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EC number: 907-961-3 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30th March 2018 to 15th October 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- 40 mL of test sample from each replicate were withdrawn and mixed for each group. Collected samples were centrifuged at 2000 rpm for 10 minutes to remove algal cells. The representative samples were divided into two equal portions. One portion was sent for test concentration analysis at 0 h and the second portion was stored at -20 ± 5 ºC.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- A quantity of 250 mg of test item was weighed, and made up to 2500 mL with algal medium in glass bottles (stock A) and retained for continuous stirring using a magnetic stirrer (at 630 rpm) for approximately 24 h at room temperature. After stirring, test solutions were kept for re-equilibrium for approximately 24 h. After phase separation, test solutions were collected from the lower portion using an “L” shaped glass tube without disturbing the phases. A volume of 1500 mL was collected from the lower to middle portion by pipette and used for treatment. Prior to adding test solution to vessels, test vessels were pre-conditioned with the respective test concentration to saturate the surface of the respective vessel to prevent loss of test concentration due to absorption into the vessel walls. Volumes of 199.5 mL from the stock A and 0.5 mL algal culture were taken and mixed into sterile conical flasks of 250 mL capacity to obtain the loading rate of 100.0 mg test item/L. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: ATCC 22662
- Source (laboratory, culture collection): American Type Culture Collection, 10801, University of Boulevard, Manassas, Virginia, 20110-2209, USA; Subculture Maintained at : Section - Ecotoxicology, Jai Research Foundation, Valvada, Dist. Valsad, Gujarat
- Age of inoculum (at test initiation): The pre-culture was prepared two days prior to the commencement of the study
ACCLIMATION
- Culturing media and conditions (same as test or not): same - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Hardness:
- no data
- Test temperature:
- 21 to 23 °C
- pH:
- 7.75 - 8.09
- Dissolved oxygen:
- no data
- Salinity:
- N/A
- Nominal and measured concentrations:
- nominal: 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL conical flasks, fill volumen 200 mL
- Aeration: by agitating continuously at 100 ± 2 rpm, using an orbital shaker during the test period
- Initial cells density: 5244 (mean) cells/mL
- Control end cells density: 781250 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Light intensity and quality: 6673 – 6680 lux (± 15% of the mean value), universal UV- free white fluorescent lamp
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: counting chamber
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.0 (control), 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L
- Results used to determine the conditions for the definitive study:
The percent inhibition of biomass was 0.37, 0.37, 1.06, 1.33 and 2.02 at loading rates of 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L, respectively.
The percent inhibition of growth rate was 0.00, 0.00, 0.00, 0.00 and 0.15 at loading rates of 0.1, 1.0, 10.0, 50.0 and 100.0 mg/L, respectively.
Based on the results of the preliminary range finding study, the main study was restricted to a limit study. The limit study was conducted with a control and at the loading rate of 100.0 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Remarks:
- WAF
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Remarks:
- WAF
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control: yes
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- 72 h ErC50 = 1.15 mg/L, 72 h EyC50 = 0.52 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- Reaction mass of dodecyl methacrylate and tridecyl methacrylate does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the loading rate of 100.0 mg/L. The EL50, NOELR and LOELR value were greater than 100.0 mg/L.
- Executive summary:
This study was performed to assess the effects on the growth of the alga Pseudokirchneriella subcapitata caused by exposure to Reaction mass of dodecyl methacrylate and tridecyl methacrylate under static conditions according to OECD 201.
In the main (limit) test, exponentially growing cultures of P. subcapitata were exposed using water accommodated fraction at loading rates of 0.0 (Control), and 100.0 mg/L. Algal cultures were assessed for growth by visual cell counts at 24, 48, and 72 h.
The stability of the test item in test media was performed during method validation (JRF Study N° 228-2-13-19223), the test item in test media was stable up to 72 h (>80% of nominal concentration). Test media was analysed for active ingredient concentration and stability to assess the concentration and stability of test solution at 0 and 72 h during the main study. The active ingredient concentration of the test item in test media was within an acceptable limit (>80% of nominal concentration).
In the test item exposed group, no significant inhibitory effect was observed in algal growth (biomass), yield and growth rate compared to control group. The test item does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the loading rate of 100.0 mg/L. The EL50, NOELR and LOELR value were greater than 100.0 mg/L.
Reference
Test Culture Conditions
The pH and temperature ranged within 7.75 – 8.09 and 21 - 23°C respectively. The mean illumination values ranged from 6673 – 6680 lux. All parameters were within accepted guideline limits.
Algal Cell Count
In the control, the cell concentration increased by a factor of 149.0 times over the 72 h exposure period [as per OECD 201 (2006) at least 16 times in control]. The coefficient of variance of average specific growth rate during the whole test period (0-3) in replicate control cultures was 0.60%. The mean coefficient of variation for day 0-1, 1-2 and 2-3 in control cultures was 9.3. Thus the coefficient of variation was within accepted guideline limits.
Analysis of Test Concentrations
The stability of C12-13 alkyl methacrylate in test media was performed during method validation (JRF Study N° 228-2-13-19223), C12-13 alkyl methacrylate in test media was stable up to 72 h
(>80% of nominal concentration).
Test media was analysed for C12-13 alkyl methacrylate active ingredient concentration and stability to assess the concentration and stability at 0 and 72 h during the main study. The active
ingredient concentration of C12-13 alkyl methacrylate in test media was within an acceptable limit (>80% of nominal concentration).
Growth Inhibition, EL50 Values, NOELR and LOELR
There was no significant alteration in algal growth (biomass), yield and growth rate of the C12-13 alkyl methacrylate exposed group when compared to the control group. C12-13 alkyl methacrylate
does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the loading rate of 100.0 mg/L. The EL50, NOELR and LOELR value was greater than 100.0 mg/L.
Validity Criteria of the Study
The coefficient of variation of average specific growth rates during the test period in the replicate control cultures was 0.60%. Thus, the validity criterion was met.
The cell concentration in control cultures increased exponentially by a factor of 149.0 within the test period. Thus, the validity criterion was met.
The mean coefficients of variation for day 0-1, 1-2, and 2-3 in control culture was 9.3%. Thus, the validity criterion was met.
Mean Values of Algal Biomass, Yield, Specific Growth Rate
Loading Rate (mg/L) |
Mean Number of Cells Count/mL at |
Specific Growth Rate |
||||
0 h |
24 h |
48 h |
72 h |
Yield |
||
0.0 (Control) |
5244 |
28750 |
152500 |
781250 |
776006 |
1.67 |
100 |
5244 |
28333 |
151250 |
777917 |
772673 |
1.67 |
Description of key information
72 h EL50, NOELR and LOELR value were greater than 100.0 mg/L (Pseudokirchneriella subcapitata, OECD TG 201)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
This study was performed to assess the effects on the growth of the alga Pseudokirchneriella subcapitata caused by exposure to Reaction mass of dodecyl methacrylate and tridecyl methacrylate under static conditions according to OECD 201.
In the main (limit) test, exponentially growing cultures of P. subcapitata were exposed using water accommodated fraction at loading rates of 0.0 (Control), and 100.0 mg/L. Algal cultures were assessed for growth by visual cell counts at 24, 48, and 72 h.
The stability of the test item in test media was performed during method validation (JRF Study N° 228-2-13-19223), the test item in test media was stable up to 72 h (>80% of nominal concentration). Test media was analysed for active ingredient concentration and stability to assess the concentration and stability of test solution at 0 and 72 h during the main study. The active ingredient concentration of the test item in test media was within an acceptable limit (>80% of nominal concentration).
In the test item exposed group, no significant inhibitory effect was observed in algal growth (biomass), yield and growth rate compared to control group. The test item does not inhibit the growth of alga (Pseudokirchneriella subcapitata) up to the loading rate of 100.0 mg/L. The EL50, NOELR and LOELR value were greater than 100.0 mg/L.
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