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EC number: 258-548-3 | CAS number: 53423-65-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 25 - February 12, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 420 (Acute Oral Toxicity - Fixed Dose Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 bis (Acute Oral Toxicity - Fixed Dose Procedure)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- fixed dose procedure
- Limit test:
- yes
Test material
- Reference substance name:
- Sodium 2,4,5-trichlorobenzenesulphonate
- EC Number:
- 258-548-3
- EC Name:
- Sodium 2,4,5-trichlorobenzenesulphonate
- Cas Number:
- 53423-65-7
- Molecular formula:
- C6H2Cl3O3S.Na
- IUPAC Name:
- sodium 2,4,5-trichlorobenzenesulfonate
- Test material form:
- solid: particulate/powder
Constituent 1
- Specific details on test material used for the study:
- Composition: STB-FR (Benzenesulfonic acid, 2,4,5-trichloro-, sodium salt)
Lot #: 1710-06
Purity: 90.5%
CAS# 53423-65-7
EC# 258-548-3
Physical Description: White powder
pH: 7.11
Stability: Test substance was expected to be stable for the duration of testing.
Expiration Date: October 2019
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- The animals were singly housed in suspended stainless steel caging, which conforms to the size recommendations in the most recent Guide for the Care and Use of Laboratory Animals (Natl. Res. Council, 2011). Enrichment (e.g., toy) was placed in each cage. Litter paper was placed beneath the cage and was changed at least three times per week. Temperature and relative humidity ranges were kept at 19-26ºC and 38-58% humidity, with a 12 hour light/dark cycle. The animals were fed, Envigo Teklad Global 16% Protein Rodent Diet® and filtered tap water. Available ad libitum.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- The test substance, as received, was a powder. The test substance was administered as a 40% w/w mixture in distilled water. Preliminary sample preparation assessments conducted by PSL indicated that mixtures in excess of 40% (i.e., 50-80%) were too viscous to be administered properly. Each preparation was mixed well prior to use.
- Doses:
- Individual doses were calculated based on the initial body weights, taking into account the density and concentration of the test mixture
- No. of animals per sex per dose:
- 5 female rats, given one dose of 2000 mg/kg each.
- Control animals:
- no
- Details on study design:
- The animals were observed for mortality, signs of gross toxicity, and behavioral changes approximately 30 minutes post-dosing, during the first several hours post-dosing and at least once daily thereafter for 14 days after dosing. Observations included gross evaluation of skin and fur, eyes and mucous membranes, respiratory, circulatory, autonomic and central nervous systems, somatomotor activity and behavior pattern. Particular attention was directed to observation of tremors, convulsions, salivation, diarrhea, and coma.
- Statistics:
- Statistical analysis was limited to the calculation of the mean density value for dosing.
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All rats survived testing and the 14-day observation period.
- Clinical signs:
- other: Apart from one female exhibiting irregular respiration for the first three hours following administration, there were no other signs of gross toxicity, adverse clinical effects, or abnormal behavior.
- Gross pathology:
- No gross abnormalities were noted for any of the animals necropsied at the conclusion of the 14-day observation period.
- Other findings:
- None
Any other information on results incl. tables
INDIVIDUAL BODY WEIGHTS AND DOSES:
Animal No. |
Sex |
Dose Level (mg/kg) |
Body Weight (g) |
Dose1 |
||
Initial |
Day 7 |
Day 14 |
mL |
|||
3101 |
F |
2000 |
183 |
228 |
238 |
0.75 |
3102 |
F |
200 |
240 |
255 |
0.82 |
|
3103 |
F |
197 |
228 |
245 |
0.81 |
|
3104 |
F |
194 |
219 |
254 |
0.80 |
|
3105 |
F |
189 |
226 |
246 |
0.77 |
1The test substance was administeredas a 40% w/w mixture indistilled water. Density –1.220g/mL.
INDIVIDUAL CAGE-SIDE OBSERVATIONS:
Animal Number |
Animal Sex |
Dose Level (mg/kg) |
Observation |
Day of Observation (x=observation is present) |
|||||||||||||||
0(0.5 hr) |
0(3 hrs) |
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
13 |
14 |
||||
3101 |
F |
2000 |
Irregular respiration |
x |
x |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Active and healthy |
|
|
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
|||
|
|||||||||||||||||||
3102 |
F |
2000 |
Active and healthy |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
|
|||||||||||||||||||
3103 |
F |
2000 |
Active and healthy |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
|
|||||||||||||||||||
3104 |
F |
2000 |
Active and healthy |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
|
|||||||||||||||||||
3105 |
F |
2000 |
Active and healthy |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
x |
INDIVIDUAL NECROPSY OBSERVATIONS:
Animal Number |
Animal Sex |
Dose Level (mg/kg) |
Organ / Tissue |
Observation |
3101 |
F |
2000 |
All tissues and organs |
No gross abnormalities |
|
||||
3102 |
F |
2000 |
All tissues and organs |
No gross abnormalities |
|
||||
3103 |
F |
2000 |
All tissues and organs |
No gross abnormalities |
|
||||
3104 |
F |
2000 |
All tissues and organs |
No gross abnormalities |
|
||||
3105 |
F |
2000 |
All tissues and organs |
No gross abnormalities |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of this study, the acute oral LD50 of the substance is greater than (>) 2000 mg/kg of body weight in female rats.
- Executive summary:
In a GLP guideline study conducted according to OPPTS 870.1100, OECD Test No. 420, and EC method B.1 using the Acute Oral Fixed Dose Procedure, the acute oral LD50 of the test substance was determined to be greater than (>) 2000 mg/kg of body wieght in female Sprague-Dawley rats. All animals survived test substance administration and gained body weight during the study. Apart from one female exhibiting irregular respiration for the first three hours following administration, there were no other signs of gross toxicity, adverse clinical effects, or abnormal behavior. No gross abnormalities were noted for any of the animals when necropsied at the conclusion of the 14-day observation period.
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