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EC number: 268-594-6 | CAS number: 68130-51-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Remarks:
- in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 April 2014 to 02 July 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study performed in accordance with OECD test guidelines in compliance with GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 014
- Report date:
- 2014
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EPA 40 CFR 158.340
- GLP compliance:
- yes
Test material
- Reference substance name:
- Decanoic acid, mixed esters with heptanoic acid, octanoic acid and trimethylolpropane
- EC Number:
- 268-596-7
- EC Name:
- Decanoic acid, mixed esters with heptanoic acid, octanoic acid and trimethylolpropane
- Cas Number:
- 68130-53-0
- Molecular formula:
- not available due to the complexity of the substance
- IUPAC Name:
- Decanoic acid, heptanoic acid and octanoic acid, mixed esters with trimethylolpropane
- Test material form:
- other: liquid
- Details on test material:
- Identity: H2925 (CAS No 68130-53-0), Lot# 2013090401
Provided by: Chemtura Corporation
Date Received: 18 Mar 2014
Storage: Room temperature and humidity.
Description: Clear yellow liquid
Sample Preparation: Used as received.
Constituent 1
In vitro test system
- Test system:
- human skin model
- Source species:
- human
- Cell type:
- non-transformed keratinocytes
- Cell source:
- other: Not specified
- Source strain:
- not specified
- Details on animal used as source of test system:
- EpiDerm™ tissues, Lot 19981 Kit D, were received from MatTek Corporation (Ashland, MA) on 08 Apr 2014 and refrigerated at approximately 4'C. Before use, tissues were incubated (37°C ± 1°C, 5% ± 1% CO2) with assay medium (MatTek) for a one-hour equilibration. Equilibration medium was replaced with fresh medium before dosing.
- Justification for test system used:
- In accordance with the test guidelines.
- Vehicle:
- unchanged (no vehicle)
- Details on test system:
- Test Article Reduction of MTT: 100 µI of the test article were mixed with 1 ml of MTT solution (1 mg/ml Methyl thiazole tetrazolium diluted in Dulbecco's Modified Eagle's Medium (DMEM)). A Negative Control, 100 µI of tissue culture water, was tested concurrently. The solutions were incubated at room temperature in the dark for 60 minutes. After incubation, the solutions were visually inspected for purple coloration, which indicates that the test article reduced MTT. Since tissue Viability is based on MTT reduction, direct reduction by a test article can exaggerate viability, making a test article seem less irritating than it really is. The test article did not reduce MTT and the assay continued as per the protocol.
Mesh Compatibility: Pre-cut nylon meshes supplied with the tissues were placed on a slide and 30 µI of the test article or tissue culture water were applied. After 60 minutes exposure, the mesh was checked microscopically. No interaction between the test article or tissue culture water and the mesh was observed so the test article was dosed using the mesh as a spreading aid.
Dosing: 50 µI of the test article were applied to the top of each EpiDerm™ tissue. A nylon mesh was then placed on top to facilitate even distribution of the test article. The test article remained in contact with the EpiDerm™ tissue for 3 and 60 minutes. A Negative Control (TCH2O) and a Positive Control (KOH) were tested at 3 and 60 minutes. A nylon mesh was placed on top of each liquid control to facilitate even distribution of the material. Each treatment with test article or control was conducted in duplicate.
All tissues were dosed at ambient temperature. Tissues treated with the test article and controls for 3 minutes were maintained at ambient temperature until analyzed for Tissue Viability (MTT Reduction). Tissues to be treated for 60 minutes were dosed and then returned to the Incubator (37°C ± 1°C, 5% ± 1% CO2) for the remainder of the 60-minute exposure period
Tissue Viability (MTT Reduction): At the end of the exposure period, each EpiDerm™ tissue was rinsed with phosphate buffered saline (PBS) and transferred to a 24-well plate containing 300 µI of MTT solution (1 mg/ml MTT in DMEM). The tissues were then returned to the Incubator for a three-hour MTT incubation period. Following the MTT incubation period, each EpiDerm™ tissue was rinsed with PBS and then treated overnight with 2.0 ml of extractant solution (isopropanol) per well. The absorbency of an aliquot of the extracted MTT formazan was measured In triplicate at 540 nm using a microplate reader (µQuant Plate Reader, BioTek Instruments, Winooski, VT).
Analysis of Data: The mean absorbance value for each time point was calculated from the optical density (OD) of the duplicate tissues and expressed as percent viability for each sample using the following formula:
% viability = 100 X (OD sample/OD Negative Control)
Quality Controls: The Negative Controls meets the acceptance criterion if the mean OD of the two tissues at each time point is greater than or equal to 0.8 and is less than or equal to 2.8 (0.8 ≤ OD ≤ 2.8).
The Positive Control meets the acceptance criterion If the mean relative tissue viability at the 60 minute time point is less than 15% < 15%).
Inter-tissue viability meets the acceptance criterion if the difference between two identically treated tissues is no greater than 30%. Re-testing of the chemical is recommended if the resulting viability is near to a classification cut-off. Note: If necessary, the percent difference will be calculated between the mean of the two tissues and one of the tissues. If this difference is greater than 15%, then rejection should be considered.
Interpretation of Results: Skin Corrosion is defined as the production of irreversible tissue damage in skin following the application of test material. The percent viability calculated was used to determine corrosivity potential in the following manner:
Mean Viability Mean Viability
(3 min.) (60 min.) Predicted Corrosivity
<50% Not Applicable Corrosive
≥50% <15% Corrosive
≥50% ≥15% Non-Corrosive - Control samples:
- yes, concurrent negative control
- yes, concurrent positive control
- Amount/concentration applied:
- 50 µI of the test article were applied to the top of each EpiDerm™ tissue.
- Duration of treatment / exposure:
- 3 and 60 minutes
- Duration of post-treatment incubation (if applicable):
- Not specified
- Number of replicates:
- Tests were repeated in triplicate.
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 3 minute exposure
- Value:
- 85.2
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Irritation / corrosion parameter:
- % tissue viability
- Run / experiment:
- 60 minute exposure
- Value:
- 100
- Vehicle controls validity:
- not applicable
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
EXPERIMENTAL DATA
Test Article: |
H2925 (CAS No. 68130-53-0), Lot# 2013090401 |
||||||
Dose: |
50µl |
||||||
Conc: |
Neat |
||||||
TIME (min) |
OD 1 |
OD 2 |
OD 3 |
MEAN (OD) |
SD |
VIABILITY % |
ERROR % |
3.0 |
1.824 |
1.833 |
1.837 |
1.831 |
0.007 |
89.1 |
0.3 |
|
1.670 |
1.679 |
1.671 |
1.673 |
0.005 |
81.4 |
0.2 |
Neg control |
|
|
|
2.056 |
|
100.0 |
|
60.0 |
1.725 |
1.721 |
1.717 |
1.721 |
0.004 |
89.4 |
0.2 |
|
2.112 |
2.153 |
2.135 |
2.133 |
0.021 |
110.9 |
1.1 |
Neg control |
|
|
|
1.924 |
|
100.0 |
|
Mean % Viability at 3 min: |
85.2 |
|
|||||
Mean % Viability at 60 min: |
100.1 |
|
|||||
Predicted Corrosivity: |
Non-corrosive |
Negative Control: |
Tissue Culture Water |
||||||
Dose: |
50µl |
||||||
Conc: |
Neat |
||||||
TIME (min) |
OD 1 |
OD 2 |
OD 3 |
MEAN (OD) |
SD |
VIABILITY % |
ERROR % |
3.0 |
2.026 |
2.078 |
2.066 |
2.057 |
0.027 |
100.0 |
1.3 |
3.0 |
2.00 |
2.073 |
2.094 |
2.056 |
0.049 |
100.0 |
2.4 |
Mean |
|
|
2.056 |
|
100.0 |
|
|
60.0 |
1.959 |
1.936 |
1.961 |
1.952 |
0.014 |
101.4 |
0.7 |
60.0 |
1.859 |
1.932 |
1.899 |
1.897 |
0.037 |
98.6 |
1.9 |
Mean |
|
|
1.924 |
|
100.0 |
|
|
Predicted Corrosivity: |
Non-corrosive |
Positive Control: |
Potassium Hydroxide, 8.0 N |
||||||
Dose: |
50µl |
||||||
Conc: |
Neat |
||||||
TIME (min) |
OD 1 |
OD 2 |
OD 3 |
MEAN (OD) |
SD |
VIABILITY % |
ERROR % |
3.0 |
0.587 |
0.602 |
0.602 |
0.597 |
0.009 |
29.0 |
0.4 |
3.0 |
0.705 |
0.708 |
0.708 |
0.707 |
0.002 |
34.4 |
0.1 |
Neg control |
|
|
|
2.056 |
|
100.0 |
|
60.0 |
0.038 |
0.039 |
0.040 |
0.039 |
0.001 |
2.0 |
0.1 |
60.0 |
0.043 |
0.043 |
0.043 |
0.043 |
0.000 |
2.2 |
0.0 |
Neg control |
|
|
|
1.924 |
|
100.0 |
|
Mean % Viability at 3 min: |
31.7 |
|
|||||
Mean % Viability at 60 min: |
2.1 |
|
|||||
Predicted Corrosivity: |
Corrosive |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- Criteria used for interpretation of results: EU
- Conclusions:
- H2925 (CAS No. 68130-53-0), Lot# 2013090401 is predicted to be non-corrosive.
- Executive summary:
OBJECTIVE: To predict and classify the skin corrosivity potential of a chemical by using a three-dimensional human epidermis model. This study is designed based on MatTek protocol "In Vitro EpiDerm™ Skin Corrosion Test" and follows the OECD Guideline for the Testing of Chemicals No. 431 In Vitro Skin Corrosion: Human Skin Model Test.
METHOD SYNOPSIS: MatTek EpiDermTMtissues were treated in duplicate with the test article. Negative Control and Positive Control for 3 minutes and 60 minutes. Following treatment, the viability of thetissues was determined using Methyl thiazole tetrazolium (MTT) uptake and conversion, and theabsorbance of each tissue was measured at 540 nm. The mean viability was then expressed as apercent of control values. The percent viability was used to determine corrosivity potential.
SUMMARY/CONCLUSION:
Test and Control Article Identity
Mean Viability (3 min.)
Mean Viability (60 min.)
Predicted Corrosivity
H2925 (CAS No. 68130-53-0), Lot# 2013090401
85.2%
100.1%
Non-Corrosive
Tissue culture water (Negative Control)
100.0%
100.0%
Non-Corrosive
Potassium Hydroxide, 8.0 N (Positive Control)
31.7%
2.1%
Corrosive
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