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EC number: 277-531-1 | CAS number: 73545-11-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2019
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 7-(4-ethyl-1-methyloctyl)quinolin-8-ol
- EC Number:
- 277-531-1
- EC Name:
- 7-(4-ethyl-1-methyloctyl)quinolin-8-ol
- Cas Number:
- 73545-11-6
- Molecular formula:
- C20H29NO
- IUPAC Name:
- 7-(5-ethylnonan-2-yl)quinolin-8-ol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Sponsor / 1702-18-01/O
- Expiration date of the lot/batch: 25. Mar. 2021
- Purity test date: 26. Mar. 2018
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Stored in a tightly closed vessel at room temperature under dry conditions in the dark
- Solubility and stability of the test substance in the solvent/vehicle:
Stability unknown. Water solubility <0.1g/L; Acetione >1 g/L; ethanol, Ch3CN & DMSO, unknown
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing:
The carbon content of 80.22% was calculated by molecular formula given by the sponsor. The test item was weighed directly, the amounts were calculated from the crbon contnent of the test item
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, 67435 NW-Lachen-Speyerdorf. Date of collection: 28 September 2018, Batch number: 20180928
- Pretreatment: The sludge was filtrated, washed with test medium (2x) and re-suspended in test medium. It was then aerated until use. The dry matter was determined to contain 4940 mg of suspended solids/L.
- Concentration of inoculum: 25.0 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 20 mg/L
- Based on:
- other: organic carbon
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1 litre mineral medium contains; 10mL Solution (A), 1mL Solution (B), 1 mL Solution (C), 1 mL Solution (D)
A) 8.5g KH2PO4
21.75g K2HPO4
33.4g Na2HPO4*2H2O
0.5g NH4Cl
ad 1000mL H2O demin.
pH 7.4
B) 27.5g CaCl2
ad 1000 mL H2O demin.
C) 22.5g MgSO4*7H2O
ad 1000mL H2O demin.
D) 0.25g FeCl3*6H2O
0.4g Na2EDTA*2H2O
ad 1000mL H2O demin.
- Additional substrate: n/a
- Solubilising agent (type and concentration if used): not used
- Test temperature: 20.1 - 22.8 ºC witjut direct light
- pH:
- pH adjusted: no
- Aeration of dilution water: no
- Suspended solids concentration:
- Continuous darkness: yes/no
- Other:
TEST SYSTEM
- Culturing apparatus: 1500mL flasks
- Number of culture flasks/concentration: 2 replicates
- Method used to create aerobic conditions: Test vessels were aerated with purified (by active charcole), CO2-scrubbeed, moistened air. The scrubbing of carbon dioxide was achieved by bubbling the purified air through a flask containing 1.5M NaOH. To contrl the absence of CO2, the air was then led through a flask containing a solution of Ba(OH)2 befre reaching the test vessels.
- Method used to create anaerobic conditions:
- Measuring equipment: TOC multi N/C 2100S, Analytik Jena
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2 and volatile organics if used: Scrubber containing 0.25M NaOH
- Other:
SAMPLING
- Sampling frequency: Day 0, 3, 7, 9, 11, 14, 17, 23 & 29
- Sampling method: From each front scrubber flask, samples were taken i order to determine the emitted Co2. The sample volume was 1mL. The resulting change in the volume of the front flask was considered in the calculation of emitted CO2. On day 28, 5mL HCl 2M was added to each test flask in order to drive off dissolved CO2. On day 29, samples from both scrubber flasks were taken.
- Sterility check if applicable:
- Sample storage before analysis:
- Other:
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation reached 2.4 %. Both replicates of the test item showed good correspondence.
If degradation in the toxicity flask is below 25 % after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 35.8 % after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 24.9 mg/L”.
For pure substances ready biodegradability is defined in the guidelines as degradation surpassing 60 % within 10 days after reaching a level of 10 %.
Because the test item is a mixture, the 10-day window has not to be taken into account. For the test item 7-(4-ethyl-1-methyloctyl)quinolin-8-ol no biodegradation was observed after 28 days. The 10-day-window could not be determined. The criterion of reaching 60% of degradation after 28 days is not met. 7-(4-ethyl-1-methyloctyl)quinolin-8-ol is therefore considered as “not readily biodegradable”.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid.
Any other information on results incl. tables
Validity
Parameter | Criterion | Found | Assessment |
IC content of test item solution in medium | < 5% of TC | 0% | Valid |
CO2emitted by the controls | < 70 mg/L | 11.4 mg/L | Valid |
Difference within replicates | < 20% | 5.9% | Valid |
Degradation of positive control >60% | <= 14 days | 10 days | Valid |
Degradation in the toxicity flask on day 14 | > 25% | 35.8% | Non-toxic |
Tables
In the following tables, the IC values (given in mg/L) which were measured in the samples of the scrubber flasks are stated.
Table8.1 -a IC values in mg/L of apparatus blanks, blank controls, front scrubber
Day | Apparatus blank 1 | Apparatus blank 2 | Blank control 1 | Blank control 2 |
0 | 3.08 | 3.42 | 3.82 | 3.30 |
3 | 5.83 | 7.47 | 10.23 | 27.11 |
7 | 9.08 | 11.06 | 17.18 | 37.5 |
9 | 12.10 | 13.31 | 20.64 | 54.43 |
11 | 12.90 | 15.05 | 24.55 | 62.56 |
14 | 14.27 | 16.80 | 28.49 | 67.74 |
17 | 16.05 | 18.07 | 30.08 | 70.79 |
23 | 21.68 | 22.44 | 33.55 | 90.80 |
29 | 28.82 | 30.34 | 41.86 | 119.10 |
Table 8.1 -b IC values in mg/L of positive control, test flasks, front scrubber
Day | Positive Control 1 | Positive Control 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control | |
0 | 3.00 | 3.81 | 3.80 | 5.57 | 9.56 | 3.45 | |
3 | 19.96 | 14.32 | 12.77 | 17.08 | 14.46 | 24.71 | |
7 | 133.48 | 155.99 | 25.07 | 26.85 | 19.10 | 109.75 | |
9 | 196.35 | 222.97 | 32.84 | 37.08 | 21.39 | 228.34 | |
11 | 262.22 | 263.31 | 37.25 | 43.59 | 23.13 | 243.27 | |
14 | 305.66 | 302.25 | 40.90 | 53.93 | 27.17 | 273.66 | |
17 | 325.59 | 313.56 | 62.78 | 58.78 | 32.03 | 280.48 | |
23 | 357.17 | 335.87 | 54.02 | 66.32 | 34.28 | 313.73 | |
29 | 389.43 | 352.28 | 67.62 | 88.94 | 43.90 | 320.24 |
In the following tables, the IC values which were measured in the samples of the back scrubber flasks are stated.
Table 8.1 -c IC values in mg/L of blank controls, apparatus blanks, back scrubber
Day | Apparatus blank 1 | Apparatus blank 2 | Blank control 1 | Blank control 2 |
0 | 4.06 | 4.01 | 3.48 | 3.90 |
29 | 3.77 | 3.82 | 4.36 | 6.07 |
Table 8.1 -d IC values in mg/L of positive control, test flasks, back srubber
Day | Positive Control 1 | Positive COntrol 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control |
0 | 3.79 | 4.69 | 3.62 | 9.50 | 7.91 | 5.48 |
29 | 4.47 | 4.09 | 4.53 | 5.98 | 8.4 | 13.10 |
Net IC
For each flask, the net IC was calculated by subtracting the mean IC value of the apparatus blanks of the corresponding sampling date from the remaining IC values. Exception: Values of day 0 do not need to be corrected.
The value of day 0 of the apparatus blank was subtracted from the apparatus blanks of the corresponding sampling dates in advance.
The net IC values are presented in the following table.
Table 8.1 -e Net IC-values in mg/L of front scrubber flasks
Day | Blank Cntrol 1 | Blank Control 2 | Positive Control 1 | Positive Control 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control |
0 | 3.8 | 3.3 | 3.0 | 3.8 | 3.8 | 5.6 | 9.6 | 3.5 |
3 | 6.8 | 23.7 | 16.6 | 10.9 | 9.4 | 13.7 | 11.1 | 21.3 |
7 | 10.4 | 30.7 | 126.7 | 149.2 | 18.2 | 20.0 | 12.3 | 102.9 |
9 | 11.2 | 45.0 | 186.9 | 213.5 | 23.4 | 27.6 | 11.9 | 218.9 |
11 | 13.8 | 51.8 | 251.5 | 252.6 | 26.5 | 32.9 | 12.4 | 232.6 |
14 | 16.2 | 55.5 | 293.4 | 290.0 | 28.6 | 41.6 | 14.9 | 261.4 |
17 | 16.3 | 57.0 | 311.8 | 299.8 | 49.0 | 45.0 | 18.2 | 266.7 |
23 | 14.7 | 72.0 | 338.4 | 317.1 | 35.2 | 47.5 | 15.5 | 294.9 |
29 | 15.5 | 92.8 | 363.1 | 325.9 | 41.3 | 62.6 | 17.6 | 293.9 |
Table 8.1 -f Net IC-values in mg/L of back scrubber flasks
Day | Blank Control 1 | Blank Control 2 | Positive Control 1 | Positive Control 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control |
0 | 3.5 | 3.9 | 3.8 | 4.7 | 3.6 | 9.5 | 7.9 | 5.5 |
29 | 4.6 | 6.3 | 4.7 | 4.3 | 4.8 | 6.2 | 8.6 | 13.3 |
In the followingtable, the pH at the end of the test (before addition of HCl) is given:
Table 8.1 -g pH in test flasks on day 28
Day | Blank control 1 | Blank control 2 | Positive control 1 | Positive control 2 | Test 1 | Test 2 | Abiotic control | Toxicity control |
28 | 7.6 | 7.6 | 7.4 | 7.4 | 7.5 | 7.5 | 6.9 | 7.4 |
Equations
Emitted carbon in mg/L
Emitted carbon in mg/L test solution in the respective vessel at time t was calculated using the following equation:
emittC= ((IC(t)-IC(0))*VolNaOH(t)) / Vol_Testvessel
with
emittC emitted carbon in mg/L test solution
IC(t) net inorganic carbon in mg/L NaOH in the respective vessel at time t
IC(0) net inorganic carbon in mg/L NaOH in the respective vessel at the start of the test
VolNaOH (t) remaining volume NaOH in L in the scrubber at time t (Volume at t=0 (here: 0.1L) -∑(all sample volumes up to time t))
Vol_Testvessel test vessel volume in L (here: 1.5)
For day 29, the IC content of both scrubber flasks was taken into account.
Calculation of emitted carbon is necessary for the assessment of validity. The value obtained with the equation is multiplied with 3.667 (44/12) in order to obtain emitted CO2.
Degradation in %
The percentage biodegradation in the test flasks was calculated from:
% degradation = ((emittedC(Test) in mg/L - Mean emittedC(Controls)in mg/L) / addedC in mg/L)*100%
Degradation in positive control and toxicty flasks was calculated analogously.
Abiotic degradation was calculated from:
% degradation = (emittedC(abiotic)in mg/L) / (addedC in mg/L) * 100%
Calculation Results
Emitted Carbon in mg/L
In the following table, the calculated emitted carbon (from net IC given earlier and equation stated above) is presented.
Table 8.3 -a Emitted carbon in mg/L
Day | Blank Control 1 | Blank Control 2 | Positive Control 1 | Positive Control 2 | Test 1 | Test 2 | Abiotic Control | Toxicity Control |
3 | 0.20 | 1.35 | 0.90 | 0.47 | 0.37 | 0.54 | 0.10 | 1.18 |
7 | 0.43 | 1.79 | 8.08 | 9.50 | 0.94 | 0.94 | 0.18 | 6.50 |
9 | 0.48 | 2.69 | 11.89 | 13.56 | 1.27 | 1.43 | 0.15 | 13.93 |
11 | 0.64 | 3.11 | 15.90 | 15.92 | 1.45 | 1.75 | 0.18 | 14.66 |
14 | 0.78 | 3.30 | 18.39 | 18.12 | 1.57 | 2.29 | 0.34 | 16.34 |
17 | 0.78 | 3.36 | 19.35 | 18.55 | 2.83 | 2.47 | 0.54 | 16.50 |
23 | 0.68 | 4.26 | 20.79 | 19.42 | 1.95 | 2.60 | 0.37 | 18.07 |
29 | 0.72 | 5.49 | 22.09 | 19.76 | 2.30 | 3.50 | 0.49 | 17.81 |
Degradation Values
In the following table, the percentage biodegradation is presented:
Table 8.3 -b Degradation values in %
Day | Positive Control 1 | Positive Control 2 | Positive Control Mean | Test 1 | Test 2 | Test Mean | Abiotic Control | Toxicity Control |
3 | 0.6 | -1.5 | -0.5 | -2.0 | -1.2 | -1.6 | 0.5 | 1.0 |
7 | 34.9 | 42.0 | 38.5 | -0.8 | -0.8 | -0.8 | 0.9 | 13.5 |
9 | 51.6 | 60.0 | 55.8 | -1.6 | -0.8 | -1.2 | 0.7 | 30.9 |
11 | 70.3 | 70.3 | 70.3 | -2.1 | -0.6 | -1.3 | 0.9 | 32.0 |
14 | 81.9 | 80.5 | 81.2 | -2.3 | 1.2 | -0.6 | 1.6 | 35.8 |
17 | 86.5 | 82.5 | 84.5 | 3.7 | 2.0 | 2.9 | 2.6 | 36.1 |
23 | 91.8 | 84.9 | 88.3 | -2.6 | 0.7 | -0.9 | 1.8 | 39.0 |
29 | 95.1 | 83.4 | 89.2 | -3.9 | 2.0 | -1.0 | 2.4 | 36.8 |
Because the values of day 29 are the sum of the IC vlues in both scrubber flasks, an increase (IC values in flasks B of the test higher than in those of the control) or a decrease (IC values in flasks B of the test lower than in those of the control) of degradation can be observed.
As the measured IC values in the test flasks are very low, measurement uncertainties lead to negative degradation values while in fact no degradation has taken place.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- All validity criteria were met.
Degradation behaviour of positive control and toxicity control was normal. Abiotic degradation reached 2.4 %. Both replicates of the test item showed good correspondence.
If degradation in the toxicity flask is below 25 % after 14 days, the test item can be considered as toxic towards the inoculum. As degradation in the toxicity flask was 35.8 % after 14 days, the test item can be stated as “not toxic towards the inoculum in a concentration of 24.9 mg/L”.
For pure substances ready biodegradability is defined in the guidelines as degradation surpassing 60 % within 10 days after reaching a level of 10 %.
For the test item 7-(4-ethyl-1-methyloctyl)quinolin-8-ol no biodegradation was observed after 28 days. The 10-day-window could not be determined. The criterion of reaching 60% of degradation after 28 days is not met. 7-(4-ethyl-1-methyloctyl)quinolin-8-ol is therefore considered as “not readily biodegradable”.
No observations were made which might cause doubts concerning the validity of the study outcome.
The result of the test can be considered valid. - Executive summary:
The test item 7-(4-ethyl-1-methyloctyl)quinolin-8-ol was tested using a concentration of nominally 20 mg organic carbon/L7-(4-ethyl-1-methyloctyl)quinolin-8-ol in test medium following OECD 301B and EU-Method C.4-C.
Aniline was chosen as positive control.
Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days.
All validity criteria were met. Degradation of the positive control surpassed the pass level of 60 % after 10 days.
The following data were determined for the test item7-(4-ethyl-1-methyloctyl)quinolin-8-ol:
10-day-window: not detected
degradation at the end of the test 0 %
pass level following guideline: 60 % at the end of 10-day-window fo pure substancesrespective 60 % at the end of the test for mixtures
For the test item7-(4-ethyl-1-methyloctyl)quinolin-8-ol no biodegradation was observed after 28 days. The 10-day-window could not be determined. The criterion of reaching 60% of degradation after 28 days is not met.7-(4-ethyl-1-methyloctyl)quinolin-8-ol is therefore considered as “not readily biodegradable”.
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