Reaction mass of 2-dodecylhexadecyl methacrylate and 2-Tetradecyloctadecyl methacrylate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 07 October 2019 to 10 October 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

The first definitive test did not correlate with the rangefinding test. The definitive test was repeated, and the first and second definitive tests correlate. The second definitive test met all relevant validity criteria.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accommodated Fractions

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: hatched from ephippia sourced from Eco Cheshire Solutions

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Remarks on exposure duration:

Observations of test animals were carried out at 48h and at 24h for the definitive and reference tests.

Test temperature:

20°C ± 2°C

pH:

7 - 8

Dissolved oxygen:

8.49 – 8.66 mg/L

Nominal and measured concentrations:

Nominal concentrations (2nd definitive test): 180, 320, 560, 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL capacity borosilicate glass crystallising dishes
- Type: closed
- Volume of solution: 50 mL
- Type of flow-through: static
- No. of organisms per vessel: 10 animals per vessel
- No. of vessels per concentration (replicates): 2 vessels per concentration
- No. of vessels per control (replicates): 2 vessels per control
- No. of vessels per vehicle control (replicates): 2 vessels per control

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO reconstituted water (as specified in OECD 202)
- Intervals of water quality measurement: Carried out at each concentration at 0h and 48h

EFFECT PARAMETERS MEASURED: Immobilisation

OTHER TEST CONDITIONS
- Photoperiod: 16h light / 8h dark

RANGE-FINDING STUDY
- Test concentrations: 1,000, 100, 10 and 1 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

928 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 5%

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Relevant effect levels: Yes
- Limit test: No
- Dose-response test: Yes
- 24h EC50: 1.4 mg/L

Nominal concentration (mg/l)	Number dead immobile at 24h		Number dead/immobile at test termination (48h)		Total exposed
	Replicate a	Replicate b	Replicate a	Replicate b
100	0	0	0	3	20
180	0	0	2	1	20
320	0	0	3	2	20
560	0	0	5	3	20
1000	7	5	2	7	20
1800	10	8	10	9	20
3200	10	9	10	9	19
5600	10	10	10	10	20
10000	10	10	10	10	20

Validity criteria fulfilled:

yes

Conclusions:

The 48-hour EC50 was 1,120 mg/L and the 48-hour NOEC 320 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Executive summary:

The short-term toxicity of Reaction mass of 2-Propenoic acid, 2-methyl-, 2-dodecylhexadecyl ester and 2-Propenoic acid, 2-methyl-, 2-tetradecyloctadecyl ester (EC 946-978-0) to aquatic invertebrates was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

Since the substance was poorly water soluble, Water Accomodated Fractions (WAF) were prepared. Results are expressed based on the initial loading of test substance required to produce the effects.

The species was Daphnia magna.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 180, 320, 560, 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 48-hour EC50 was 1,120 mg/L and the 48-hour NOEC 320 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Description of key information

The short-term toxicity of Reaction mass of 2-Propenoic acid, 2-methyl-, 2-dodecylhexadecyl ester and 2-Propenoic acid, 2-methyl-, 2-tetradecyloctadecyl ester (EC 946-978-0) to aquatic invertebrates was determined during a GLP-compliant study performed according to the OECD Testing Guideline 202.

The species was Daphnia magna.

A range-finding test was performed to determine the concentrations to be used during the definitive test.

As a result of this range-finding test, concentrations of 180, 320, 560, 1,000, 1,800, 3,200, 5,600 and 10,000 mg/L were used during the definitive test.

The 48-hour EC50 was 1,120 mg/L and the 48-hour NOEC 320 mg/L. The substance did not meet the criteria for classification as toxic to the aquatic environment according to Regulation (EC) N°1272/2008.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 120 mg/L