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EC number: 216-231-7 | CAS number: 1530-48-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study plan dated : 24. Apr. 2019 and experimental completion date 02. May. 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Allyltributylphosphonium chloride
- EC Number:
- 216-231-7
- EC Name:
- Allyltributylphosphonium chloride
- Cas Number:
- 1530-48-9
- Molecular formula:
- C15H32P.Cl
- IUPAC Name:
- tributyl(prop-2-en-1-yl)phosphanium chloride
- Reference substance name:
- Tetrahydrothiophene 1,1-dioxide
- EC Number:
- 204-783-1
- EC Name:
- Tetrahydrothiophene 1,1-dioxide
- Cas Number:
- 126-33-0
- Molecular formula:
- C4H8O2S
- IUPAC Name:
- tetrahydrothiophene 1,1-dioxide
- Reference substance name:
- Tributyl-1-propen-1-yl-phosphonium chloride
- Molecular formula:
- C15H32P.Cl
- IUPAC Name:
- Tributyl-1-propen-1-yl-phosphonium chloride
- Test material form:
- other: solid : wax like
- Details on test material:
- Homogeneity : homogeneous if totally melted
Note: For every use the test item was completely melted and the melted test item
was used for the test.
Constituent 1
impurity 1
impurity 2
- Specific details on test material used for the study:
- Designation in Test Facility: 18032901G
Date of Receipt: 29. Mar. 2018
Condition at Receipt Room temperature, in proper conditions
Batch no. INT 18 192
Appearance Beige crystallised mass
Homogeneity homogeneous if totally melted
Vapour pressure <0.1 hPa at 30 °C
Stability in solvents H2O: unknown; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Solubility in solvents H2O: > 1 g/L; EtOH: unknown; acetone: unknown; CH3CN: unknown; DMSO: unknown
Production date not stated
Expiry date 17. Oct. 2019
Storage Room Temperature (20 ± 5°C)
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- All solutions were sterilized before use.
Composition of the Solutions:
Stock Solution I
NH4Cl 1500 mg
MgCl2*6H2O 1200 mg
CaCl2*2H2O 1800 mg
MgSO4*7H2O 1500 mg
KH2PO4 160 mg
H2O deionised ad 1000 mL
Stock Solution II
FeCl3*6H2O 64 mg
Na2EDTA*2H2O 100 mg
H2O deionised ad 1000 mL
Stock Solution III
H3BO3 185 mg
MnCl2*4H2O 415 mg
ZnCl2 3 mg
CoCl2*6H2O 1.5 mg
CuCl2*2H2O 0.01 mg
Na2MoO4*2H2O 7 mg
H2O deionised ad 1000 mL
6.4.4 Stock Solution IV
NaHCO3 50 g
H2O deionised ad 1000 mL
6.4.5 Algal medium
Stock solution I 10.0 mL
Stock solution II 1.0 mL
Stock solution III 1.0 mL
Stock solution IV 1.0 mL
H2O deionised ad 1000 mL
Stock solutions I and III and the deionised water were sterilized using an autoclave. Stock solutions II and IV were sterilized using filtration. The algal medium corresponds to the OECD TG 201 medium.
Deviations from the nominal weighted loads were less than 5 %.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Unicellular freshwater green alga.
Genus Desmodesmus
Species subspicatus
SAG Strain Number 86.81
Taxonomic position Chlorophyta - Chlorophyceae
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 19.8 – 22.2 °C
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a separate reference test.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- (Growth Rate)
- Effect conc.:
- ca. 1 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Remarks:
- (Yield)
- Effect conc.:
- ca. 1 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Remarks:
- (Growth Rate)
- Effect conc.:
- ca. 3.2 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Remarks:
- (Yield)
- Effect conc.:
- ca. 3.2 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Remarks:
- Growth Rate
- Effect conc.:
- ca. 3.41 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- Growth Rate
- Effect conc.:
- ca. 24.38 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Remarks:
- Yield
- Effect conc.:
- ca. 0.74 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- Yield
- Effect conc.:
- ca. 4.02 mg/L
- Details on results:
- Note: According to the guideline, NOEC is determined by comparing of the respective treatment with the blank control. Statistically insignificant variation is considered as “no observed effect”, although the EC10 which is read from the graph toxicity vs. concentration may lie lower
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- One valid experiment was performed.
The study was performed using 6 concentrations ranging from 0.1 to 32 mg/L (nominal concentration). Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were de-termined from the cell number at the respective observation times.
According to CLP classification criteria, the substance is not classified as acute (short-term) aquatic toxicity
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 32 mg/L
At the start and at the end of the test, the content of the test item in the test solutions was determined using LC-MS.
The measured concentrations lay between 110 % and 117 % of the nominal concentrations at the beginning of the test and between 99 % and 110 % of the nominal concentrations at the end of the test. Therefore, the determination of the results was based on the nominal concentrations (cf. Guidance Doc No. 23 §176).
The 72h-EC50 values of potassium dichromate were determined in a separate reference test. For the estimation of the 72h-EC50 values of the positive control, the fits showed suffi-cient statistical correspondence of the data with the dose-response-equation. The values were within the range of the laboratory.
The pH of the blank control should not fluctuate by more than 1.5 units. The change was 0.9 units in the blank control.
All validity criteria were met.
No observations were made which might cause doubts concerning the validity of the study outcome. The result of the test can be considered valid.
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