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EC number: 474-080-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 11.11. 2019 – 7.1. 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 020
- Report date:
- 2020
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 474-080-4
- EC Name:
- -
- Cas Number:
- 76801-93-9
- Molecular formula:
- C14H18I3N3O6
- IUPAC Name:
- 5-amino-N1,N3-bis(2,3-dihydroxypropyl)-2,4,6-triiodobenzene-1,3-dicarboxamide
- Reference substance name:
- Unknown impurities
- Molecular formula:
- Not available
- IUPAC Name:
- Unknown impurities
- Test material form:
- solid
Constituent 1
impurity 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 00104016
- Expiration date of the lot/batch: 12/2020
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in cool place. Keep container tightly closed in a dry and well-ventilated place.
- Stability under test conditions: Stable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- INOCULUM
The inoculum contained a mixed population of microorganisms obtained from the secondary effluent of the waste water treatment plant of Pardubice processing predominantly municipal sewage.
PREPARATION OF THE INOCULUM
The fresh collected waste water was filtered through paper filter and aerated till using.
JUSTIFICATION OF THE TEST SYSTEM
The inoculum preparation is in conformity with the recommendations of the test guideline. - Duration of test (contact time):
- > 0 - <= 28 d
Initial test substance concentration
- Initial conc.:
- 2.5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- PREPARATION OF SOLUTIONS
Inoculated medium: The volume of waste water for the inoculation of mineral medium was chosen 2.1 mL per 1 L of medium. 30 L aerated mineral medium was prepared for the test and before the beginning of the test it was inoculated with 63 mL of modified waste water. The pH value of medium: 7.4.
Test item: The stock solution of the test item was prepared in concentration 0.1001 g·L-1 of deionized water. From this stock solution 150 mL (25 mL·L-1) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L. The solution prepared in this way contained 2.5 mg·L-1 of the test item. The pH value of solution: 7.4.
Reference item: The stock solution of sodium benzoate was prepared in concentration 1.0000 g·L-1 of deionized water. From this solution the 15 mL (2.5 mL·L-1) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L. The solution prepared in this way contained 2.5 mg·L-1 of sodium benzoate. The pH value of solution: 7.5.
Toxicity test: The solution was prepared by dosing 100 mL (25 mL·L-1) of stock solution of the test item and 10 mL (2.5 mL·L-1) of stock solution of the reference item into inoculated medium and completed with this medium to the volume of 4 L. The mixture prepared in this way contained 2.5 mg·L-1 of the test item and 2.5 mg·L-1 of sodium benzoate.
Control (blank) determination: The inoculated mineral medium without test item was used for the blank determination.
TEST CONDITIONS
Temperature: 20 +/- 1.0 °C
Illumination: no illumination, in the dark
Exposition time: 28 days
TEST PROCEDURE
The number of bottles for individual series:
Test item series: 2 x 9 bottles with test item and inoculum
Reference item series: 2 x 9 bottles with reference item and inoculum
Blank series: 2 x 9 bottles with inoculated medium only
Toxicity test series: 2 x 5 bottles with test item, reference item and inoculum for toxicity test
Series for nitrification determination: 1 x 9 bottles with test item and inoculum
The prepared dosing solutions for each series were filled in two parallel bottle replicates (except series for nitrification determination). The bottles were placed into thermostat.
MEASUREMENT
The first two bottles from each series were immediately submitted to analysis for dissolved oxygen (zero-time) and other bottles were placed in the thermostat.
On the 3rd, 7th, 10th, 14th, 17th, 21st, 24th and 28th day of the test, two bottles (parallel replicate) from the test item series, from the reference item series and from the blank series were taken off and the same determination was performed.
On the 3rd, 7th, 10th, 14th day of the test, two bottles from the toxicity test series were taken off and the same determination was performed.
The analysis of dissolved oxygen was performed electrochemically using oximeter device (oximeter WTW OXI 730).
On the 3rd, 7th, 10th, 14th, 17th, 21st, 24th and 28th day of the test, one bottle from the series for nitrification determination was taken off and stored in freezer. The determinations of nitrite-N concentration and nitrate-N concentration were carried out by ion chromatography at one time at the end of the test.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- ThOD of the reference item: 1.665 mg·mg-1
Any other information on results incl. tables
RESULTS EVALUATION
The oxygen consumption and percentage of the biodegradation of the test item for each test interval and each bottle were calculated.
In time intervals when the increase of oxidized nitrogen forms was detected (in interval 17th– 28thday),the correction of oxygen consumption for nitrification was carried out.
The evaluation of the reference item and of the test and reference item mixture (toxicity test) was done by the same way.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In this 28-day study of ready biological degradability the degradation of 0 % of the test item, ATIBA-A, was attained in the end of study.
- Executive summary:
The test item, ATIBA-A, was tested for the ready biological degradability in Closed Bottle Test.
Test performance
The test was performed according to:
Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008
OECD Test Guideline No. 301 D - Closed Bottle Test. Adopted July 17, 1992
The test item was sufficiently soluble in used mineral medium so the dosage from the stock solution was carried out.
Sodium benzoate was used as the reference item.
In parallel to the main test the toxicity test was performed.
Because the test item contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out.
The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7 – 8 at the beginning of the test.
The calculations of biological degradation are based on ThOD (theoretical oxygen demand) values of the test and reference item.
Validity of the test
The prescribed validity criteria in the test were fulfilled.
The test item was not inhibiting for the used inoculum.
Since all criteria of acceptability were met, this study is considered to be valid.
Test results
In this 28-day study of ready biological degradability the degradation of 0 % of the test item, ATIBA-A, was attained in the end of study.
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