Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 916-603-5 | CAS number: -
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of Basic Red 76 to the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201 and Council Regulation (EC) No. 266/2016/Method C.3.The aim of the study was the determination of NOEC, LOEC, EC10-, EC20- and EC50-values of growth rate and yield over a period of 72 hours. The study was conducted under static conditions with an initial cell density of 5523 cells/mL. A stock solution of 10.0 mg/L was prepared with dilution water. Five concentrations in a geometrical series with a dilution factor of 2 were tested (nominal) : 0.625 - 1.25 - 2.50 - 5.00 - 10.0 mg/L, corresponding to the geometric mean test item concentrations 0.329 - 0.428 - 0.835 - 4.18 - 9.20 mg/L.The concentrations of thetest itemBasic Red 76were analytically verified by HPLC-DAD at the start and the end of the exposure of all test concentrations and the control.The measured concentrations of the test item were between 32 and 94% of the nominal value at the start of the exposure (0 hours) and between 34 to 90% of the nominal value at the end of the exposure (72 hours). Therefore, all effect values given are based on the geometric mean test item concentrations of Basic Red 76. The EC50 values with 95% confidence intervals for inhibition of growth rate (ErC50) and yield (EyC50) after 72 hours were 0.802 (0.778-0.817)mg/L and 0.546 (0.502-0.625) mg/L respectively. The NOEC values for inhibition of growth rate and yield after 72 hours were 0.428 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.55 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
