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EC number: 240-566-8 | CAS number: 16499-88-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- other:
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 05 March 2018 - 17 April 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3200 (Zahn-Wellens / EMPA Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Identification: BOPA
- CAS Number: 16499-88-0
- Chemical Name: 3-Butoxypropylamine
- Physical state/Appearance: clear colorless liquid
- Batch: PFW160573
- Purity: 99.39%
- Molecular Weight: 131.22 g/mol
- Receipt Date: 04 May 2017
- Expiry Date: 26 September 2018
- Storage Conditions: room temperature, in the dark - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK (Aeration stage)
- Laboratory culture: maintained on continuous aeration upon receipt
- Method of cultivation: Activated sewage sludge was washed twice by settlement and resuspension in mineral medium to remove any excessive amounts of Dissolved Organic Carbon (DOC). A sub-sample of the washed sewage sludge was then removed and the suspended solids concentration determined.
- Initial cell/biomass concentration: 300 mg suspended solids/liter in order to give an inoculum to carbon ratio of 3:1.
- Water filtered: yes
- Type and size of filter used, if any: Gelman 0.45 µm AcroCap filter - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 100 other: mg carbon/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Nutrient Stock Solutions
Solution a KH2PO4: 8.50 g/L
K2HPO4: 21.75 g/L
Na2HPO4.2H2O: 33.40 g/L
NH4Cl: 0.50 g/L
pH = 7.4
Solution b CaCl2: 27.50 g/L
Solution c MgSO4.7H2O: 22.50 g/L
Solution d FeCl3.6H2O: 0.25 g/L
(In order to avoid having to prepare solution (d) immediately before use, one drop of concentrated HCl per liter was added as a preservative).
Preparation of mineral medium for washing the activated sewage sludge
To 10 liters (final volume) of purified water* was added the following:
100 mL of Solution a
10 mL of Solution b
10 mL of Solution c
10 mL of Solution d
Preparation of mineral medium for biodegradation test
To approximately 500 mL of purified water* was added the following:
20 mL of Solution a
2 mL of Solution b
2 mL of Solution c
2 mL of Solution d
Activated sludge to give 300 mg suspended solids per liter final volume.
The volume was then adjusted to 2 liters using purified water after the addition of test item and/or reference item if applicable.
(*Ion exchange and reverse osmosis treated tap water (Elga Optima 15+ or Elga Purelab Option – R15 BP))
- Test temperature: 20 - 25°C
- pH: 7 - 8
- pH adjusted: yes
- Aeration of dilution water: Constantly aerated with compressed air via glass tubes and stirred constantly by a magnetic stirrer.
- Suspended solids concentration: 300 mg suspended solids/liter
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration:
* inoculum control, duplicate
* reference item (diethylene glycol), duplicate
* test item, duplicate
* test item plus diethylene glycol, one replicate
- Method used to create aerobic conditions: continuous aeration
- Method used to create anaerobic conditions: n/a
- Measuring equipment: Shimadzu TOC-VCPH TOC analyzer
- Test performed in closed vessels due to significant volatility of test substance: no
SAMPLING
- Sampling frequency: Samples taken for analysis at 0 and 3 hours and on Days 1, 7, 14, 21, 27 and 28.
- Sampling method: Samples (approximately 30 mL) were filtered through 0.45 µm Gelman AcroCap disposable filters. The first approximate 5 mL of filtrate was discarded.
CONTROL AND BLANK SYSTEM
- Inoculum blank: consisting of inoculated mineral medium
- Abiotic sterile control: n/a
- Toxicity control: containing the test item and diethylene glycol
- Other: not specified
- Reference substance:
- diethylene glycol
- Preliminary study:
- The preliminary investigation indicated that the test item did not adsorb to filter matrices or activated sewage sludge. Therefore, for the purpose of the test, the samples taken for DOC analysis were filtered to remove the suspended solids present without causing a loss of any test item.
- Test performance:
- Diethylene glycol attained 99% biodegradation after 14 days and 100% biodegradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions.
The toxicity control attained 98% biodegradation after 14 days and 99% biodegradation after 28 days, therefore confirming that the test item was not toxic to the sewage treatment micro-organisms used in the study. - Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 11
- St. dev.:
- 0
- Sampling time:
- 1 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 15
- St. dev.:
- 0
- Sampling time:
- 7 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 98
- St. dev.:
- 0
- Sampling time:
- 14 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 99
- St. dev.:
- 0
- Sampling time:
- 21 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 99
- St. dev.:
- 0
- Sampling time:
- 27 d
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- ca. 99
- St. dev.:
- 0
- Sampling time:
- 28 d
- Details on results:
- The procedure control, diethylene glycol attained 99% biodegradation after 14 days and 100% biodegradation after 28 days, thereby confirming the suitability of the inoculum and culture conditions.
The toxicity control attained 98% biodegradation after 14 days and 99% biodegradation after 28 days, therefore confirming that the test item was not toxic to the sewage treatment micro-organisms used in the study.
The pH values did not fall below 6.61 in any test vessel during the study and were readjusted to pH 7 to 8 where necessary on sampling days. Aerobic conditions in the test vessels were maintained throughout the study as dissolved oxygen concentrations remained at or above 7.01 mg O2/L in all culture vessels.
Table 1 shows the results of the percentage biodegradation. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- From DOC analysis the test item attained 98% biodegradation after 14 days and 99% biodegradation after 28 days exposure time. Between days 14 and 28 the biodegradation rate reached a plateau.
OECD Guideline No. 302B does not give any definitive pass levels for test items, however the “Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3 (2006)” suggests that a figure of more than 20% biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70% mineralization may be regarded as evidence for ultimate inherent biodegradation.
The test item can therefore be considered to have exhibited evidence of ultimate biodegradation under the experimental conditions employed in this study.
Referenceopen allclose all
Table 1. Percentage biodegradation
Day |
% Biodegradation
|
||
Procedure Control |
Test Item |
Toxicity Control |
|
1 |
9 |
11 |
12 |
7 |
99 |
15 |
78 |
14 |
99 |
98 |
98 |
21 |
99 |
99 |
99 |
27 |
99 |
99 |
99 |
28 |
100 |
99 |
99 |
Description of key information
One study (Covance, 2019) is included in this dossier and regarded as a key study (Klimisch score of 1). The biodegradability of 3-butoxypropan-1-amine was determined according to OECD 302B, EU Method C.9 and EPA OPPTS 835.3110. From DOC analysis the test item attained 98% biodegradation after 14 days and 99% biodegradation after 28 days exposure time. Between days 14 and 28 the biodegradation rate reached a plateau. OECD Guideline No. 302B does not give any definitive pass levels for test items, however the “Revised Introduction to the OECD Guidelines for Testing of Chemicals, Section 3 (2006)” suggests that a figure of more than 20% biodegradation may be regarded as evidence for inherent, primary biodegradability. A figure of more than 70% mineralization may be regarded as evidence for ultimate inherent biodegradation. The test item can therefore be considered to have exhibited evidence of ultimate biodegradation under the experimental conditions employed in this study.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
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