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EC number: 619-409-6 | CAS number: 99208-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Februare 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- In the blank control vessels, 16 mL nutrient solution was mixed with 234 mL water. The positive control vessels and the treatments were prepared by putting the appropriate amount of positive control solution respectively test item into the respective test vessel, adding 16 mL nutrient solution and water to reach a total volume of 250 mL. Then, 250 mL inoculum was added in 5 minutes intervals and the mixtures were aerated.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Tap water
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- After 3 hours, the content of the first vessel was poured in a 250 mL narrow-neck bottle and the respiration rate was determined by measurement of the O2-concentration over a period of max. 5 minutes. The following vessels were measured likewise in 5 minutes intervals.
- Hardness:
- 1.18 mmol/L
- Test temperature:
- 18.2-20.3 oC
- pH:
- 8.12
- Conductivity:
- 270 μS/cm
- Nominal and measured concentrations:
- 1,10,100,1000 mg/L nominal concentrations
- Details on test conditions:
- Duration 3 hours
Replicates
Positive Control 1 replicate/concentration
Test item 1 replicate/concentration (lower concentrations) 5 replicates/concentration (highest concentration 1000 mg/L)
Blank Control 2 replicates before and 2 after measuring positive control and test item, respectively
Water tap water, see annex 2, page 24
Aeration purified air, using Pasteur pipettes,
Feeding nutrient solution, 16 mL/vessel
Temperature 18.2 – 20.3 °C - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (1,3-Dichloro-5-hydroxybenzene, C6H4Cl2O, CAS-No. 591-35-5) was used as positive control. A stock solution in deionised water containing 500 mg/L was freshly prepared for the experiment.
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- 3h-EC50 = 9.5 mg/L
- Reported statistics and error estimates:
- For the treatments with the test item concentrations 1000 mg/L, it was tested whether the differences between treatment and blank control were significant. For this determination, the values of the O2 consumption were used.
In order to select a suitable test for significance, it was checked whether equality of variance was given.
As equality of variance is given, the t-test is used. - Validity criteria fulfilled:
- yes
- Remarks:
- A value of 9.5 mg/L for positive control lies within the recommended range of 2 – 25 mg/L. The coefficient of variation of oxygen uptake rate in blank control replicates of 4%was determined is less than 30% at the end of the test.
- Conclusions:
- One experiment was performed.
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal con-centration. For the treatment 1000 mg/L, 5 replicates were used; for the other treatments, 1 replicate each.
Because no significant inhibition was observed, no additional experiment had to be per-formed. - Executive summary:
The test item was tested using 4 concentrations ranging from 1000 to 1 mg/L nominal concentration. Toxicity to microorganisms is greater then 1000 mg/L,
Reference
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.