Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 306-764-4 | CAS number: 97404-02-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30Oct2018 to 28Feb2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-[(4-aminophenyl)azo]-1,3-dimethyl-1H-imidazolium chloride
- EC Number:
- 306-764-4
- EC Name:
- 2-[(4-aminophenyl)azo]-1,3-dimethyl-1H-imidazolium chloride
- Cas Number:
- 97404-02-9
- Molecular formula:
- C11H14N5.Cl
- IUPAC Name:
- 2-[(1E)-2-(4-aminophenyl)diazen-1-yl]-1,3-dimethyl-2,3-dihydro-1H-imidazol-1-ium chloride
- Test material form:
- solid
- Details on test material:
- Batch 1075126
Constituent 1
- Specific details on test material used for the study:
- Identification: 2-[(4-aminophenyl)azo]-1,3-dimethyl-1Himidazolium chloride
Batch (Lot) Number: 1075126
Expiry date: 24 July 2022 (expiry date)
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for possible analysis were taken from the stock solution, all test concentrations and the control according to the schedule below.
Frequency at t=0 h and t=48 h
Volume 1.8 mL from the approximate centre of the test vessels
Storage Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Additionally, reserve samples of 1.8 mL were taken for possible analysis. If not used, these samples were stored in a freezer (≤-15°C) for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
Source In-house laboratory culture with a known history.
Reason for selection This system has been selected as an internationally accepted invertebrate species.
Validity of batch Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%1, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Characteristics Daphnia, less than 24 hours old, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- other: M7, as prescribed by Dr. Elendt-Schneider (Elendt, B.-P., 1990: Selenium deficiency in Crustacea. An ultrastructural approach to antennal damage in Daphnia magna Straus. Protoplasma 154, 25-33).
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- In alignment with OECD 202
- Post exposure observation period:
- N/A
Test conditions
- Hardness:
- The hardness of test medium expressed as CaCO3: 180 mg/L
- pH:
- 8.0 to 8.2 throughout duration of the test.
- Dissolved oxygen:
- 9.2-9.7 mg/L throughout duration of the test.
- Salinity:
- N/A
- Nominal and measured concentrations:
- Limit/Range test
0.1; 0.117-0.124
1.0; not analysed
10; 10.8-11.3
100; not tested
Final test
Nominal (mg/L); Measured (mg/L)
0.46;0.443-0.453
1.0; 0.986-1.01
2.2; 2.25-2.32
4.6; 4.50-4.52
10; 9.97-10.3 - Details on test conditions:
- Test duration; 48 hours
Test type; Static
Test vessels; 60 mL, all-glass.
Test medium; The following salts (analytical grade) were added to tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
The hardness of test medium expressed as CaCO3: 180 mg/L with a pH between 6 and 9.
Number of daphnids; 20 per concentration
Loading; 5 per vessel containing 50 mL of test solution.
Light; A daily photoperiod of 16 hours.
Feeding; No feeding
Aeration; No aeration of the test solutions was applied.
Introduction of daphnids; 41 minutes after preparation of the test solutions. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Reported statistics and error estimates:
- The 48h-EC50 for Daphnia magna exposed to 2-[(4-aminophenyl)azo]-1,3-dimethyl-1H-imidazolium chloride was 3.2 mg/L based on analytically confirmed nominal concentrations (95% confidence interval between 2.2 and 4.6 mg/L).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 48h-EC50 for Daphnia magna exposed to 2-[(4-aminophenyl)azo]-1,3- dimethyl-1H-imidazolium chloride was 3.2 mg/L based on analytically confirmed nominal concentrations (95% confidence interval between 2.2 and 4.6 mg/L).
- Executive summary:
The objective of the study was to evaluate 2-[(4-aminophenyl)azo]-1,3-dimethyl-1Himidazolium chloride for its ability to generate acute toxic effects on the mobility of Daphnia magna during an exposure period of 48 hours and, if possible, to determine the EC50 at 24 and 48 hours of exposure.
The study procedures described in this report were based on the OECD guideline No. 202, 2004.
The batch of 2-[(4-aminophenyl)azo]-1,3-dimethyl-1H-imidazolium chloride tested was a brown powder with a purity of 99.85% and completely soluble in test medium at the concentrations tested.
A final test was performed based on the results of a preceding combined limit/range-finding test. Twenty daphnids per group (5 per replicate, quadruplicate) were exposed to an untreated control and to nominal concentrations of 0.46, 1.0, 2.2, 4.6 and 10 mg/L. The total exposure period was 48 hours and samples for analytical confirmation of exposure concentrations were taken at the start and at the end of the test.
Samples taken from all test concentrations and the control were analysed. The measured concentrations were at the level of the nominal concentrations throughout the exposure period, i.e. at 96-106% relative to the nominal concentrations at the start and at the end of the test. The effect parameters were thus based on analytically confirmed nominal concentrations.
No immobility was observed in the control and the three lowest test concentrations throughout the exposure period, while 55% of daphnids exposed to 4.6 and 10 mg/L were immobilized at 24 hours of exposure and 100% of daphnids exposed to 4.6 and 10 mg/L were immobilized at the end of the test.
The study met the acceptability criteria prescribed by the study plan and was considered valid.
In conclusion, the 48h-EC50 for Daphnia magna exposed to 2-[(4-aminophenyl)azo]-1,3 -dimethyl-1H-imidazolium chloride was 3.2 mg/L based on analytically confirmed nominal concentrations (95% confidence interval between 2.2 and 4.6 mg/L).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.