 33 |
1087 ± 70 |
408 ± 42 |
|
+ |
0 |
138 ± 10 |
23 ± 0 |
41 ± 7 |
46 ± 4 |
9 ± 1 |
+ |
20 |
124 ± 10 |
20 ± 3 |
40 ± 3 |
41 ± 2 |
11 ± 3 |
+ |
100 |
103 ± 1 |
21 ± 3 |
40 ± 2 |
44 ± 9 |
13 ± 6 |
+ |
500 |
88 ± 8 |
21 ± 3 |
35 ± 3 |
36 ± 9 |
11 ± 5 |
+ |
2500 |
102 ± 5 |
13 ± 3 |
50 ± 4 |
30 ± 7 |
8 ± 2 |
+ |
5000 |
43 ± 9B |
0B |
18 ± 2 |
0B |
7 ± 1 |
Positive controls, +S9 |
Name |
2AA |
2AA |
2AA |
2AA |
2AA |
Concentrations (μg/plate) |
2.5 |
2.5 |
60 |
2.5 |
2.5 |
|
Mean No. of colonies/plate (average of 3 ± SD) |
983 ± 31 |
121 ± 9 |
227 ± 14 |
574 ± 54 |
103 ± 11 |
ENNG = N-ethyl-N-nitro-N-nitrosoguanidine
MNNG = N-methyl-N-nitro-N-nitrosoguanidine
NOPD = 4-nitro-o-phenylendiamine
AAC = 9-aminoacridine
2AA = 2-Aminoanthracene
B = reduced background growth
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Bacterial reverse mutation assay:
BASF (1998) performed an Ames (plate incorporation assay and preincubation test) test with S. typhimurium strains TA1535, TA1537, TA98 and TA100 and E. coli WP2 with and without metabolic activation in presence of Bis (3-dimethylaminopropyl) amin (Bis-DMAPA).
Following test concentrations were applied: 0, 20, 100, 500, 2500 and 5000 µg/plate (in triplicate) with and without metabolic activation (S9 -mix) using the standard plate incorporation assay for all strains, and 2000, 3000, 4000, 5000 and 6000 µg/plate (with and without S9 -mix) for S. typhimurium strain TA100 and E. coli WP2 uvrA. Solvent control, negative control and positive controls were run in triplicate. Following cytotoxic effects were observed in Salmonella strains: A weak bacteriotoxic effect in the standard plate test at 6000 µg/plate (TA 100). In the preincubation assay bacteriotoxicity was found depending on the strain and test conditions at doses >= 500 µg/plate. Following cytotoxic effects were observed in E. coli WP2 uvrA: toxic effect in the standard plate test at 6000 µg/plate. In the preincubation assay bacteriotoxicity was found at doses >= 2500 µg/plate. Solvent, negative and positive controls were valid.
Justification for classification or non-classification
Classification,Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. As a result the substance is not considered to be classified for genetic toxicity under Regulation (EC) No. 1272/2008, as amended for the third time in Directive (EC 618/2012).
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