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Diss Factsheets
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EC number: 202-461-5 | CAS number: 95-87-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data from reliablesource. Though no test report is available at present the data from this source are known to be well documented and the test are performed according to GLP and OECD guidelines.
Data source
Reference
- Reference Type:
- publication
- Title:
- Information on Environmental Toxicity
- Author:
- NITE (National Institute of Technology and Evaluation) Japan
- Year:
- 1 998
- Bibliographic source:
- NITE (National Institute of Technology and Evaluation) Japan, available at: http://www.safe.nite.go.jp/english/db.html
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- not specified
- Remarks:
- probably: yes
Test material
- Reference substance name:
- 2,5-xylenol
- EC Number:
- 202-461-5
- EC Name:
- 2,5-xylenol
- Cas Number:
- 95-87-4
- Molecular formula:
- C8H10O
- IUPAC Name:
- 2,5-dimethylphenol
- Reference substance name:
- Reference substance 001
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test organism
The recommended species is Daphnia magna, but other Daphnia species such as Daphnia pulexs may also be used.
Use organisms that are 24 hr old or younger at the beginning of the exposure. To reduce variation, do not use the first offsprings of the parents. The test organisms must be obtained from healthy parents (i.e., showing no sign of stress under the culture conditions such as a high mortality, the appearance of males or oostegites, a prolonged period before the first offsprings and discoloration) of the same strain.
The parent organisms must be cultured under conditions (light, temperature and water) as same as those employed in the test. When performing the test using water different from that usually used for culturing Daphnia spp., establish an acclimatization period before beginning the exposure. The acclimatization can be performed by culturing the organisms in the material water at the test temperature for at least 48 hr before beginning the exposure. Use the offsprings obtained from acclimatized parents for the test.
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Details on test conditions:
- The following test vessel and equipment are used for the study.
Test vessel
The test vessel or other instrument that contacts the test solution must be made of glass or other chemically inert materials. Cap the vessel loosely to prevent evaporation and dust contamination.
If the test substance is volatile, perform the test in a sealed system. Use a sufficiently large vessel to prevent shortage of dissolved oxygen.
Instrument
Use a dissolved oxygen meter (a microelectrode or other instrument suitable for measuring the dissolved oxygen concentration with a small amount of sample), a pH meter, an appropriate instrument for controlling temperature, etc., for the test.
Material water
Use water suitable for culturing and testing Daphnia spp. It can be natural water (surface water or groundwater), dechlorinated tap water or artificially prepared water (e.g., Appendix Table 1), but must satisfy the conditions listed in Appendix Table 2.
Do not use Elendt M4 or M7 media or water containing chelating agents for testing metal-containing substances. The water hardness should be 250 mg/L or smaller in terms of calcium carbonate concentration, and the pH should be 6-9.
Aerate the material water before using it for the test.
Test solution
To prepare a test solution of each concentration, directly dissolve the required amount of the test substance in the material water, or prepare a stock solution of the test substance at an appropriate concentration and dilute it with the material water. Follow the descriptions in "Preparation of the test solution" under "III. General rules".
Perform the test without adjusting the pH. If the pH of the material water is not within the range of 6-9, it is recommended to perform an additional test after adjusting the pH to that observed prior to the addition of the test substance. Perform the pH adjustment through a method causing no change in the concentration, chemical reaction or precipitation of the test substance. Preferably, use HCl or NaOH for the pH adjustment.
Test method
The test can be performed under a static, semi-static or flow-through condition. If the test substance concentration is unstable, a semi-static or flow-through test is recommended.
Exposure period
Perform the exposure period for 48 hr.
Volume and number of test organisms
Volume: Use at least 2 mL or the test solution per organism.
Number of test organisms: Use at least 20 organisms for each of the test concentrations and the control. Preferably, divide the organisms into 4 groups of 5 organisms.
Test concentrations
Adopt a concentration range comprising at least 5 concentrations that are setup in a geometric progression, preferably at a geometric ratio of within 2.2. The highest test concentration preferably induces 100% immobilization, but concentrations of 100 mg/L or higher do not need to be tested. Preferably, no effect is observed at the lowest concentration.
Perform a control, and additionally an auxiliary control if using any auxiliary.
Culture method
Illumination: The photoperiod is preferably set to 16 hr light and 8 hr dark. The test can be conducted in dark if the test substance is unstable against light.
Temperature: The temperature is set within the range of 18°C - 22°C, with variations among the test vessels of ± 1.0°C.
Dissolved oxygen concentration: It must be kept at 3 mg/L or higher. In principle, do not perform aeration during the exposure period.
Feeding: Do not feed the organisms.
Beginning of the exposure to the test substance
Start the exposure by transferring a specified number of organisms established in 5-3. to each test vessel.
Observation
Observe the mobility of the organisms at least twice, i.e., at 24 and 48 hr after the beginning of the exposure. The organisms are considered as being immobilized when they do not move for 15 sec after the test vessel is gently shaken. During observation, record any anomaly in behavior or appearance besides immobilization. - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 48 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.