Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 835-183-3 | CAS number: 83420-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- GLP compliance:
- yes
- Type of method:
- HPLC estimation method
- Media:
- soil/sewage sludge
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material:
190701
- Expiration date of the lot/batch:
24 July 2021
- Purity:
>90%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Room Temperature
- Stability under storage conditions:
Stable - Radiolabelling:
- no
- Test temperature:
- Room temperature
- Details on study design: HPLC method:
- INSTRUMENT HPLC-DAD (Agilent 1100)
COLUMN Luna CN (150mmx4.6mmx5.0um)
COLUMN TEMPERATURE 25°C
AUTOSAMPLER TEMP 20°C
WAVELENGTH 210 nm (scan 190-400)
MOBILE PHASE A% = 45%10 mM citrate buffer at pH 6.0
B% = 55% methanol
FLOW 1.0 mL/min
MOBILE PHASE Signal, Bw (nm): 210, 4
Reference, Bw (nm): 360, 100
INJECTION VOLUME 5.0 pl
RUN TIME 8 min - Details on test conditions:
- TEST CONDITIONS
- Buffer:
About 5.88 g of Sodium citrate tribasic dihydrate was quantitatively weighed and transferred into a beaker contained about 1500 mL of HP water.
- pH:
The pH was measured and corrected with HCI 1M until the value was 6. The solution was brought to 2000 mL with HP water.
- Suspended solids concentration:
not applicable
TEST SYSTEM
- Type, size and further details on reaction vessel:
not applicable
- Water filtered (i.e. yes/no; type of size of filter used, if any):
not applicable
- Amount of soil/sediment/sludge and water per treatment (if simulation test):
not applicable
- Soil/sediment/sludge-water ratio (if simulation test):
not applicable
- Number of reaction vessels/concentration:
13
- Measuring equipment:
Agilent Series 1100 High Performance Liquid Chromatograph (HPLC) equipped with an Agilent Series 1100 variable wavelength UV-DAD detector, a binary pump, an autosampler and a data station with Chemstation - internal code LV-B1162.
- Test performed in closed vessels due to significant volatility of test substance:
not applicable
- Test performed in open system:
not applicable
- Method of preparation of test solution:
Mobile Phase/Solvent Mixture
450 mL of Citrate Buffer pH 6 was mixed with 550 mL of Methanol.
Sodium Nitrate stock solution (conc. = 1.0 mg/mL)
About 50 mg of Sodium Nitrate reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution A).
Sodium Nitrate solution (conc. = 0.025 mg/mL)
0.4 mL of Sodium Nitrate stock solution was diluted to 20 mL with solvent mixture.
Benzamide stock solution (conc. = 1.0 mg/mL)
About 50 mg of Benzamide reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution B).
Benzamide solution (conc. = 0.025 mg/mL)
0.5 mL of Benzamide stock solution was diluted to 20 mL with solvent mixture
Acetanilide stock solution (conc. = 1.0 mg/mL)
About 50 mg of Acetanilide reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution C).
Acetanilide solution (conc. = 0.015 maq/mL)
0.3 mL of Acetanilide stock solution was diluted to 20 mL with solvent mixture.
2,5-Dichloroaniline stock solution (conc. = 1.0 mg/mL)
About 50 mg of 2,5-Dichloroaniline reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution D).
2,5-Dichloroaniline solution (conc. = 0.0075 maq/mL)
0.15 mL of 2,5-Dichloroaniline stock solution was diluted to 20 mL with solvent mixture.
Benzoic acid Phenylester stock solution (conc. = 1.0 mg/mL)
About 50 mg of Benzoic acid Phenylester reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution E).
Benzoic acid Phenylester solution (conc. = 0.025 mg/mL)
0.5 mL of Benzoic acid Phenylester stock solution was diluted to 20 mL with solvent mixture.
Fenthion stock solution (conc. = 1.0 mg/mL)
About 10 mg of Fenthion reference standard was exactly weighed into a 10 mL volumetric flask and brought to volume with Methanol (Solution F).
Fenthion solution (conc. = 0.025 mg/mL)
0.5 mL of Fenthion stock solution was diluted to 20 mL with solvent mixture.
Phenanthrene stock solution (conc. = 1.0 mg/mL)
About 50 mg of Phenanthrene reference standard was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol (Solution G).
Phenanthrene solution (conc. = 0.025 ma/mL)
0.25 mL of Phenanthrene stock solution was diluted to 20 mL with solvent mixture.
The solution was brought to volume with solvent mixture.
Test item stock solution (conc. = 1 mg/mL - double preparation)
About 50 mg of test item was exactly weighed into a 50 mL volumetric flask and brought to volume with Methanol.
Test item solution + sodium nitrate (conc. = 0.2 mg/mL - double preparation)
2 mL of this solution and 0.5 mL of solution A was diluted to 10 mL with solvent mixture.
Test item solution (conc. = 0.2 mg/mL)
2 mL of this solution was diluted to 10 mL with solvent mixture.
- Are the residues from the adsorption phase used for desorption:
no - Key result
- Sample No.:
- #1
- Type:
- log Koc
- Value:
- > 4.09 dimensionless
- pH:
- 6
- Temp.:
- 20 °C
- Matrix:
- Soils
- Remarks on result:
- not determinable because of methodological limitations
- Key result
- Sample No.:
- #2
- Type:
- log Koc
- Value:
- > 4.35 dimensionless
- pH:
- 6
- Temp.:
- 20 °C
- Matrix:
- sewage sludge
- Remarks on result:
- not determinable because of methodological limitations
- Details on results (HPLC method):
- The results are reported below :
The calibration reference standards, consist of 6 reference substances of known log Kcc: Acetanilide, Benzamide, 2,5-Dichloroaniline, Benzoic acid Phenylester, Fenthion and Phenanthrene.
The standards chosen had a log Ko, for soils between 1.26 — 4.09 and a log Ko, for sewage sludge between 1.52 — 4.35.
Calibration line of the reference standardsfor soils
Linear Correlation:
slope 3,9830
intercept 2,1717
correlation 0,9771
R2 0,9547
Calibration line of the reference standards for sewage sludge
Linear Correlation:
slope 4,1077
intercept 2,4246
correlation 0,9822
R2 0,9647
Sample peak didn’t elute in the conditions prescribed by OECD 121 and log Koc cannot be estimate. It can be supposed that log K,, on soils and sewage sludge are higher than logkK,, on soils and sewage sludge of phenanthrene (the most retained standard). - Transformation products:
- no
- Validity criteria fulfilled:
- yes
- Executive summary:
The aim of the study was to estimate Adsorption Coefficient (Koc) on Soil and on Sewage Sludge by high performanceliquid chromatography (HPLC) method according to OECD 121 oftest item “N4,N4'- hexane-1 ,6-diylbis[N-butyl-6-chloro-N,N'-bis(2,2,6,6-tetramethylpiperidin-4-yl)-1,3,5-triazine-2,4 diamine].
The adsorption coefficient is defined as the ratio between the concentration of the substance in the soil/sludge and the concentration of the substance in the aqueous phase at adsorption equilibrium. The adsorption coefficient normalised to the organic carbon content of the soil (Koc) is a useful indicator of the binding capacity of a chemical on organic matter of soil and sewage sludge and allows comparisons to be made between different chemicals.
The adsorption coefficient was estimated by the HPLC method .
The Test Item didn’t elute with the chromatographic conditions prescribed by the guidelines OECD 121 (methanol/0.01M buffer citrate pH 6.0, 55/45%v/v) and it was retained in the column, so it cannot be estimated the logKoc of the test item.
Since the most retained reference standard, used in the calibration curve, was phenanthrene (logKoc on soils: 4.09 - logKoc on sewage sludge: 4.35), it can be supposed that, at pH 6, logKoc on soils for the test item is higher than 4.09 and logKoc on sewage sludge is higher than 4.35.
Reference
Description of key information
The adsorption coefficient was estimated by the HPLC method .
The Test Item didn’t elute with the chromatographic conditions prescribed by the guidelines OECD 121 (methanol/0.01M buffer citrate pH 6.0, 55/45%v/v) and it was retained in the column, so it cannot be estimated the logKoc of the test item.
Since the most retained reference standard, used in the calibration curve, was phenanthrene (logKoc on soils: 4.09 - logKoc on sewage sludge: 4.35), it can be supposedthat, at pH 6, logKoc on soils for the test item is higher than 4.09 and logKoc on sewage sludge is higher than 4.35.
Key value for chemical safety assessment
- Koc at 20 °C:
- 4.35
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.