Registration Dossier
Registration Dossier
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EC number: 616-586-1 | CAS number: 78068-85-6
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-chloro-1-fluoro-4-(trifluoromethyl)benzene
- EC Number:
- 616-586-1
- Cas Number:
- 78068-85-6
- Molecular formula:
- C7H3ClF4
- IUPAC Name:
- 2-chloro-1-fluoro-4-(trifluoromethyl)benzene
- Reference substance name:
- 1-Chloro-2-fluoro-4-(trifluoromethyl)benzene
- Cas Number:
- 32137-20-5
- Molecular formula:
- C7H3ClF4
- IUPAC Name:
- 1-Chloro-2-fluoro-4-(trifluoromethyl)benzene
- Test material form:
- liquid
Constituent 1
impurity 1
- Specific details on test material used for the study:
- Purity: 89.6 % [a/a]
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- HPLC
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Length: 2.57 cm (mean)
Weight: 0.49 g (mean)
Loading: 0.86 g fish / L test solution
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Remarks:
- Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove chlorine.
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 52-77 mg CaCO3/L
- Test temperature:
- Test: 21.2-22.8 °C
Control: 21.2-22.8 °C - pH:
- Test: 6.97-7.32
Control: 6.92-7.31 - Dissolved oxygen:
- Test: 84-99 %
Control: 82-100 % - Nominal and measured concentrations:
- Measured (mean): 1.42, 2.51, 5.65, 12.3 and 34.0 mg/L
- Details on test conditions:
- Due to high vapour pressure of the test item daily exchange of the test media was carried out.
Glass aquaria; one replicate per concentration; 7 fish per replicate; no aeration with regard to the high vapour pressure of the test item; aquaria were covered by a glass plate; a natural photoperiod occurred during the test.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 2 h
- Dose descriptor:
- LC50
- Effect conc.:
- 23.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Confidence interval: 12.3-34.0 mg/L
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.98 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Confidence interval: 5.65-12.3 mg/L
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.98 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Confidence interval: 5.65-12.3 mg/L
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.08 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Confidence interval: 2.51-5.65 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.08 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Confidence interval: 2.51-5.65 mg/L
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.42 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In this study 3-Chloro-4-fluorobenzotrifluoride was found to be toxic to Danio rerio after 96 h at the mean measured concentration level of 2.51 mg/L and higher.
LC50(96h): 4.08 (2.51-5.65) mg/L
NOEC (96h): 1.42 mg/L
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