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EC number: 452-330-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Remarks:
- The long-term fish toxicity study was conducted solely to comply with a non-EU national registration requirement, and has been provided here in accordance with REACH, Article 22(1)e.
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP
- Qualifier:
- according to guideline
- Guideline:
- other: HJ/T 153-2004, The guidelines for the testing of chemicals [S] Beijing SEPA 2004
- Qualifier:
- according to guideline
- Guideline:
- other: CRC-MEP. The Guidelines for the Testing of Chemicals, Effects on Biotoc Systems [M] 2nd Edition Beijing: China Environment Press 2013 113-120
- Version / remarks:
- 2nd edition
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: GB/T 21807-2008, Chemicals-Fish acute toxicity test, Beijing SAC 2008
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.15 (Fish, Short-term Toxicity Test on Embryo and Sac-fry Stages)
- GLP compliance:
- no
- Remarks:
- The study was conducted under a National quality assurance scheme (HJ/T 155-2004) similar to OECD GLP
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: All test concentrations (1, 2, 4, 8 and 10 mg/L)
- Sampling method: 10 mL water samples were taken from the middle of each aquarium, in duplicate, at the start of the test, and days 3, 9 and 11 days in fresh and aged solutions
- Sample storage conditions before analysis: Not reported - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A 1000 mg/L stock solution was prepared by adding 1.00 g test item in 1 L. The test solution was diluted by the stock solution.
- Controls: Dilution water control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): None reported - Test organisms (species):
- other: Gobiocypris rarus
- Details on test organisms:
- TEST ORGANISM
- Common name: Rare minnow
- Age at study initiation (mean and range, SD): Fertilised eggs were taken at random and examined under microscope to confirm the batch of eggs were in the same cell stage at the start of the test.
- Method of breeding: Male and female fish were paired at 1:1 ration in an incubator. When the male was observed chasing the female, eggs and sperm were collected from the fish and the eggs artificially inseminated to get fertilised eggs.
- Feeding during test: None
ACCLIMATION
- Acclimation period: None - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 11 d
- Remarks on exposure duration:
- 72 hour media reneweal
- Post exposure observation period:
- None
- Hardness:
- 184.9 mg/L as CaCO3
- Test temperature:
- 25.0 °C
- pH:
- 7.63 - 7.96
- Dissolved oxygen:
- 70 - 97 %
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 1.00, 2.00, 4.00, 8.00 and 10.0 mg/L
Mean measured: 0.87, 1.94, 3.89, 7.90 and 10.1 mg/L - Details on test conditions:
- TEST SYSTEM
- Emybro cups (if used, type/material, size, fill volume): Not reported
- Test vessel: 1 L test chambers with sealable inert lids
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: Glass tanks containing 1L test media
- Aeration: Not reported
- Renewal rate of test solution (frequency/flow rate): 72 hour renewal
- No. of fertilized eggs/embryos per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 10 eggs/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, dechlorinated for at least 24 hours
- Total organic carbon: 1.62 mg/L
- Particulate matter: Not reported
- Metals: As = < 0.001 mg/L, Co = 0.0001 mg/L, Pb = < 0.001 mg/L, Co = < 0.001 mg/L, Ni = < 0.001 mg/L, Hg = < 0.0002 mg/L, Cu = < 0.01 mg/L, Cr(VI) = < 0.004 mg/L, Fe = < 0.01, Zn = 0.055 mg/L and Ag < 0.001 mg/L
- Pesticides: Malthion = < 0.025 mg/L, methyl parathion = < 0.002 mg/L, parathion = < 0.003 mg/L
- Chlorine: Not reported
- Alkalinity: Not reported
- Ca/mg ratio: Not reported
- Salinity: Not applicable
- Culture medium different from test medium: No
- Intervals of water quality measurement: Twice per year
OTHER TEST CONDITIONS
- Adjustment of pH: None reported
- Photoperiod: 16-hour light and 8-hour dark cycle
- Light intensity: Not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Stage of embryonic development at test start; hatching and survival of eggs, embryos, larvae and juvenile fish, abnormal appearance and abnormal behaviour at 0, 1, 2, 3, 4, 5, 6, 7 8, 9, 10 and 11 days; and length at the end of the test. - Reference substance (positive control):
- no
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- number hatched
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Survival
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: Survival
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching, survival and malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching, survival and malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching, survival and malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching, survival and malformations
- Key result
- Duration:
- 11 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- length
- Key result
- Duration:
- 11 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- length
- Key result
- Duration:
- 11 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- length
- Key result
- Duration:
- 11 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 10.1 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- length
- Details on results:
- - Mortality/survival at embryo and larval stages:
- Overall mortality/survival: Survival rates ranged between 96.6 % and 100 %
- Days to hatch and numbers hatched: Hatching rates ranged between 93.3 % and 100 %
- Data for length and weight of surviving fish: There was no significant difference between test solution treatment groups and the control group for fish length (Bartlett's test, p > 0.05)
- Type of and number with morphological abnormalities: No abnormal morphology observed in any treatment groups or the control
- Type of and number with behavioural abnormalities: No abnormal behaviour observed in any treatment groups or the control
- Other biological observations: None reported
- Effect concentrations exceeding solubility of substance in test medium: No
- Incidents in the course of the test which might have influenced the results: None - Results with reference substance (positive control):
- No positive control
- Reported statistics and error estimates:
- The hatching, survival and abnormal rates were adjusted by the rates in the control group by Abbott's formula.
Trimmed Spearman-Karber Method was used to calculate the EC50 and confidence limits.
The NOEC and LOEC for fish length growth was compared to the control using a multiple comparison method of Bartlett's test (p > 0.05) - Validity criteria fulfilled:
- yes
- Remarks:
- Dissolved oxygen concentration >60% air saturation value, temperature not differing by more than 1.5°C between test chambers, survival of fertilised eggs in control >80% and post-hatch success in controls >90%.
- Conclusions:
- Based on hatching, survival and malformation the EC50 was >10.1 mg/L, the overall NOEC was 10.1 mg/L and the overall LOEC was >10.1 mg/L, based on mean measured concentrations.
- Executive summary:
The toxicity of the test item to the rare minnow (Gobiocypris rarus) was determined in an 11-day semi-static GLP study according to OECD TG 212. Fertilised eggs were taken at random and examined under microscope to confirm the batch of eggs were in the same cell stage at the start of the test. The fertilised eggs were exposed to nominal concentrations of 1.00, 2.00, 4.00, 8.00 and 10.0 mg/L (mean measured: 0.87, 1.94, 3.89, 7.9 and 10.1 mg/L), and a blank water control. The test medium was exchanged after 72 hours.
Based on hatching, survival and malformation the EC50 was >10.1 mg/L, the overall NOEC was 10.1 mg/L and the overall LOEC was >10.1 mg/L, based on mean measured concentrations.
Reference
Hatching and Survival Observed in the Test Fish:
Time (d) | Nominal concentration (mg/L) | |||||||||||
Blank control | 1.00 | 2.00 | 4.00 | 8.00 | 10.0 | |||||||
Hatching | Survival1 | Hatching | Survival | Hatching | Survival | Hatching | Survival | Hatching | Survival | Hatching | Survival | |
0 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 | 0 | 30 |
1 | 0 | 30 | 0 | 29 | 0 | 30 | 0 | 30 | 0 | 29 | 0 | 30 |
2 | 0 | 30 | 0 | 29 | 0 | 29 | 0 | 30 | 0 | 28 | 0 | 30 |
31 | 10 | 29 | 9 | 29 | 13 | 29 | 11 | 30 | 10 | 28 | 14 | 30 |
4 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
5 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
6 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
7 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
8 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
9 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 30 | 28 | 28 | 29 | 29 |
10 | 29 | 29 | 28 | 28 | 29 | 29 | 30 | 29 | 28 | 28 | 29 | 28 |
112 | 29 | 28 | 28 | 28 | 29 | 28 | 30 | 29 | 28 | 27 | 29 | 28 |
Hatching rates (%) | 96.7 | 93.3 | 96.7 | 100 | 93.3 | 96.7 | 96.7 | 93.3 | 96.7 | 100 | 93.3 | 96.7 |
Survival rates (%)3 | 100 | 100 | 100 | 96.7 | 100 | 96.6 | 100 | 100 | 100 | 96.7 | 100 | 96.6 |
1: Initiation of hatching on the 3rd day
2: The larvae of the control group begin to die of hunger on the 11th day, and the test ended. The hatching rates and survival rates were calculated based on the results of the first 10 days.
3: Survival rates = (survival number/hatching number)*100 %
Abnormal behaviour and abnormal appearence of test fish:
Time (d) | Nominal concentration (mg/L) | |||||
Blank control | 1.00 | 2.00 | 4.00 | 8.00 | 10.0 | |
0 | 30NB | 30NB | 30NB | 30NB | 30NB | 30NB |
1 | 30NB | 29NB & 1 Dead | 30NB | 30NB | 29NB & 1 Dead | 30NB |
2 | 30NB | 29NB & 1 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 30NB |
31 | 29NB & 1 Dead | 29NB & 1 Dead | 30NB | 30NB | 28NB & 2 Dead | 30NB |
4 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 29NB & 1 Dead |
5 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 29NB & 1 Dead |
6 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 29NB & 1 Dead |
7 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 29NB & 1 Dead |
8 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 30NB | 28NB & 2 Dead | 29NB & 1 Dead |
9 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead |
10 | 29NB & 1 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 29NB & 1 Dead | 28NB & 2 Dead | 28NB & 2 Dead |
112 | 28NB & 2 Dead | 28NB & 2 Dead | 28NB & 2 Dead | 29NB & 1 Dead | 27NB & 3 Dead | 28NB & 2 Dead |
NB: normal appearance and behaviour
1: Initiation of hatching on the 3rd day
2: The larvae of the control group begin to die of hunger on the 11th day, and the test ended.
Hatching rate, Survival rate, and normal rate of the Embryos and Larvae at the End of the Test (11 days)
Nominal Concentration (mg/L) | Measured concentration (mg/L) | Adjusted hatching rate, survival rate and normal rate (%)* | ||
Hatching rate | Survival rate | Normal rate | ||
Blank Control | ND | 100 | 100 | 100 |
1.00 | 0.87 | 96.5 | 100 | 100 |
2.00 | 1.94 | 100 | 100 | 100 |
4.00 | 3.89 | 103.4 | 96.7 | 100 |
8.00 | 7.9 | 96.5 | 100 | 100 |
10.0 | 10.1 | 100 | 96.6 | 100 |
EC50 (mg/L, based on measured concentration) | > 10.1 | > 10.1 | > 10.1 | |
95 % CI (mg/L, based on measured concentration) | - | - | - | |
NOEC (mg/L) | 10.1 | |||
LOEC (mg/L) | > 10.1 |
*Adjusted by Abbot's formula, Adjusted hatching rate, survival rate and normal rate were calculated based on the results of day 10
Description of key information
11-day NOEC = 10.1 mg/L, rare minnow (Gobiocypris rarus), OECD TG 212, Guixiang 2016
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10.1 mg/L
Additional information
The toxicity of the test item to the rare minnow (Gobiocypris rarus) was determined in an 11-day semi-static GLP study according to OECD TG 212. Fertilised eggs were taken at random and examined under microscope to confirm the batch of eggs were in the same cell stage at the start of the test. The fertilised eggs were exposed to nominal concentrations of 1.00, 2.00, 4.00, 8.00 and 10.0 mg/L (mean measured: 0.87, 1.94, 3.89, 7.9 and 10.1 mg/L), and a blank water control. The test medium was exchanged after 72 hours.
Based on hatching, survival and malformation the EC50 was >10.1 mg/L, the overall NOEC was 10.1 mg/L and the overall LOEC was >10.1 mg/L, based on mean measured concentrations.
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