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EC number: 613-782-9 | CAS number: 65355-32-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1996-08-05 to 1996-08-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Deviations:
- no
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: direct weighing in - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: laboratory plant treating predominantly municipal sewage (Südlicher Wupperverband)
- Pre-treatment: no
- pH of the culture solution before measuring the consumption : 6.5
- Initial biomass concentration: 800 mg/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.3 - 7.5
- Nominal and measured concentrations:
- Nominal conc.: 1000, 1800, 3200, 5600, 10000 mg/L;
no measured concentration. - Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST CONCENTRATIONS
1000, 1800, 3200, 5600, 10000 mg/L
Abiotic control: 10000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol, purity 99 %
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 8.8 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- After 30 min of exposure the EC50 was determined to be > 10000 mg/L.
- Executive summary:
The influence of the test item on the respiration rate of activated sludge was investigated after a contact time of 30 minutes in a study similar to OECD TG 209. Five concentrations were tested: 1000, 1800, 3200, 5600 and 10000 mg/L, while the abiotic control was run at the highest test substance concentration of 10000 mg/L. 3,5-dichlorophenol was used as the reference substance. After the 30-minutes exposure duration, for the reference substance 3,5-dichlorophenol an EC50 value of 8.8 mg/L was determined. The determination was carried out using a Probit analysis. Based on the measurement results, the test item showed a 31.4 % inhibition of the respiration of activated sludge at an application concentration of 10000 mg/L. Thus, the EC50 was determined to be > 10000 mg/L.
Reference
Results reference substance (3,5-dichlorophenol):
Concentration [mg/L] | Respiration rate [mg/L.h] | Inhibition [%] |
2.5 | 40.0 | 20.5 |
5 | 40.8 | 18.9 |
10 | 27.0 | 46.3 |
20 | 9.0 | 81.1 |
40 | 1.5 | 97.0 |
Results test substance
Concentration [mg/L] | Respiration rate [mg/L.h] | Inhibition [%] |
1000 | 34.8 | 30.8 |
1800 | 36.0 | 28.4 |
3200 | 31.2 | 38.0 |
5600 | 33.0 | 34.4 |
10000 | 34.5 | 31.4 |
Description of key information
A test on respiration inhibition using activated sludge of a predominantly domestic sewage treatment plant was conducted in a method similar to OECD 209. After 30 min of exposure the EC50 was determined to be > 10000 mg/L (reference 6.1.7-1).
Key value for chemical safety assessment
Additional information
The influence of the test item on the respiration rate of activated sludge was investigated after a contact time of 30 minutes in a study similar to OECD guideline 209. Five concentrations were tested: 1000, 1800, 3200, 5600 and 10000 mg/L, while the abiotic control was run at the highest test substance concentration of 10000 mg/L. 3,5-dichlorophenol was used as the reference substance. After the 30-minutes exposure duration, for the reference substance 3,5-dichlorophenol an EC50 value of 8.8 mg/L was determined. The determination was carried out using a probit analysis. Based on the measurement results, the test item shows a 31.4 % inhibition of the respiration of activated sludge at an application concentration of 10000 mg/L. Thus, the EC50 was determined to be > 10000 mg/L.
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