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EC number: 218-039-9 | CAS number: 2040-64-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Germany
Test material
- Reference substance name:
- Tetradecyl myristate
- EC Number:
- 221-787-9
- EC Name:
- Tetradecyl myristate
- Cas Number:
- 3234-85-3
- Molecular formula:
- C28H56O2
- IUPAC Name:
- tetradecyl myristate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control, 100 mg/L. The concentrations of tetradecyl myristate in the test media were analysed at test start directly after distribution of the stock test item WAF into the 60 mL glass beakers (fresh test media at test start). Furthermore, a second sample of the stock WAF was taken at this time to investigate a possible 'wall effect' of the used glass beakers. Another sample was taken at the end of the test after 48 hours (aged test media at test end).
- Sampling method: As the volume of the analytical sub-sample required for sampling was large compared to the total test media volume, the test item concentration was determined from samples taken from additionally prepared glass beakers. Five millilitres were pipetted directly into 20 mL extraction (centrifuge) tubes and 15 mL of purified water added. The pipette tips were rinsed multiple times and the surface wiped with a lint free paper towel prior taking the analytical sub-sample.
- Sample storage conditions before analysis: The samples were measured immediately after sampling without further storage.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance pellets were crushed by mortar and pestle and the Water Accommodated Fraction (WAF) prepared with the test item powder. A test preparation with a loading of 100 mg/L dilution water was prepared according to the OECD guideline No. 23, and stirred slowly at 300 rpm to avoid bubble and foam formation using a stirring bar on a magnetic stirrer for 48 h at room temperature (about 20 °C). Finally, the test preparation was filtered using a 0.2 µm membrane filter (Sartolab 150V, vacuum capsule sterile - Sartorius) to separate insoluble parts from the aqueous phase in accordance with the OECD guideline No. 29. The aqueous phase (WAF) was drawn off and used for testing. The control consisted of dilution water only and was not stirred for 48 h.
- Eluate: no
- Differential loading: yes
- Controls: yes, dilution water control
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Obtained from the German Federal Environment Agency, Institut für Wasser-, Boden- und Lufthygiene, Germany. Specimens used in the test were bred in the laboratory at Fraunhofer IME.
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: Adult Daphnia, at least 3 weeks old, were separated from the stock population by sieving. Batches of 30 - 50 animals were held at room temperature in ca. 1.8 L of dilution water for one week. During this week, the daphnids were fed daily with an algal suspension (Desmodesmus subspicatus) and LiquizellR. Algae growing in the log-phase were centrifuged and the pellet was re-suspended in a few mL of medium. Thirty milliliters of this suspension was given to 1 L of medium. The water was changed once per week. Newborn Daphnia (between 4 - 23 h old) were removed by wide-bore pipette (to avoid damage) and isolated in fresh dilution water for at least 1 h prior to being added randomly to the test vessels containing the appropriate test or control media.
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.2 - 20.4 °C
- pH:
- 7.91 - 8.49
- Dissolved oxygen:
- 6.3 - 8.2 mg O2/L
- Nominal and measured concentrations:
- nominal: control, 100 mg/L
measured: < LOQ, 0.128 µg/L (geometric mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel
- Type: covered with a glass pane
- Material, size, headspace, fill volume: glass, 60 mL, headspace: 10 mL, fill volume: 50 mL
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Purified drinking water was used according to the OECD guideline. The purification included filtration with activated charcoal, passage through a lime-stone column and aeration. To avoid copper contamination, plastic water pipes were used for the test facilities. The water was aerated to the point of oxygen saturation.
- Chlorine: < 0.02 mg/L
- Alkalinity: 2 mmol/L
- Conductivity: 244.5 µS/cm
- Nitrate: 1.7 mg/L
- Nitrite: 0.005 mg/L
- Ammonium: < 0.01 mg/L
- Phosphate: 0.73 mg/L
- Culture medium different from test medium: no
- Intervals of water quality measurement: regularly
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light / 8 h dark
- Light intensity: 518 - 536 lux
EFFECT PARAMETERS MEASURED: After 24 h and 48 h, the number of immobile animals in each beaker was counted. The animals were considered to be immobile if they were not able to swim within 15 sec of gentle agitation of the test vessels. Any abnormalities in appearance and behaviour were also recorded.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 10 and 100 mg/L (nominal WAF)
- Results used to determine the conditions for the definitive study: EL50 > 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 0.128 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- > 0.128 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 0.128 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOELR
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- WAF
- Basis for effect:
- mobility
- Details on results:
- - Other biological observations: No signs of disease or stress were observed. All surviving specimens gave the impression of healthy condition.
- Mortality of control: 0% - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50/LC50: EC50 (24 h): 0.87 mg/L
Any other information on results incl. tables
Table1: Measured concentrations of tetradecyl myristate [µg/L].
Nominal loading |
Test duration |
Geometric mean |
||||
Mean measured 0 h |
Mean measured 48 h |
|||||
[mg/L] |
[µg/L] |
% nominal |
[µg/L] |
% nominal |
[µg/L] |
[%] |
100 |
0.172 |
1.72 E-4 |
0.096 |
9.59 E -5 |
0.128 |
1.28 E-4 |
Control |
<LOQ |
- |
<LOQ |
- |
- |
- |
Table 2: Immobility raw data
Geometric mean conc. [mg/L] |
Control |
100 |
0 h |
5 |
5 |
|
5 |
5 |
|
5 |
5 |
|
5 |
5 |
Number of replicates: |
4 |
4 |
Total Introduced: |
20.00 |
20.00 |
24 h |
0 |
0 |
|
0 |
0 |
|
0 |
0 |
|
0 |
0 |
Number of replicates: |
4 |
4 |
Total Immobile: |
0.00 |
0.00 |
48 h |
0 |
0 |
|
0 |
0 |
|
0 |
0 |
|
0 |
0 |
Number of replicates: |
4 |
4 |
Total Immobile: |
0.00 |
0.00 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.