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EC number: 250-280-5 | CAS number: 30673-36-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 - 07 Sep 2018
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- 2011
- Deviations:
- yes
- Remarks:
- The light intensity was 520 lx below and 420 lx above the recommended range. The range was also outside the ± 15% recommended range stated in OECD 201. A minimum temperature of 20.4°C was recorded, 0.6°C below the OECD 201 recommended range.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Date of certificate: 11.03.2017 (The Department of Health of the Government of the United Kingdom)
Test material
- Reference substance name:
- Butyl decanoate
- EC Number:
- 250-280-5
- EC Name:
- Butyl decanoate
- Cas Number:
- 30673-36-0
- Molecular formula:
- C14H28O2
- IUPAC Name:
- butyl decanoate
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentrations, taken at start and end of the 72 hour exposure period.
- Sampling method: Samples were taken from remaining test media after filling test vessels for 0 hours and pooled replicate flasks for 72 hours.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Stock solutions were prepared separately by adding the appropriate amount of test substance to solvent (DCM). The total volume of the solvent in the stock solution was 10 mL. The test concentrations were prepared by adding 50 µL of the corresponding stock solution to a total volume of 500 mL of algae medium. Measured amounts of stock solution was added to the correct amount of dilution water.
- Chemical name of vehicle: Methanol for the range finding test and Dichloromethane (DCM) for the definitive test.
- Volume of solvent in solvent control: 50 µL
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: CCAP 278/4
- Source: Culture Collection of Algae and Protozoa, SAMS Ltd, Scottish Marine Institute, OBAN, Argyll PA37 1QA, Scotland, United Kingdom.
- Method of cultivation: In Deionised water with added nutrients according to OECD 201. Temperature: 21.0 – 21.3°C, Illumination: 5480 - 7910 lx continuous white light, Orbiting: set to 200 rpm
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 20.4 – 21.7°C
- pH:
- 7.81 – 8.40
- Nominal and measured concentrations:
- Nominal test substance concentrations: solvent control (DCM), 0 (control), 0.625, 1.250, 2.5, 5, 10 mg/L
Mean measured concentrations: 0 (control), 0.134, 0.376, 0.410, 1.201, 1.579 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flask, glass (nominal 250 mL)
- Fill volume: 100 mL
- Initial cells density: 10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Deionised water with added nutrients according to OECD 201
OTHER TEST CONDITIONS
- Sterile test conditions:no
- Adjustment of pH:no
- Photoperiod: continuous white light
- Light intensity and quality: 5480 - 8420 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Cell densities were measured microscopically in sextuplicate at 24 hours for the control and triplicate thereafter, and in triplicate at 24, 48 and 72 hours (±2h) for the test concentrations by direct cell counts using a haemocytometer and microscope.
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: solvent control (methanol), 0, 0.1, 1.0, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: EC50 (72 h): 1 - 10 mg/L (nominal) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.731 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.679 - 0.793 (95% confidence limits)
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.394 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 0.388 - 0.401 (95% confidence limits)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.376 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in control and solvent control: yes
- Observation of abnormalities: no - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 0.657 mg/L (date 23 to 26 July 2018) - Reported statistics and error estimates:
- The EC50 values were calculated against the solvent control and where possible, estimated graphically with 95% confidence limits calculated according to the method of ToxCalc™ Version 5.0 “Comprehensive Toxicity Data Analysis and Database Software”, copyright 1994-1996.
Any other information on results incl. tables
Table 1: Percent inhibition
Mean measured concentration (mg/L) |
Percent reduction in growth rate |
Percent reduction in yield |
||
0 - 48 hours |
0 - 72 hours |
0 - 48 hours |
0 - 72 hours |
|
0.134 |
-39 |
-12 |
-125 |
-58 |
0.376 |
-40 |
-11 |
-122 |
-53 |
0.410 |
19 |
13 |
41 |
40 |
1.201 |
81 |
95 |
91 |
99 |
1.579 |
97 |
100 |
98 |
100 |
Note: Negative numbers indicate an increase in growth compared to the solvent controls.
Table 2: Mean initial cell density: Approximately 1 x 104cells/ml based upon inoculation volume, not counted microscopically.
Mean measured concentration (mg/L) |
Replicate |
Cell density measurements (cells/ml x 104) |
||
24 hours |
48 hours |
72 hours |
||
0 (Control) |
1 |
5.5 |
28.0 |
93.7 |
2 |
4.3 |
20.7 |
74.3 |
|
3 |
3.3 |
17.0 |
64.7 |
|
4 |
2.3 |
18.3 |
66.0 |
|
5 |
3.8 |
19.7 |
76.0 |
|
6 |
4.7 |
24.7 |
88.7 |
|
Mean |
4.0 |
21.4 |
77.2 |
|
Solvent control |
1 |
2.3 |
13.0 |
64.7 |
2 |
2.0 |
13.7 |
55.7 |
|
3 |
2.3 |
8.3 |
38.0 |
|
4 |
2.5 |
10.3 |
41.7 |
|
5 |
1.7 |
4.7 |
45.0 |
|
6 |
1.5 |
3.7 |
38.0 |
|
Mean |
2.1 |
9.0 |
47.2 |
|
0.134 |
1 |
3.7 |
14.7 |
58.7 |
2 |
1.0 |
15.3 |
65.3 |
|
3 |
3.3 |
26.7 |
98.0 |
|
Mean |
2.7 |
18.9 |
74.0 |
|
0.376 |
1 |
3.7 |
22.0 |
83.7 |
2 |
3.0 |
15.3 |
61.0 |
|
3 |
3.3 |
18.7 |
70.3 |
|
Mean |
3.3 |
18.7 |
71.7 |
|
0.410 |
1 |
1.0 |
4.7 |
23.7 |
2 |
1.0 |
8.0 |
33.7 |
|
3 |
1.3 |
4.3 |
29.0 |
|
Mean |
1.1 |
5.7 |
28.8 |
|
1.201 |
1 |
0.3 |
0.7 |
1.0 |
2 |
1.7 |
2.7 |
1.0 |
|
3 |
0.3 |
1.7 |
1.7 |
|
Mean |
0.8 |
1.7 |
1.2 |
|
1.579 |
1 |
0.7 |
1.0 |
1.3 |
2 |
1.3 |
1.7 |
0.3 |
|
3 |
1.0 |
0.7 |
0.7 |
|
Mean |
1.0 |
1.1 |
0.8 |
Table 3 Effect values
Exposure Period (hours) |
ErCx value mg/L (95% confidence limits) |
EyCxvalue mg/L (95% confidence limits) |
||||
ErC10 |
ErC20 |
ErC50 |
EyC10 |
EyC20 |
EyC50 |
|
0 to 48 |
0.386 (0.382 - 0.392 |
0.395 (0.389 - 0.408) |
0.663 (0.397 - 0.957) |
0.381 (0.380 - 0.383) |
0.386 (0.384 - 0.390) |
0.401 (0.396 - 0.411) |
0 to 72 |
0.394 (0.388 - 0.401) |
0.422 (0.395 - 0.478) |
0.731 (0.679 - 0.793) |
0.382 (0.380 - 0.384) |
0.388 (0.386 - 0.391) |
0.406 (0.400 - 0.452) |
NOEC (0-72h) |
0.376 mg/L (Determined byBonferroni t Test)$ |
0.410 mg/L (Determined by Bonferroni t Test)$ |
Table 4: Validity criteria for OECD 201.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The biomass in the control and solvent control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. |
77.2 |
yes |
The mean coefficient of variation for section-by-section specific growth rates (days 0-1, 1-2 and 2-3, for 72-hour tests) in the control and solvent control cultures must not exceed 35% |
19.08% (control) 47.56% (solvent control)* |
see below |
The coefficient of variation of average specific growth rates during the whole test period in replicate control and solvent control cultures must not exceed 7% in tests with Pseudokirchneriella subcapitata and Desmodesmus subspicatus. For other less frequently tested species, the value should not exceed 10%. |
5.76% |
yes |
*These do not fulfil all the validity criteria of the study and the solvent control is not compliant with the OECD Guideline for Testing of Chemicals reference 201 Alga, Growth Inhibition Test 2011. Nevertheless, as the control replicates passed all validity criteria, the solvent control cell density and rate of growth was sufficient and EC50’sand NOEC/LOEC values were achieved, all effects been attributed the test material and statistical analysis has been conducted in comparison to the solvent control. The cell density in the control cultures increased by the required amount (a factor of at least 16) within the 72-hour test period. Also, there were no cell morphological abnormalities within the control units. Therefore, any changes in cell density in the test concentrations can be attributed to the test substance. In the opinion of the study director, this had no detrimental effect on the validity and integrity of this study.
Applicant's summary and conclusion
- Conclusions:
- For validity criteria please see Table 4 at "Any other information on results incl. tables"
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