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EC number: 250-528-2 | CAS number: 31242-17-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 21 July 2014-08 August 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken from the limit concentration and the control according to the schedule below. In addition, the filter used to prepare the limit concentration was retained for possible analysis of the residue.
Frequency: at t=0 h, t=24 h and t=96 h
Volume: 2.0 ml
Storage: Samples were stored in a freezer until analysis. - Vehicle:
- no
- Details on test solutions:
- The batch of EXP1313100 tested was a brown solid that was not completely soluble in test medium at the loading rates initially prepared.
All test solutions were prepared separately. Various loading rates of the test substance were added to the test medium applying two days of magnetic stirring in order to reach maximum solubility. The resulting aqueous mixtures were filtered through a 0.45 µm membrane filter (Whatman) where after the clear and colourless Water Accommodated Fractions (WAFs) were used for testing. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
Mean length Range-finding test: 3.1 ± 0.3 cm
Final test: 3.3 ± 0.3 cm
Mean weight1 Range-finding test: 0.82 ± 0.26 g
Final test: 1.11 ± 0.22 g
Characteristics: F1 from a single parent-pair bred in UV-treated water.
Reason for selection: This system has been selected as an internationally accepted species.
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same as the test
- Feeding: Daily with pelleted fish food
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- between 22 °C and 23 °C
- pH:
- between 7.5 and 8.1
- Dissolved oxygen:
- between 5.8 and 8.8 mg/l
- Nominal and measured concentrations:
- Nominal concentrations:Control and a WAF prepared at a loading rate of 100 mg/L.
Measured concentration: Analysis of the samples taken at the start of the limit test showed a measured concentration of 0.0045 mg/L in the WAF prepared at a loading rate of 100 mg/L. This concentration slightly decreased to 72% of initial at the end of the test. The average exposure concentration was calculated to be 0.0038 mg/L. - Details on test conditions:
- Test type: Static
Test vessels: 20 litres, all-glass, containing 20 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
Number of fish: 7 per concentration
Loading: 0.39 g fish/litre, i.e. 7 fish per 20 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: Aeration was introduced after 1 day of exposure.
Feeding: No feeding from 24 hours prior to the test and during the total test period
TEST MEDIUM / WATER PARAMETERS
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O: 211.5 mg/L
MgSO4.7H2O: 88.8 mg/L
NaHCO3: 46.7 mg/L
KCl : 4.2 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :LC50 t=24, 48, 72, 96H
TEST CONCENTRATIONS
- Final study: control and a WAF prepared at a loading rate of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP)
- Duration:
- 24 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Behavioral abnormalities:
- Mortality of control: No mortality in control
- Abnormal responses: no abnormalities were observed
- Effect concentrations exceeding solubility of substance in test medium: yes - Results with reference substance (positive control):
- Under the conditions of the present test PCP induced no lethal effects in carp at 0.10 mg/L. The
96h-LC50 for carp exposed to PCP was 0.24 mg/L. This effect was already reached within 48 hours of exposure. - Reported statistics and error estimates:
- Calculation of LC50
The 24, 48, 72 and 96h-EC50 values could not be determined because the observed effects were below 50%. - Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was recorded at any of the loading rates of EXP1313100 tested.
The 96h-LL*50 was above a loading rate of 100 mg EXP1313100 per litre. The concentration of EXP1313100 in the WAF prepared at a loading rate of 100 mg/L was determined to be 0.0038 mg/L based on the average exposure concentration.
*LL = lethal loading rate
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 21 July 2014-08 August 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 7346-1 (Determination of the Acute Lethal Toxicity of Substances to a Freshwater Fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)] - Part 1: Static Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for analysis were taken from the limit concentration and the control according to the schedule below. In addition, the filter used to prepare the limit concentration was retained for possible analysis of the residue.
Frequency: at t=0 h, t=24 h and t=96 h
Volume: 2.0 ml
Storage: Samples were stored in a freezer until analysis. - Vehicle:
- no
- Details on test solutions:
- The batch of EXP1313100 tested was a brown solid that was not completely soluble in test medium at the loading rates initially prepared.
All test solutions were prepared separately. Various loading rates of the test substance were added to the test medium applying two days of magnetic stirring in order to reach maximum solubility. The resulting aqueous mixtures were filtered through a 0.45 µm membrane filter (Whatman) where after the clear and colourless Water Accommodated Fractions (WAFs) were used for testing. - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- Species: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758
Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands.
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
Mean length Range-finding test: 3.1 ± 0.3 cm
Final test: 3.3 ± 0.3 cm
Mean weight1 Range-finding test: 0.82 ± 0.26 g
Final test: 1.11 ± 0.22 g
Characteristics: F1 from a single parent-pair bred in UV-treated water.
Reason for selection: This system has been selected as an internationally accepted species.
ACCLIMATION
- Acclimation period: At least 12 days after delivery
- Acclimation conditions (same as test or not): same as the test
- Feeding: Daily with pelleted fish food
- Health during acclimation (any mortality observed): In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg/l expressed as CaCO3
- Test temperature:
- between 22 °C and 23 °C
- pH:
- between 7.5 and 8.1
- Dissolved oxygen:
- between 5.8 and 8.8 mg/l
- Nominal and measured concentrations:
- Nominal concentrations:Control and a WAF prepared at a loading rate of 100 mg/L.
Measured concentration: Analysis of the samples taken at the start of the limit test showed a measured concentration of 0.0045 mg/L in the WAF prepared at a loading rate of 100 mg/L. This concentration slightly decreased to 72% of initial at the end of the test. The average exposure concentration was calculated to be 0.0038 mg/L. - Details on test conditions:
- Test type: Static
Test vessels: 20 litres, all-glass, containing 20 litres of test solution
Test medium: Adjusted ISO medium with a hardness of 180 mg CaCO3 per litre and a pH of 7.7 ± 0.3
Number of fish: 7 per concentration
Loading: 0.39 g fish/litre, i.e. 7 fish per 20 litres of test medium
Illumination: 16 hours photoperiod daily
Aeration: Aeration was introduced after 1 day of exposure.
Feeding: No feeding from 24 hours prior to the test and during the total test period
TEST MEDIUM / WATER PARAMETERS
Medium: Adjusted ISO medium, formulated using RO-water (tap-water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O: 211.5 mg/L
MgSO4.7H2O: 88.8 mg/L
NaHCO3: 46.7 mg/L
KCl : 4.2 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 hours photoperiod daily
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :LC50 t=24, 48, 72, 96H
TEST CONCENTRATIONS
- Final study: control and a WAF prepared at a loading rate of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- pentachlorophenol (PCP)
- Duration:
- 24 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 0.004 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Behavioral abnormalities:
- Mortality of control: No mortality in control
- Abnormal responses: no abnormalities were observed
- Effect concentrations exceeding solubility of substance in test medium: yes - Results with reference substance (positive control):
- Under the conditions of the present test PCP induced no lethal effects in carp at 0.10 mg/L. The
96h-LC50 for carp exposed to PCP was 0.24 mg/L. This effect was already reached within 48 hours of exposure. - Reported statistics and error estimates:
- Calculation of LC50
The 24, 48, 72 and 96h-EC50 values could not be determined because the observed effects were below 50%. - Validity criteria fulfilled:
- yes
- Conclusions:
- No mortality was recorded at any of the loading rates of EXP1313100 tested.
The 96h-LL*50 was above a loading rate of 100 mg EXP1313100 per litre. The concentration of EXP1313100 in the WAF prepared at a loading rate of 100 mg/L was determined to be 0.0038 mg/L based on the average exposure concentration.
*LL = lethal loading rate
Referenceopen allclose all
Description of key information
No mortality was recorded at any of the loading rates. The 96h-LL50 was above a loading rate of 100 mg/l.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.