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EC number: 263-034-7 | CAS number: 61789-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09. Apr. 2018 to 13. Apr. 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- OECD Guideline for the Testing of Chemicals No. 201, adopted 23. Mar. 2006, Annex 5 corrected: 28 July 2011 “Freshwater Alga and Cyanobacteria, Growth Inhibition Test”
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Regulation EU No. 2016/266 amending Regulation EC No. 440/2008, Annex IV, Method C.3: “FRESHWATER ALGAE AND CYANOBACTERIA, GROWTH INHIBI-TION TEST,” adopted 07. December 2015
- Deviations:
- yes
- Remarks:
- See "Any other information" for details
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- No further details specified in the study report.
- Analytical monitoring:
- yes
- Details on sampling:
- The content of DOC in the test vessels was measured at the start and at the end of the test.
- Vehicle:
- no
- Details on test solutions:
- The water-accommodated fraction (WAF) was prepared for the test. This was done by mixing 99.75 mg/L of the test item with the corresponding amount of algal medium (de-mineralised water enriched with minerals but without algae) and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 μm PTFE filters. The lower treat-ments were prepared by dilution of this WAF with nutrient medium. This procedure is in agreement with the OECD guidance document no. 23 for the testing of mixtures which are toxic at very low loading rates. In a non-GLP pre-test at the loading rate 1 mg/L clear inhi-bition of algal growth was observed.
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- Specification
Unicellular: freshwater green alga.
Genus: Desmodesmus
Species: subspicatus SAG Strain Number 86.81
Taxonomic position: Chlorophyta - Chlorophyceae
Origin and Culture
The culture of Desmodesmus subspicatus was obtained in January 2016 by MBM Sci-encebridge GmbH (Institut für Pflanzenphysiologie of Universität Göttingen). The algae are kept as stock culture on solid agar at 2 - 8 °C. From the stock culture, a permanent culture was prepared. From an aliquot of the permanent culture, the pre-culture was prepared. - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- Not specified
- Hardness:
- Not specified
- Test temperature:
- 21.8 – 23.5 °C
- pH:
- 7.5 - 7.9
- Dissolved oxygen:
- Not specified
- Salinity:
- Not applicable - freshwater study
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- 1, 3.2, 10, 32, 100 mg/L nominal concentration
The concentrations to be tested based on non GLP pre-tests - Details on test conditions:
- Pre-Culture
Four days before the start of each test, an aliquot of the permanent culture was brought into nutrient medium and incubated under continuous lighting for 96 hours under test con-ditions (5000 lux, 21.7 – 23.1°C). The resulting culture grew exponentially.
Before usage, the pre-culture was checked for the absence of cell aggregates and the cell number of culture was determined.
Performance of the Study
For each treatment, 200 mL of the respective test item solution was mixed with the neces-sary amount of algal pre-culture (0.363 mL) to achieve a cell concentration of 2.0*103 cells/mL. For the blank control, 350 mL nutrient medium was used instead of test item so-lution and mixed with the necessary amount of algal pre-culture (0.635 mL).
The real cell concentration at the beginning of the test was measured with an electronic particle counter in the blank control solution. This measured value was used as start cell concentration for all replicates.
From these mixtures samples for the analytical determination were taken, afterwards the pH-value was measured.
The test vessels were filled with 45 ± 1 mL of the respective test solution and incubated open (covered with perforated plastic foil acting as a stopper) for 72 hours, shaken on an orbital shaker to keep the algae in suspension. Before the start of incubation and every 24 ± 1 hours, the cell number was determined with an electronic particle counter. After the test, the pH value in treatments and blank control was measured again.
At the end of the test, the treatments were examined microscopically in order to assess the appearance of the algae and detect abnormalities (e.g. caused by the exposure to the test item).
The content of DOC in the test vessels was measured at the start and at the end of the test.
Experimental Conditions
Treatments tested: 1 / 3.2 / 10 / 32 / 100 mg/L nominal concentration. The concentrations to be tested based on non GLP pre-tests
Number of replicates: 6 replicates for the blank control 3 replicates for each treatment
Vessels: glass flasks total volume 65 mL
Duration: 72 hours
Lighting: 5000 lux
Temperature: 21.8 – 23.5 °C
Replicates (Blank control): 45 ±1 mL algal medium and algae
Replicates (Treatments): 45 ±1 mL test solution and algae - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate & yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: growht rate & yield
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 43.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: yield
- Details on results:
- DISCUSSION
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 1 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate μ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
10 – 100 mg/L
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solu-tions using a carbon analyser.
The test item could only be detected in the highest concentration. All other measured values are approximately in the same range as the blank control. However, due to the clear dose-response relationship, it is clear that dissolved test item was present in solution.
Therefore, the statistical evaluation was carried out with the nominal concentrations. - Results with reference substance (positive control):
- 72h ErC50: 0.90 mg/L
72h EyC50: 0.42 mg/L - Reported statistics and error estimates:
- Calculation of results was performed with the help of validated software (Microsoft Ex-cel®). The estimation of the biological data was accomplished using the software ToxRat® Professional, version 3.2.1.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following results for the test item LUMULSE GMT-K were determined:
NOEC LOEC EC10 EC50
Growth Rate 3.2 mg/L 10 mg/L 35.79 mg/L > 100 mg/L
Yield 3.2 mg/L 10 mg/L 9.76 mg/L 43.60 mg/L - Executive summary:
Determination of the toxicity of LUMULSE GMT-K against Desmodesmus subspicatus according to OECD 201 resp. EU C.3
Findings and Results:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 1 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate μ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
10 – 100 mg/L
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solu-tions using a carbon analyser.
The test item could only be detected in the highest concentration. All other measured values are approximately in the same range as the blank control. However, due to the clear dose-response relationship, it is clear that dissolved test item was present in solution.
Therefore, the statistical evaluation was carried out with the nominal concentrations (cf. OECD Guideline No. 201, paragraph 40, page 6).
Since the test item is an UVCB, the EC50 value (growth rate) calculated by ToxRat® by extrapolation is not given but a range.
The 72h-EC50 values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test item LUMULSE GMT-K were determined:
Results of the test item
Endpoint
NOEC
LOEC
EC10
EC50
Growth Rate
3.2 mg/L
10 mg/L
35.79 mg/L
> 100 mg/L
Yield
3.2 mg/L
10 mg/L
9.76 mg/L
43.60 mg/L
Reference
Biological Results of K2Cr2O7
Parameter |
Value |
95% confidence interval |
72h ErC50 |
0.90 mg/L |
0.77 – 1.1 mg/L |
72h EyC50 |
0.42 mg/L |
0.35 – 0.51 mg/L |
Cell Numbers
The cell numbers were determined with an electronic particle counter. The means and standard deviations of the cell numbers of the blank control and the treatments are presented in the following table:
Cell Number/mL
Nominal Concentration in mg/L |
Parameter |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
Mean |
2000 |
10333 |
61580 |
294913 |
Blank control |
SD |
0 |
1244 |
8677 |
21154 |
1 |
Mean |
2000 |
9927 |
48080 |
308387 |
1 |
SD |
0 |
794 |
8254 |
13710 |
3.2 |
Mean |
2000 |
8213 |
34447 |
279313 |
3.2 |
SD |
0 |
1435 |
2311 |
45673 |
10 |
Mean |
2000 |
8520 |
42673 |
246220 |
10 |
SD |
0 |
225 |
2706 |
22986 |
32 |
Mean |
2000 |
9433 |
30933 |
197673 |
32 |
SD |
0 |
1417 |
1363 |
26872 |
100 |
Mean |
2000 |
5633 |
21553 |
59227 |
100 |
SD |
0 |
376 |
1389 |
8584 |
SD = Standard Deviation
Light Intensity
In the following table, the light intensity during the test is stated:
Light Intensity
|
0 h |
24 h |
48 h |
72 h |
Light intensity [Lux] |
5000 |
5000 |
5000 |
5000 |
pH values
In the following table, the pH values measured at the start and end of the test are stated:
pH values
Nominal Concentration in mg/L |
0 h |
72 h |
Blank control |
7.6 |
7.7 |
1 |
7.7 |
7.9 |
3.2 |
7.6 |
7.8 |
10 |
7.6 |
7.6 |
32 |
7.7 |
7.5 |
100 |
7.6 |
7.5 |
Microscopical Observations
In the following table, the appearance of the algae at the end of the test is stated:
Microscopical Observations
Nominal Concentration in mg/L |
Normal and Healthy Appearance of the Algae |
Blank control |
Yes |
1 |
Yes |
3.2 |
Yes |
10 |
Yes |
32 |
Yes |
100 |
Yes |
Analytical Determination
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
The test item could only be detected in the highest concentration. All other measured values are approximately in the same range as the blank control. However, due to the clear dose-response relationship, it is clear that dissolved test item was present in solution.
Therefore, the statistical evaluation was carried out with the nominal concentrations.
The details are given in the following tables:
Measured Concentrations IC and TC
Nominal Concentration Test Item |
Measured TC t = 0 h |
Measured TC t = 72 h |
Measured IC t = 0 h |
Measured IC t = 72 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
7.30 |
7.00 |
6.84 |
6.21 |
1 |
7.44 |
6.64 |
7.01 |
6.08 |
3.2 |
7.64 |
6.58 |
7.00 |
6.09 |
10 |
8.24 |
6.34 |
6.87 |
5.77 |
32 |
9.89 |
6.75 |
7.05 |
5.74 |
100 |
15.78 |
7.38 |
6.71 |
5.87 |
LOQ (Limit of quantification) TC = 5.43 mg/L
LOQ (Limit of quantification) IC = 2.03 mg/L
Measured Concentrations DOC
Nominal Concentration Test Item |
Measured DOC (TC-IC) t = 0 h |
Measured DOC (TC-IC) t = 72 h |
Measured DOC minus blank control t = 0 h |
Measured DOC minus blank control t = 72 h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
0.46 |
0.79 |
-- |
-- |
1 |
0.44 |
0.55 |
-0.03 |
-0.23 |
3.2 |
0.64 |
0.49 |
0.18 |
-0.29 |
10 |
1.37 |
0.57 |
0.91 |
-0.22 |
32 |
2.84 |
1.01 |
2.38 |
0.22 |
100 |
9.06 |
1.51 |
8.60 |
0.72 |
Calculated Test Item Concentrations
Nominal Concentration Test Item |
Calculated Concentration t = 0 h |
Calculated Concentration t = 72 h |
% of Nominal concentration t = 0 h |
% of Nominal concentration t = 72h |
mg/L |
mg/L |
mg/L |
mg/L |
mg/L |
Blank control |
-- |
-- |
-- |
-- |
1 |
0.0 |
-0.3 |
-4 |
-32 |
3.2 |
0.3 |
-0.4 |
8 |
-13 |
10 |
1.3 |
-0.3 |
13 |
-3 |
32 |
3.3 |
0.3 |
10 |
1 |
100 |
12.1 |
1.0 |
12 |
1 |
Growth Rate, Yield
From the cell numbers, the Growth Rate μ and the Yield were calculated. The means and standard deviations at the end of the test are given in the following table:
Growth Rate μ, Yield
Nominal Concentration in mg/L |
Parameter |
Growth rate (0-72h) [day-1] |
Yield (0-72h) [Cell Concentration/mL] |
Blank control |
Mean |
1.66 |
292913 |
SD |
0.02 |
21154 |
|
1 |
Mean |
1.68 |
306387 |
SD |
0.01 |
13710 |
|
3.2 |
Mean |
1.64 |
277313 |
SD |
0.06 |
45673 |
|
10 |
Mean |
1.60 |
244220 |
SD |
0.03 |
22986 |
|
32 |
Mean |
1.53 |
195673 |
SD |
0.04 |
26872 |
|
100 |
Mean |
1.13 |
57227 |
SD |
0.05 |
8584 |
SD = Standard Deviation
Inhibition
The following mean inhibition values were calculated for the Growth Rate μ and the Yield.
Inhibition Values
Nominal concentration in mg/L |
% Inhibition |
|
Growth Rate (0-72h) |
Yield (0-72h) |
|
Blank control |
0 |
0 |
1 |
-0.9 |
-4.6 |
3.2 |
1.2 |
5.3 |
10 |
3.6 |
16.6 |
32 |
8.1 |
33.2 |
100 |
32.3 |
80.5 |
Negative inhibition values indicate a stimulation of alga growth compared to the blank control.
Measured Cell Numbers
The measured cell numbers for each replicate are presented in the following table:
Cell numbers measured
Nominal concentration in mg/L |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
|
Blank control |
100 |
447 |
3641 |
13372 |
100 |
524 |
2713 |
13672 |
|
100 |
573 |
2722 |
14548 |
|
100 |
445 |
3601 |
15599 |
|
100 |
595 |
3013 |
15340 |
|
100 |
516 |
2784 |
15943 |
|
1 |
100 |
542 |
2830 |
16105 |
100 |
477 |
2376 |
15419 |
|
100 |
470 |
2006 |
14734 |
|
3.2 |
100 |
491 |
1718 |
15550 |
100 |
353 |
1609 |
14999 |
|
100 |
388 |
1840 |
11348 |
|
10 |
100 |
433 |
1979 |
10984 |
100 |
432 |
2230 |
12962 |
|
100 |
413 |
2192 |
12987 |
|
32 |
100 |
453 |
1501 |
9126 |
100 |
550 |
1514 |
9090 |
|
100 |
412 |
1625 |
11435 |
|
100 |
100 |
291 |
1087 |
2933 |
100 |
260 |
1004 |
2547 |
|
100 |
294 |
1142 |
3404 |
Calculated Cell Numbers
The cell number in the solution were calculated (dilution factor 20). The single values and the corresponding means and standard deviations are presented in the following table:
Cells Numbers calculated
Nominal Conc. in mg/L |
Replicate |
Cell Number/mL |
|||
0 h |
24 h |
48 h |
72 h |
||
Blank control |
1 |
2000 |
8940 |
72820 |
267440 |
2 |
2000 |
10480 |
54260 |
273440 |
|
3 |
2000 |
11460 |
54440 |
290960 |
|
4 |
2000 |
8900 |
72020 |
311980 |
|
5 |
2000 |
11900 |
60260 |
306800 |
|
6 |
2000 |
10320 |
55680 |
318860 |
|
Mean |
2000 |
10333 |
61580 |
294913 |
|
SD |
0 |
1244 |
8677 |
21154 |
|
1 |
1 |
2000 |
10840 |
56600 |
322100 |
2 |
2000 |
9540 |
47520 |
308380 |
|
3 |
2000 |
9400 |
40120 |
294680 |
|
Mean |
2000 |
9927 |
48080 |
308387 |
|
SD |
0 |
794 |
8254 |
13710 |
|
3.2 |
1 |
2000 |
9820 |
34360 |
311000 |
2 |
2000 |
7060 |
32180 |
299980 |
|
3 |
2000 |
7760 |
36800 |
226960 |
|
Mean |
2000 |
8213 |
34447 |
279313 |
|
SD |
0 |
1435 |
2311 |
45673 |
|
10 |
1 |
2000 |
8660 |
39580 |
219680 |
2 |
2000 |
8640 |
44600 |
259240 |
|
3 |
2000 |
8260 |
43840 |
259740 |
|
Mean |
2000 |
8520 |
42673 |
246220 |
|
SD |
0 |
225 |
2706 |
22986 |
|
32 |
1 |
2000 |
9060 |
30020 |
182520 |
2 |
2000 |
11000 |
30280 |
181800 |
|
3 |
2000 |
8240 |
32500 |
228700 |
|
Mean |
2000 |
9433 |
30933 |
197673 |
|
SD |
0 |
1417 |
1363 |
26872 |
|
100 |
1 |
2000 |
5820 |
21740 |
58660 |
2 |
2000 |
5200 |
20080 |
50940 |
|
3 |
2000 |
5880 |
22840 |
68080 |
|
Mean |
2000 |
5633 |
21553 |
59227 |
|
SD |
0 |
376 |
1389 |
8584 |
SD = Standard Deviation
Growth Rate μ
The growth rate μ for each replicate and the corresponding means and standard deviation are given in the following table:
Growth Rate μ
Nominal Conc. in mg/L |
Replicate |
Growth Rate μ [day-1] |
||
24 h |
48 h |
72 h |
||
Blank control |
1 |
1.50 |
1.80 |
1.63 |
2 |
1.66 |
1.65 |
1.64 |
|
3 |
1.75 |
1.65 |
1.66 |
|
4 |
1.49 |
1.79 |
1.68 |
|
5 |
1.78 |
1.70 |
1.68 |
|
6 |
1.64 |
1.66 |
1.69 |
|
Mean |
1.64 |
1.71 |
1.66 |
|
SD |
0.12 |
0.07 |
0.02 |
|
1 |
1 |
1.69 |
1.67 |
1.69 |
2 |
1.56 |
1.58 |
1.68 |
|
3 |
1.55 |
1.50 |
1.66 |
|
Mean |
1.60 |
1.58 |
1.68 |
|
SD |
0.08 |
0.09 |
0.01 |
|
3.2 |
1 |
1.59 |
1.42 |
1.68 |
2 |
1.26 |
1.39 |
1.67 |
|
3 |
1.36 |
1.46 |
1.58 |
|
Mean |
1.40 |
1.42 |
1.64 |
|
SD |
0.17 |
0.03 |
0.06 |
|
10 |
1 |
1.47 |
1.49 |
1.57 |
2 |
1.46 |
1.55 |
1.62 |
|
3 |
1.42 |
1.54 |
1.62 |
|
Mean |
1.45 |
1.53 |
1.60 |
|
SD |
0.03 |
0.03 |
0.03 |
|
32 |
1 |
1.51 |
1.35 |
1.50 |
2 |
1.70 |
1.36 |
1.50 |
|
3 |
1.42 |
1.39 |
1.58 |
|
Mean |
1.54 |
1.37 |
1.53 |
|
SD |
0.15 |
0.02 |
0.04 |
|
100 |
1 |
1.07 |
1.19 |
1.13 |
2 |
0.96 |
1.15 |
1.08 |
|
3 |
1.08 |
1.22 |
1.18 |
|
Mean |
1.03 |
1.19 |
1.13 |
|
SD |
0.07 |
0.03 |
0.05 |
SD = Standard Deviation
Yield
The Yield for each replicate and the corresponding means and standard deviations are given in the following table:
Yield
Nominal Conc. in mg/L |
Replicate |
Yield [Cell Concentration/mL] |
72 h |
||
Blank control |
1 |
265440 |
2 |
271440 |
|
3 |
288960 |
|
4 |
309980 |
|
5 |
304800 |
|
6 |
316860 |
|
Mean |
292913 |
|
SD |
21154 |
|
1 |
1 |
320100 |
2 |
306380 |
|
3 |
292680 |
|
Mean |
306387 |
|
SD |
13710 |
|
3.2 |
1 |
309000 |
2 |
297980 |
|
3 |
224960 |
|
Mean |
277313 |
|
SD |
45673 |
|
10 |
1 |
217680 |
2 |
257240 |
|
3 |
257740 |
|
Mean |
244220 |
|
SD |
22986 |
|
32 |
1 |
180520 |
2 |
179800 |
|
3 |
226700 |
|
Mean |
195673 |
|
SD |
26872 |
|
100 |
1 |
56660 |
2 |
58940 |
|
3 |
66080 |
|
Mean |
67227 |
|
SD |
8584 |
SD = Standard Deviation
Inhibition Values of Single Replicates
The inhibition values at the end of the test of the single replicates are presented in the following table:
Inhibition Values at the end of the test (72 hours)
Nominal Conc. in mg/L |
Replicate |
Inhibition Growth Rate μ 72 hours |
Inhibition Yield 72 hours |
Blank control |
1 |
1.92 |
9.38 |
2 |
1.47 |
7.33 |
|
3 |
0.23 |
1.35 |
|
4 |
-1.17 |
-5.83 |
|
5 |
-0.84 |
-4.06 |
|
6 |
-1.61 |
-8.18 |
|
Mean |
0 |
0 |
|
SD |
1.45 |
7.22 |
|
1 |
1 |
-1.81 |
-9.28 |
2 |
-0.94 |
-4.60 |
|
3 |
-0.03 |
0.08 |
|
Mean |
-0.93 |
-4.60 |
|
SD |
0.89 |
4.68 |
|
3.2 |
1 |
-1.11 |
-5.49 |
2 |
-0.38 |
-1.73 |
|
3 |
5.20 |
23.20 |
|
Mean |
1.24 |
5.33 |
|
SD |
3.45 |
15.59 |
|
10 |
1 |
5.86 |
25.68 |
2 |
2.54 |
12.18 |
|
3 |
2.50 |
12.01 |
|
Mean |
3.63 |
16.62 |
|
SD |
1.93 |
7.85 |
|
32 |
1 |
9.57 |
38.37 |
2 |
9.65 |
38.62 |
|
3 |
5.05 |
22.61 |
|
Mean |
8.09 |
33.20 |
|
SD |
2.63 |
9.17 |
|
100 |
1 |
32.31 |
80.66 |
2 |
35.14 |
83.29 |
|
3 |
29.33 |
77.44 |
|
Mean |
32.26 |
80.46 |
|
SD |
2.91 |
2.93 |
SD = Standard Deviation
Description of key information
The following results for the test item LUMULSE GMT-K were determined:
Results of the test item
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
3.2 mg/L |
10 mg/L |
35.79 mg/L |
> 100 mg/L |
Yield |
3.2 mg/L |
10 mg/L |
9.76 mg/L |
43.60 mg/L |
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 43.6 mg/L
- EC10 or NOEC for freshwater algae:
- 3.2 mg/L
Additional information
The study was performed using 5 concentrations ranging from 1 to 100 mg/L. Incubation time (test system Desmodesmus subspicatus) was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate μ and the yield1 were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations: 10 – 100 mg/L
At the start and at the end of the test, the content of the test item in the test solutions was estimated by determination of the dissolved organic carbon (DOC) content in the test solutions using a carbon analyser.
The test item could only be detected in the highest concentration. All other measured values are approximately in the same range as the blank control. However, due to the clear dose-response relationship, it is clear that dissolved test item was present in solution.
Therefore, the statistical evaluation was carried out with the nominal concentrations.
Since the test item is an UVCB, the EC50 value (growth rate) calculated by ToxRat® by extrapolation is not given but a range.
The following results for the test item LUMULSE GMT-K were determined:
Results of the test item
Endpoint |
NOEC |
LOEC |
EC10 |
EC50 |
Growth Rate |
3.2 mg/L |
10 mg/L |
35.79 mg/L |
> 100 mg/L |
Yield |
3.2 mg/L |
10 mg/L |
9.76 mg/L |
43.60 mg/L |
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