N-(4-(methoxybenzyl))-N,N-dimethylanilinium hexafluoroantimonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 days

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

other: ISO 9439

Version / remarks:

(1999)

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: Merck, Darmstadt, Germany
- Purity, including information on contaminants, isomers, etc.: 99.0%

RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity:
- Specific activity:
- Locations of the label:
- Expiration date of radiochemical substance:

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Stable
- Stability and homogeneity of the test material in the vehicle/solvent under test conditions (e.g. in the exposure medium) and during storage: In freezer (<= -10deg C) in the dark
- Stability in the medium: No data
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium: No data
- Reactivity of the test material with the incubation material used (e.g. plastic ware): No data

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding):
- Preliminary purification step (if any):
- Final concentration of a dissolved solid, stock liquid or gel:
- Final preparation of a solid (e.g. stock crystals ground to fine powder using a mortar and pestle): Since Ammonium hexafluoroantimonate was not sufficiently soluble to allow preparation of an aqueous solution at a concentration of 1 g/l, weighed amounts of Ammonium hexafluoroantimonate were added to the test bottles (2 litres) containing medium with microbial organisms and mineral components (test substance bottle A: 60.6 mg; test substance bottle B: 60.7 mg and toxicity control bottle: 60.5 mg). To this end, 10 ml of MiMi-RO water was added to each weighing bottle containing the test substance. After vigorous shaking the resulting suspension was added quantitatively to the test medium. The test solutions were continuously stirred during the test, to ensure optimal contact between the test substance and the test organisms.

FORM AS APPLIED IN THE TEST (if different from that of starting material)
- Specify the relevant form characteristics if different from those in the starting material, such as state of aggregation, shape of particles or particle size distribution:

INFORMATION ON NANOMATERIALS
- Chemical Composition:
- Density:
- Particle size & distribution:
- Specific surface area:
- Isoelectric point:
- Dissolution (rate):

TYPE OF BIOCIDE/PESTICIDE FORMULATION (if applicable)
- Description of the formulation, e.g. formulated product for foliar application; formulated product soil application; solution in organic solvent for soil application; formulated product seed treatment; solution in organic solvent seed treatment:

OTHER SPECIFICS
- Other relevant information needed for characterising the tested material, e.g. if radiolabelled, adjustment of pH, osmolality and precipitate in the culture medium to which the test chemical is added:

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Duration of test (contact time):

ca. 28 d

Initial conc.:

12 mg/L

Based on:

TOC

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: Milli RO water
- Additional substrate:
- Solubilising agent (type and concentration if used): Milli RO water
- Test temperature: 22-23 degrees C
- pH: 7.6
- pH adjusted: yes FROM 7.7.-7.8 using 0.1mol/HCL
- CEC (meq/100 g):
- Aeration of dilution water: Yes
- Suspended solids concentration: 4.8g/l
- Continuous darkness: no
- Other:

TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
- Method used to create aerobic conditions:
- Method used to create anaerobic conditions:
- Measuring equipment:
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used:
- Other:

SAMPLING
- Sampling frequency:
- Sampling method:
- Sterility check if applicable:
- Sample storage before analysis:
- Other:

CONTROL AND BLANK SYSTEM
- Inoculum blank:
- Abiotic sterile control:
- Toxicity control:
- Other:

STATISTICAL METHODS:

Reference substance:

other: Sodium acetate

Key result

Parameter:

% degradation (CO2 evolution)

Value:

ca. 3 - ca. 6

Sampling time:

29 d

Key result

Parameter:

ThOD

Value:

ca. ca. 1.47 other: mg CO2/mg

Results with reference substance:

yes sodium acetate degraded at 78% after 29 days

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Test substance not readily biodegradable.

Description of key information

Under the conditions of this study it was found that the test substance was not biodegradable.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater