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EC number: 855-027-8 | CAS number: 2409921-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From March 14, 2019 to July 15, 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Vehicle:
- yes
- Remarks:
- Deionized water, acetonitrile, ammonium formate, reconstitute water.
- Details on test solutions:
- Deionized water: Prepared from the Arium® Pro ultrapure water system
Acetonitrile: J. T. Baker; liquid chromatography level; 99.9% purity
Ammonium formate: SIGMA, 99.0% purity
Reconstitute water: Method of preparation was shown in Table 1. Expiration date: No longer than six months. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Source: National Chiayi University Freshwater Biological Resource Center
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 19-21°C
- Nominal and measured concentrations:
- In limit test: nominal concentration was 100 mg/L
- Details on test conditions:
- - Test volume: 30 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- Calibration curve: The analytical results of the test solution of each concentration at 0.10, 1.00, 10.00, 50.00 and 100.00 µg/mL showed that the linear equation of the calibration curve was y = 48.877383x - 0.626373, while R = 1.000.
LOQ(limit of quantification): Added the test article into deionized water and made its final concentration was 0.10 µg/mL. The results showed that the signal-to-noise ratio (S/N) ranged between 21.058 ~ 28.903.
Recovery rate: The test article was added into deionized water and made its final concentration was 0.10 and 100.00 µg/mL, respectively. The results showed that the average recovery rates were 111.60%, 101.13% at 0.10 and 100.00 µg/mL, respectively.
Stability test: The average recovery rate were respectively 100.50% and 101.22% at refrigeration. After stored at room temperature (RT, 25 ± 2℃) for 1 day. The average recovery rate were respectively 109.00% and 101.47%.
Quantitative analysis: The results indicated that the concentrations of samples from the test group of the day 2 were 149.58%~154.05% higher than the initial level. - Validity criteria fulfilled:
- yes
- Conclusions:
- According to OECD 202 test method, the 48-hr EC50 of CR SB33 was >100 mg/L.
- Executive summary:
This test using the procedures outlined in the SuperLab Study Plan for MZ6-181200027 which is based on the SOP for the OECD 202 (SOPE-505) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CR SB33 show that all validity criteria were met. Under the conditions of this test, the 48-hr EC50 of CR SB33 was >100 mg/L.
Reference
Range-finding test: The results showed that the number of immobilized daphnids in control and test groups after the end of the test was 0, and thus the percentages immobilized was 0.00%.
Limit test:
1. The results showed that, at the end of the test, the number of immobilised daphnids in 100.00 mg/L nominal concentration group was 0 (Table 1), percentages immobilised was 0.00%, and EC50 was thus greater than 100.00mg/L nominal concentrations (Table 2).
2. The analytical results of test article concentration (Table 3) showed that test article was undetected in concrol group at 0 and 48 hours of the test. In test group, the test article concentration determined was at the range of 108.055~108.695 µg/mL at 0 hour of the test and was at the range of 166.059~166.883 µg/mL at 48 hours.
Table 1. The numbers of immobilized daphnids for the limit test
Group (mg/L) | Time (hours) | Numbers of immobilised activitya | Number of discoloration | Numbers of trapping at surface of water | |
Control1 | 0.00 | 24 | 0 | 0 | 0 |
Control2 | 0.00 | 24 | 0 | 0 | 0 |
Control3 | 0.00 | 24 | 0 | 0 | 0 |
Control4 | 0.00 | 24 | 0 | 0 | 0 |
Test1 | 100.00 | 24 | 0 | 0 | 0 |
Test2 | 100.00 | 24 | 0 | 0 | 0 |
Test3 | 100.00 | 24 | 0 | 0 | 0 |
Test4 | 100.00 | 24 | 0 | 0 | 0 |
Control1 | 0.00 | 48 | 0 | 0 | 0 |
Control2 | 0.00 | 48 | 0 | 0 | 0 |
Control3 | 0.00 | 48 | 0 | 0 | 0 |
Control4 | 0.00 | 48 | 0 | 0 | 0 |
Test1 | 100.00 | 48 | 0 | 0 | 0 |
Test2 | 100.00 | 48 | 0 | 0 | 0 |
Test3 | 100.00 | 48 | 0 | 0 | 0 |
Test4 | 100.00 | 48 | 0 | 0 | 0 |
aDeath of daphnids was also viewed as one of immobilisation responses.
Table 2. The percentages immobilized and EC50 for the limit test
Group (mg/L) |
Percentages immobilised (%) 24 hours 48 hours |
EC50(mg/L) |
||
Control1 |
0.00 |
0.00 |
0.00 |
- |
Control2 |
0.00 |
0.00 |
0.00 |
- |
Control3 |
0.00 |
0.00 |
0.00 |
- |
Control4 |
0.00 |
0.00 |
0.00 |
- |
Test1 |
100.00 |
0.00 |
0.00 |
>100.00 |
Test2 |
100.00 |
0.00 |
0.00 |
>100.00 |
Test3 |
100.00 |
0.00 |
0.00 |
>100.00 |
Test4 |
100.00 |
0.00 |
0.00 |
>100.00 |
Table 3: Analytical results of concentrations in test solution of limit test
Sampling time | Sampling date | Analysis date | Nominal concentration (µg/mL) | Sample (test iquid) concentration (µg/mL) |
0 hour | 2019/3/26 | 2019/3/26 |
0.000 |
0.000 |
0.000 |
0.000 |
|||
0.000 |
0.000 |
|||
0.000 |
0.000 |
|||
100.000 |
108.332 |
|||
100.000 |
108.695 |
|||
100.000 |
108.462 |
|||
100.000 |
108.055 |
|||
48 hours |
2019/3/28 |
2019/3/28 |
0.000 |
0.000 |
0.000 |
0.000 |
|||
0.000 |
0.000 |
|||
0.000 |
0.000 |
|||
100.000 |
166.883 |
|||
100.000 |
162.585 |
|||
100.000 |
166.216 |
|||
100.000 |
164.059 |
Table 4. Temperature, dissolved oxygen and pH value of the limit test
Group (mg/L) |
Time |
Item |
Mean±SD |
|
Control |
0.00 |
0 hour |
Temperature (℃) |
21.0 |
Dissolved oxygen (mg/L) |
7.94±0.02 |
|||
pH value |
7.19 ±0.01 |
|||
48 hours |
Temperature (℃) |
21.0 |
||
Dissolved oxygen (mg/L) |
7.96±0.08 |
|||
pH value |
7.32±0.03 |
|||
Test |
100.00 |
0 hour |
Temperature (℃) |
21.0 |
Dissolved oxygen (mg/L) |
8.06±0.03 |
|||
pH value |
7.27±0.01 |
|||
48 hours |
Temperature (℃) |
21.0 |
||
Dissolved oxygen (mg/L) |
8.05±0.09 |
|||
pH value |
7.48±0.04 |
aTemperature referred to the air temperature in the ambient environment measured when testing
Description of key information
The 48-hr EC50 of CR SB33 was >100 mg/L for Daphnia magna (OECD TG202).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
In both range-finding and limit test, not more than 10 % of the Daphnia were immobilised in the control. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Under the conditions of this test, the 48-hr EC50 of CR SB33 was >100 mg/L.
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